Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09005 and CA265_RS09010 are separated by 79 nucleotides CA265_RS09010 and CA265_RS09015 are separated by 233 nucleotides CA265_RS09015 and CA265_RS09020 are separated by 168 nucleotides
CA265_RS09005: CA265_RS09005 - phosphoserine transaminase, at 2,246,240 to 2,247,313
_RS09005
CA265_RS09010: CA265_RS09010 - 3-phosphoglycerate dehydrogenase, at 2,247,393 to 2,248,340
_RS09010
CA265_RS09015: CA265_RS09015 - hypothetical protein, at 2,248,574 to 2,249,098
_RS09015
CA265_RS09020: CA265_RS09020 - hypothetical protein, at 2,249,267 to 2,250,046
_RS09020
Position (kb)
2247
2248
2249 Strain fitness (log2 ratio)
-2
-1
0
1 at 2247.347 kb on - strand at 2247.386 kb on + strand at 2247.587 kb on - strand, within CA265_RS09010 at 2247.617 kb on + strand, within CA265_RS09010 at 2247.630 kb on + strand, within CA265_RS09010 at 2247.713 kb on - strand, within CA265_RS09010 at 2247.799 kb on - strand, within CA265_RS09010 at 2247.836 kb on + strand, within CA265_RS09010 at 2247.867 kb on - strand, within CA265_RS09010 at 2247.916 kb on + strand, within CA265_RS09010 at 2247.916 kb on + strand, within CA265_RS09010 at 2247.916 kb on + strand, within CA265_RS09010 at 2247.917 kb on - strand, within CA265_RS09010 at 2248.008 kb on - strand, within CA265_RS09010 at 2248.008 kb on - strand, within CA265_RS09010 at 2248.030 kb on + strand, within CA265_RS09010 at 2248.100 kb on + strand, within CA265_RS09010 at 2248.101 kb on - strand, within CA265_RS09010 at 2248.156 kb on + strand, within CA265_RS09010 at 2248.259 kb on + strand at 2248.260 kb on - strand at 2248.260 kb on - strand at 2248.341 kb on + strand at 2248.360 kb on + strand at 2248.528 kb on + strand at 2248.582 kb on + strand at 2248.583 kb on - strand at 2248.583 kb on - strand at 2248.607 kb on + strand at 2248.666 kb on + strand, within CA265_RS09015 at 2248.669 kb on - strand, within CA265_RS09015 at 2248.669 kb on - strand, within CA265_RS09015 at 2248.669 kb on - strand, within CA265_RS09015 at 2248.754 kb on - strand, within CA265_RS09015 at 2248.755 kb on + strand, within CA265_RS09015 at 2248.756 kb on - strand, within CA265_RS09015 at 2248.780 kb on + strand, within CA265_RS09015 at 2248.819 kb on + strand, within CA265_RS09015 at 2248.822 kb on - strand, within CA265_RS09015 at 2248.866 kb on - strand, within CA265_RS09015 at 2248.959 kb on + strand, within CA265_RS09015 at 2249.005 kb on + strand, within CA265_RS09015 at 2249.021 kb on - strand, within CA265_RS09015 at 2249.022 kb on + strand, within CA265_RS09015 at 2249.033 kb on - strand, within CA265_RS09015 at 2249.056 kb on + strand at 2249.056 kb on + strand at 2249.066 kb on + strand at 2249.071 kb on + strand at 2249.082 kb on + strand at 2249.143 kb on + strand at 2249.166 kb on - strand at 2249.213 kb on - strand at 2249.222 kb on + strand at 2249.235 kb on + strand at 2249.268 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 2,247,347 - -0.4 2,247,386 + -1.5 2,247,587 - CA265_RS09010 0.20 -2.3 2,247,617 + CA265_RS09010 0.24 -0.3 2,247,630 + CA265_RS09010 0.25 +1.4 2,247,713 - CA265_RS09010 0.34 -1.3 2,247,799 - CA265_RS09010 0.43 -0.8 2,247,836 + CA265_RS09010 0.47 +0.3 2,247,867 - CA265_RS09010 0.50 +0.0 2,247,916 + CA265_RS09010 0.55 +1.0 2,247,916 + CA265_RS09010 0.55 -1.7 2,247,916 + CA265_RS09010 0.55 -2.2 2,247,917 - CA265_RS09010 0.55 +0.3 2,248,008 - CA265_RS09010 0.65 -1.0 2,248,008 - CA265_RS09010 0.65 -1.5 2,248,030 + CA265_RS09010 0.67 -1.1 2,248,100 + CA265_RS09010 0.75 -1.2 2,248,101 - CA265_RS09010 0.75 +0.5 2,248,156 + CA265_RS09010 0.80 +1.0 2,248,259 + -0.6 2,248,260 - -0.3 2,248,260 - +0.6 2,248,341 + -0.8 2,248,360 + +1.0 2,248,528 + +0.6 2,248,582 + +0.4 2,248,583 - -1.2 2,248,583 - -1.0 2,248,607 + +0.3 2,248,666 + CA265_RS09015 0.18 +0.6 2,248,669 - CA265_RS09015 0.18 +0.2 2,248,669 - CA265_RS09015 0.18 -0.9 2,248,669 - CA265_RS09015 0.18 -0.4 2,248,754 - CA265_RS09015 0.34 -0.6 2,248,755 + CA265_RS09015 0.34 -0.3 2,248,756 - CA265_RS09015 0.35 -0.1 2,248,780 + CA265_RS09015 0.39 -0.3 2,248,819 + CA265_RS09015 0.47 -0.5 2,248,822 - CA265_RS09015 0.47 -0.4 2,248,866 - CA265_RS09015 0.56 -0.9 2,248,959 + CA265_RS09015 0.73 -0.3 2,249,005 + CA265_RS09015 0.82 +0.5 2,249,021 - CA265_RS09015 0.85 -0.1 2,249,022 + CA265_RS09015 0.85 +0.2 2,249,033 - CA265_RS09015 0.87 +0.1 2,249,056 + +0.7 2,249,056 + -0.1 2,249,066 + +0.9 2,249,071 + -0.3 2,249,082 + -0.5 2,249,143 + -0.7 2,249,166 - -0.2 2,249,213 - +0.0 2,249,222 + +0.4 2,249,235 + -0.6 2,249,268 + +0.2
Or see this region's nucleotide sequence