Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09430 and CA265_RS09435 are separated by 99 nucleotides CA265_RS09435 and CA265_RS09440 are separated by 26 nucleotides
CA265_RS09430: CA265_RS09430 - hypothetical protein, at 2,334,689 to 2,335,834
_RS09430
CA265_RS09435: CA265_RS09435 - hypothetical protein, at 2,335,934 to 2,337,022
_RS09435
CA265_RS09440: CA265_RS09440 - DNA gyrase subunit A, at 2,337,049 to 2,339,640
_RS09440
Position (kb)
2335
2336
2337
2338 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2334.988 kb on + strand, within CA265_RS09430 at 2335.012 kb on - strand, within CA265_RS09430 at 2335.012 kb on - strand, within CA265_RS09430 at 2335.027 kb on - strand, within CA265_RS09430 at 2335.117 kb on + strand, within CA265_RS09430 at 2335.120 kb on - strand, within CA265_RS09430 at 2335.141 kb on + strand, within CA265_RS09430 at 2335.209 kb on + strand, within CA265_RS09430 at 2335.275 kb on + strand, within CA265_RS09430 at 2335.317 kb on - strand, within CA265_RS09430 at 2335.319 kb on - strand, within CA265_RS09430 at 2335.325 kb on - strand, within CA265_RS09430 at 2335.325 kb on - strand, within CA265_RS09430 at 2335.406 kb on - strand, within CA265_RS09430 at 2335.633 kb on + strand, within CA265_RS09430 at 2335.677 kb on - strand, within CA265_RS09430 at 2335.681 kb on + strand, within CA265_RS09430 at 2335.732 kb on + strand at 2335.840 kb on - strand at 2336.192 kb on - strand, within CA265_RS09435 at 2336.415 kb on + strand, within CA265_RS09435 at 2336.415 kb on + strand, within CA265_RS09435 at 2336.415 kb on + strand, within CA265_RS09435 at 2336.417 kb on + strand, within CA265_RS09435 at 2336.417 kb on + strand, within CA265_RS09435 at 2336.517 kb on - strand, within CA265_RS09435 at 2336.546 kb on + strand, within CA265_RS09435 at 2336.629 kb on + strand, within CA265_RS09435 at 2336.681 kb on - strand, within CA265_RS09435 at 2336.750 kb on + strand, within CA265_RS09435 at 2337.047 kb on - strand at 2337.097 kb on - strand at 2337.118 kb on - strand at 2337.128 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 2,334,988 + CA265_RS09430 0.26 -0.1 2,335,012 - CA265_RS09430 0.28 +0.6 2,335,012 - CA265_RS09430 0.28 -0.7 2,335,027 - CA265_RS09430 0.29 -0.2 2,335,117 + CA265_RS09430 0.37 -0.1 2,335,120 - CA265_RS09430 0.38 -1.0 2,335,141 + CA265_RS09430 0.39 +0.3 2,335,209 + CA265_RS09430 0.45 -1.3 2,335,275 + CA265_RS09430 0.51 -1.2 2,335,317 - CA265_RS09430 0.55 -0.1 2,335,319 - CA265_RS09430 0.55 +0.4 2,335,325 - CA265_RS09430 0.55 -0.4 2,335,325 - CA265_RS09430 0.55 -0.3 2,335,406 - CA265_RS09430 0.63 -1.0 2,335,633 + CA265_RS09430 0.82 -1.8 2,335,677 - CA265_RS09430 0.86 -0.0 2,335,681 + CA265_RS09430 0.87 -0.9 2,335,732 + -0.6 2,335,840 - +0.2 2,336,192 - CA265_RS09435 0.24 -3.3 2,336,415 + CA265_RS09435 0.44 -1.8 2,336,415 + CA265_RS09435 0.44 -2.3 2,336,415 + CA265_RS09435 0.44 -0.2 2,336,417 + CA265_RS09435 0.44 +0.0 2,336,417 + CA265_RS09435 0.44 -0.9 2,336,517 - CA265_RS09435 0.54 -0.6 2,336,546 + CA265_RS09435 0.56 -2.6 2,336,629 + CA265_RS09435 0.64 +0.2 2,336,681 - CA265_RS09435 0.69 -2.6 2,336,750 + CA265_RS09435 0.75 -2.0 2,337,047 - +0.1 2,337,097 - +0.8 2,337,118 - -0.6 2,337,128 - +0.6
Or see this region's nucleotide sequence