Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08900 and CA265_RS08905 are separated by 91 nucleotides CA265_RS08905 and CA265_RS08910 are separated by 85 nucleotides CA265_RS08910 and CA265_RS08915 are separated by 2 nucleotides
CA265_RS08900: CA265_RS08900 - D-arabinose 5-phosphate isomerase, at 2,223,664 to 2,224,632
_RS08900
CA265_RS08905: CA265_RS08905 - DNA helicase RecQ, at 2,224,724 to 2,226,913
_RS08905
CA265_RS08910: CA265_RS08910 - 3-deoxy-8-phosphooctulonate synthase, at 2,226,999 to 2,227,811
_RS08910
CA265_RS08915: CA265_RS08915 - hypothetical protein, at 2,227,814 to 2,228,528
_RS08915
Position (kb)
2224
2225
2226
2227 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2223.734 kb on - strand at 2223.800 kb on - strand, within CA265_RS08900 at 2223.838 kb on + strand, within CA265_RS08900 at 2223.858 kb on + strand, within CA265_RS08900 at 2223.858 kb on + strand, within CA265_RS08900 at 2224.096 kb on - strand, within CA265_RS08900 at 2224.158 kb on + strand, within CA265_RS08900 at 2224.307 kb on - strand, within CA265_RS08900 at 2224.353 kb on + strand, within CA265_RS08900 at 2224.353 kb on + strand, within CA265_RS08900 at 2224.354 kb on - strand, within CA265_RS08900 at 2224.422 kb on + strand, within CA265_RS08900 at 2224.492 kb on + strand, within CA265_RS08900 at 2224.493 kb on - strand, within CA265_RS08900 at 2224.546 kb on - strand at 2224.564 kb on + strand at 2224.598 kb on - strand at 2224.652 kb on + strand at 2224.682 kb on - strand at 2224.756 kb on - strand at 2224.950 kb on + strand, within CA265_RS08905 at 2224.984 kb on + strand, within CA265_RS08905 at 2225.062 kb on + strand, within CA265_RS08905 at 2225.249 kb on - strand, within CA265_RS08905 at 2225.270 kb on - strand, within CA265_RS08905 at 2225.294 kb on - strand at 2225.329 kb on + strand, within CA265_RS08905 at 2225.402 kb on - strand, within CA265_RS08905 at 2225.437 kb on + strand, within CA265_RS08905 at 2225.491 kb on - strand, within CA265_RS08905 at 2225.514 kb on - strand, within CA265_RS08905 at 2225.608 kb on + strand, within CA265_RS08905 at 2225.675 kb on + strand at 2225.831 kb on + strand, within CA265_RS08905 at 2226.162 kb on - strand, within CA265_RS08905 at 2226.223 kb on + strand, within CA265_RS08905 at 2226.237 kb on - strand, within CA265_RS08905 at 2226.325 kb on + strand, within CA265_RS08905 at 2226.325 kb on + strand, within CA265_RS08905 at 2226.477 kb on + strand, within CA265_RS08905 at 2226.577 kb on + strand, within CA265_RS08905 at 2226.903 kb on + strand at 2226.994 kb on + strand at 2226.995 kb on - strand at 2226.999 kb on - strand at 2227.012 kb on + strand at 2227.085 kb on + strand, within CA265_RS08910 at 2227.182 kb on - strand, within CA265_RS08910 at 2227.268 kb on + strand, within CA265_RS08910 at 2227.269 kb on - strand, within CA265_RS08910 at 2227.321 kb on + strand, within CA265_RS08910 at 2227.322 kb on - strand, within CA265_RS08910 at 2227.496 kb on + strand, within CA265_RS08910 at 2227.583 kb on + strand, within CA265_RS08910 at 2227.621 kb on + strand, within CA265_RS08910 at 2227.690 kb on + strand, within CA265_RS08910 at 2227.742 kb on + strand at 2227.743 kb on - strand at 2227.747 kb on + strand at 2227.775 kb on - strand at 2227.802 kb on + strand at 2227.802 kb on + strand at 2227.803 kb on - strand at 2227.872 kb on - strand at 2227.890 kb on + strand, within CA265_RS08915
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 2,223,734 - -1.4 2,223,800 - CA265_RS08900 0.14 -0.8 2,223,838 + CA265_RS08900 0.18 -1.8 2,223,858 + CA265_RS08900 0.20 +0.3 2,223,858 + CA265_RS08900 0.20 -1.1 2,224,096 - CA265_RS08900 0.45 -1.4 2,224,158 + CA265_RS08900 0.51 -1.2 2,224,307 - CA265_RS08900 0.66 -1.2 2,224,353 + CA265_RS08900 0.71 -1.0 2,224,353 + CA265_RS08900 0.71 -1.7 2,224,354 - CA265_RS08900 0.71 +0.2 2,224,422 + CA265_RS08900 0.78 -1.1 2,224,492 + CA265_RS08900 0.85 -0.8 2,224,493 - CA265_RS08900 0.86 -0.1 2,224,546 - +0.1 2,224,564 + -0.8 2,224,598 - -1.5 2,224,652 + +0.2 2,224,682 - -0.7 2,224,756 - -0.6 2,224,950 + CA265_RS08905 0.10 -0.5 2,224,984 + CA265_RS08905 0.12 -2.0 2,225,062 + CA265_RS08905 0.15 -0.4 2,225,249 - CA265_RS08905 0.24 -1.8 2,225,270 - CA265_RS08905 0.25 -2.5 2,225,294 - -1.3 2,225,329 + CA265_RS08905 0.28 -1.0 2,225,402 - CA265_RS08905 0.31 -1.8 2,225,437 + CA265_RS08905 0.33 -0.7 2,225,491 - CA265_RS08905 0.35 -0.1 2,225,514 - CA265_RS08905 0.36 -0.8 2,225,608 + CA265_RS08905 0.40 -0.8 2,225,675 + +1.0 2,225,831 + CA265_RS08905 0.51 -1.1 2,226,162 - CA265_RS08905 0.66 -2.0 2,226,223 + CA265_RS08905 0.68 +1.6 2,226,237 - CA265_RS08905 0.69 +1.7 2,226,325 + CA265_RS08905 0.73 -0.6 2,226,325 + CA265_RS08905 0.73 +0.3 2,226,477 + CA265_RS08905 0.80 -0.6 2,226,577 + CA265_RS08905 0.85 +0.4 2,226,903 + -0.0 2,226,994 + +0.7 2,226,995 - +0.6 2,226,999 - +0.3 2,227,012 + -1.6 2,227,085 + CA265_RS08910 0.11 -3.0 2,227,182 - CA265_RS08910 0.23 -0.7 2,227,268 + CA265_RS08910 0.33 +0.2 2,227,269 - CA265_RS08910 0.33 -1.6 2,227,321 + CA265_RS08910 0.40 -0.5 2,227,322 - CA265_RS08910 0.40 -1.5 2,227,496 + CA265_RS08910 0.61 -1.0 2,227,583 + CA265_RS08910 0.72 -2.0 2,227,621 + CA265_RS08910 0.77 -1.7 2,227,690 + CA265_RS08910 0.85 -0.8 2,227,742 + -1.4 2,227,743 - -0.4 2,227,747 + -2.0 2,227,775 - -1.3 2,227,802 + +0.1 2,227,802 + +0.2 2,227,803 - -1.7 2,227,872 - -0.8 2,227,890 + CA265_RS08915 0.11 +0.1
Or see this region's nucleotide sequence