Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08950 and CA265_RS08955 are separated by 84 nucleotides CA265_RS08955 and CA265_RS08960 are separated by 331 nucleotides
CA265_RS08950: CA265_RS08950 - bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/5,10-methylene-tetrahydrofolate cyclohydrolase, at 2,232,938 to 2,233,858
_RS08950
CA265_RS08955: CA265_RS08955 - elongation factor 4, at 2,233,943 to 2,235,730
_RS08955
CA265_RS08960: CA265_RS08960 - MATE family efflux transporter, at 2,236,062 to 2,237,486
_RS08960
Position (kb)
2233
2234
2235
2236 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2232.945 kb on + strand at 2232.945 kb on + strand at 2234.060 kb on - strand at 2234.279 kb on - strand, within CA265_RS08955 at 2234.611 kb on - strand, within CA265_RS08955 at 2234.948 kb on - strand, within CA265_RS08955 at 2234.960 kb on - strand, within CA265_RS08955 at 2235.005 kb on - strand, within CA265_RS08955 at 2235.040 kb on - strand, within CA265_RS08955 at 2235.106 kb on - strand, within CA265_RS08955 at 2235.387 kb on - strand, within CA265_RS08955 at 2235.387 kb on - strand, within CA265_RS08955 at 2235.650 kb on - strand at 2235.695 kb on - strand at 2235.716 kb on - strand at 2235.854 kb on - strand at 2235.860 kb on + strand at 2235.882 kb on + strand at 2235.883 kb on - strand at 2235.931 kb on - strand at 2235.944 kb on - strand at 2235.982 kb on - strand at 2236.035 kb on + strand at 2236.094 kb on + strand at 2236.192 kb on + strand at 2236.280 kb on + strand, within CA265_RS08960 at 2236.442 kb on - strand, within CA265_RS08960 at 2236.479 kb on + strand, within CA265_RS08960 at 2236.480 kb on - strand, within CA265_RS08960 at 2236.546 kb on - strand, within CA265_RS08960 at 2236.551 kb on - strand, within CA265_RS08960 at 2236.643 kb on + strand, within CA265_RS08960 at 2236.644 kb on - strand, within CA265_RS08960 at 2236.656 kb on + strand, within CA265_RS08960 at 2236.656 kb on + strand, within CA265_RS08960 at 2236.688 kb on + strand, within CA265_RS08960 at 2236.689 kb on - strand, within CA265_RS08960
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 2,232,945 + +0.5 2,232,945 + +0.6 2,234,060 - +2.3 2,234,279 - CA265_RS08955 0.19 -0.8 2,234,611 - CA265_RS08955 0.37 -2.0 2,234,948 - CA265_RS08955 0.56 -1.0 2,234,960 - CA265_RS08955 0.57 -1.5 2,235,005 - CA265_RS08955 0.59 -2.3 2,235,040 - CA265_RS08955 0.61 -0.8 2,235,106 - CA265_RS08955 0.65 -1.6 2,235,387 - CA265_RS08955 0.81 +0.4 2,235,387 - CA265_RS08955 0.81 -1.0 2,235,650 - -0.6 2,235,695 - -0.7 2,235,716 - -0.6 2,235,854 - -0.1 2,235,860 + +0.1 2,235,882 + +1.0 2,235,883 - -1.1 2,235,931 - +0.2 2,235,944 - +1.0 2,235,982 - +0.3 2,236,035 + +0.1 2,236,094 + +0.5 2,236,192 + -0.0 2,236,280 + CA265_RS08960 0.15 -0.1 2,236,442 - CA265_RS08960 0.27 -0.8 2,236,479 + CA265_RS08960 0.29 +0.1 2,236,480 - CA265_RS08960 0.29 +0.7 2,236,546 - CA265_RS08960 0.34 +0.1 2,236,551 - CA265_RS08960 0.34 +0.1 2,236,643 + CA265_RS08960 0.41 +0.1 2,236,644 - CA265_RS08960 0.41 +0.3 2,236,656 + CA265_RS08960 0.42 +0.1 2,236,656 + CA265_RS08960 0.42 +0.1 2,236,688 + CA265_RS08960 0.44 +0.2 2,236,689 - CA265_RS08960 0.44 +1.0
Or see this region's nucleotide sequence