Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09265 and CA265_RS09270 are separated by 779 nucleotides CA265_RS09270 and CA265_RS09275 are separated by 16 nucleotides CA265_RS09275 and CA265_RS09280 are separated by 131 nucleotides
CA265_RS09265: CA265_RS09265 - hypothetical protein, at 2,308,766 to 2,309,011
_RS09265
CA265_RS09270: CA265_RS09270 - hypothetical protein, at 2,309,791 to 2,310,042
_RS09270
CA265_RS09275: CA265_RS09275 - DUF3291 domain-containing protein, at 2,310,059 to 2,310,748
_RS09275
CA265_RS09280: CA265_RS09280 - diguanylate cyclase, at 2,310,880 to 2,311,359
_RS09280
Position (kb)
2309
2310
2311 Strain fitness (log2 ratio)
-2
-1
0
1 at 2308.805 kb on + strand, within CA265_RS09265 at 2308.806 kb on - strand, within CA265_RS09265 at 2308.830 kb on + strand, within CA265_RS09265 at 2308.922 kb on + strand, within CA265_RS09265 at 2308.944 kb on + strand, within CA265_RS09265 at 2308.996 kb on + strand at 2308.997 kb on - strand at 2309.050 kb on - strand at 2309.119 kb on + strand at 2309.120 kb on - strand at 2309.130 kb on + strand at 2309.136 kb on + strand at 2309.202 kb on + strand at 2309.203 kb on - strand at 2309.501 kb on - strand at 2309.503 kb on - strand at 2309.732 kb on + strand at 2309.796 kb on - strand at 2309.937 kb on - strand, within CA265_RS09270 at 2309.969 kb on - strand, within CA265_RS09270 at 2310.032 kb on - strand at 2310.074 kb on + strand at 2310.074 kb on + strand at 2310.097 kb on + strand at 2310.097 kb on + strand at 2310.133 kb on + strand, within CA265_RS09275 at 2310.133 kb on + strand, within CA265_RS09275 at 2310.133 kb on + strand, within CA265_RS09275 at 2310.160 kb on + strand, within CA265_RS09275 at 2310.160 kb on + strand, within CA265_RS09275 at 2310.190 kb on - strand, within CA265_RS09275 at 2310.196 kb on - strand, within CA265_RS09275 at 2310.281 kb on - strand, within CA265_RS09275 at 2310.296 kb on - strand, within CA265_RS09275 at 2310.398 kb on - strand, within CA265_RS09275 at 2310.398 kb on - strand, within CA265_RS09275 at 2310.464 kb on + strand, within CA265_RS09275 at 2310.465 kb on - strand, within CA265_RS09275 at 2310.499 kb on + strand, within CA265_RS09275 at 2310.562 kb on + strand, within CA265_RS09275 at 2310.643 kb on + strand, within CA265_RS09275 at 2310.705 kb on - strand at 2310.706 kb on + strand at 2310.710 kb on - strand at 2310.727 kb on + strand at 2310.795 kb on + strand at 2310.928 kb on + strand, within CA265_RS09280 at 2310.928 kb on + strand, within CA265_RS09280 at 2310.929 kb on - strand, within CA265_RS09280 at 2311.014 kb on + strand, within CA265_RS09280 at 2311.015 kb on - strand, within CA265_RS09280 at 2311.026 kb on + strand, within CA265_RS09280 at 2311.027 kb on - strand, within CA265_RS09280
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 2,308,805 + CA265_RS09265 0.16 -0.0 2,308,806 - CA265_RS09265 0.16 -0.3 2,308,830 + CA265_RS09265 0.26 -0.3 2,308,922 + CA265_RS09265 0.63 +1.0 2,308,944 + CA265_RS09265 0.72 +0.1 2,308,996 + -0.1 2,308,997 - -1.8 2,309,050 - +0.2 2,309,119 + -0.2 2,309,120 - -0.5 2,309,130 + +0.0 2,309,136 + +0.3 2,309,202 + -0.3 2,309,203 - -0.1 2,309,501 - -0.3 2,309,503 - -0.2 2,309,732 + +0.3 2,309,796 - +0.3 2,309,937 - CA265_RS09270 0.58 -0.7 2,309,969 - CA265_RS09270 0.71 -0.9 2,310,032 - -0.1 2,310,074 + -0.6 2,310,074 + -0.2 2,310,097 + +0.0 2,310,097 + +0.2 2,310,133 + CA265_RS09275 0.11 +0.4 2,310,133 + CA265_RS09275 0.11 -0.1 2,310,133 + CA265_RS09275 0.11 -0.6 2,310,160 + CA265_RS09275 0.15 +0.2 2,310,160 + CA265_RS09275 0.15 -0.5 2,310,190 - CA265_RS09275 0.19 -1.1 2,310,196 - CA265_RS09275 0.20 +0.1 2,310,281 - CA265_RS09275 0.32 +0.5 2,310,296 - CA265_RS09275 0.34 +0.0 2,310,398 - CA265_RS09275 0.49 +0.5 2,310,398 - CA265_RS09275 0.49 +0.2 2,310,464 + CA265_RS09275 0.59 -0.2 2,310,465 - CA265_RS09275 0.59 -0.6 2,310,499 + CA265_RS09275 0.64 -0.1 2,310,562 + CA265_RS09275 0.73 -0.6 2,310,643 + CA265_RS09275 0.85 +0.0 2,310,705 - -0.0 2,310,706 + +0.3 2,310,710 - +0.6 2,310,727 + -0.2 2,310,795 + -0.0 2,310,928 + CA265_RS09280 0.10 +0.1 2,310,928 + CA265_RS09280 0.10 -1.2 2,310,929 - CA265_RS09280 0.10 -0.2 2,311,014 + CA265_RS09280 0.28 -0.1 2,311,015 - CA265_RS09280 0.28 -0.1 2,311,026 + CA265_RS09280 0.30 +0.2 2,311,027 - CA265_RS09280 0.31 -0.4
Or see this region's nucleotide sequence