Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08895 and CA265_RS08900 are separated by 14 nucleotides CA265_RS08900 and CA265_RS08905 are separated by 91 nucleotides
CA265_RS08895: CA265_RS08895 - mannose-1-phosphate guanylyltransferase, at 2,222,558 to 2,223,649
_RS08895
CA265_RS08900: CA265_RS08900 - D-arabinose 5-phosphate isomerase, at 2,223,664 to 2,224,632
_RS08900
CA265_RS08905: CA265_RS08905 - DNA helicase RecQ, at 2,224,724 to 2,226,913
_RS08905
Position (kb)
2223
2224
2225 Strain fitness (log2 ratio)
-2
-1
0
1 at 2222.748 kb on - strand, within CA265_RS08895 at 2222.807 kb on - strand, within CA265_RS08895 at 2222.856 kb on + strand, within CA265_RS08895 at 2222.916 kb on + strand, within CA265_RS08895 at 2222.916 kb on + strand, within CA265_RS08895 at 2222.924 kb on - strand, within CA265_RS08895 at 2222.979 kb on - strand, within CA265_RS08895 at 2222.986 kb on - strand, within CA265_RS08895 at 2222.986 kb on - strand, within CA265_RS08895 at 2223.017 kb on + strand, within CA265_RS08895 at 2223.130 kb on + strand, within CA265_RS08895 at 2223.149 kb on - strand, within CA265_RS08895 at 2223.159 kb on + strand, within CA265_RS08895 at 2223.176 kb on - strand, within CA265_RS08895 at 2223.215 kb on + strand, within CA265_RS08895 at 2223.251 kb on + strand, within CA265_RS08895 at 2223.265 kb on + strand, within CA265_RS08895 at 2223.272 kb on + strand, within CA265_RS08895 at 2223.273 kb on - strand, within CA265_RS08895 at 2223.274 kb on + strand, within CA265_RS08895 at 2223.299 kb on - strand, within CA265_RS08895 at 2223.339 kb on - strand, within CA265_RS08895 at 2223.371 kb on - strand, within CA265_RS08895 at 2223.393 kb on - strand, within CA265_RS08895 at 2223.469 kb on + strand, within CA265_RS08895 at 2223.527 kb on + strand, within CA265_RS08895 at 2223.528 kb on - strand, within CA265_RS08895 at 2223.542 kb on + strand at 2223.559 kb on + strand at 2223.587 kb on + strand at 2223.627 kb on - strand at 2223.665 kb on + strand at 2223.672 kb on + strand at 2223.714 kb on - strand at 2223.734 kb on - strand at 2223.800 kb on - strand, within CA265_RS08900 at 2223.838 kb on + strand, within CA265_RS08900 at 2223.858 kb on + strand, within CA265_RS08900 at 2223.858 kb on + strand, within CA265_RS08900 at 2224.096 kb on - strand, within CA265_RS08900 at 2224.158 kb on + strand, within CA265_RS08900 at 2224.307 kb on - strand, within CA265_RS08900 at 2224.353 kb on + strand, within CA265_RS08900 at 2224.353 kb on + strand, within CA265_RS08900 at 2224.354 kb on - strand, within CA265_RS08900 at 2224.422 kb on + strand, within CA265_RS08900 at 2224.492 kb on + strand, within CA265_RS08900 at 2224.493 kb on - strand, within CA265_RS08900 at 2224.546 kb on - strand at 2224.564 kb on + strand at 2224.598 kb on - strand at 2224.652 kb on + strand at 2224.682 kb on - strand at 2224.756 kb on - strand at 2224.950 kb on + strand, within CA265_RS08905 at 2224.984 kb on + strand, within CA265_RS08905 at 2225.062 kb on + strand, within CA265_RS08905 at 2225.249 kb on - strand, within CA265_RS08905 at 2225.270 kb on - strand, within CA265_RS08905 at 2225.294 kb on - strand at 2225.329 kb on + strand, within CA265_RS08905 at 2225.402 kb on - strand, within CA265_RS08905 at 2225.437 kb on + strand, within CA265_RS08905 at 2225.491 kb on - strand, within CA265_RS08905 at 2225.514 kb on - strand, within CA265_RS08905 at 2225.608 kb on + strand, within CA265_RS08905
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 2,222,748 - CA265_RS08895 0.17 +0.1 2,222,807 - CA265_RS08895 0.23 +0.5 2,222,856 + CA265_RS08895 0.27 +0.4 2,222,916 + CA265_RS08895 0.33 -1.2 2,222,916 + CA265_RS08895 0.33 +0.5 2,222,924 - CA265_RS08895 0.34 +1.2 2,222,979 - CA265_RS08895 0.39 -0.0 2,222,986 - CA265_RS08895 0.39 -1.6 2,222,986 - CA265_RS08895 0.39 -0.0 2,223,017 + CA265_RS08895 0.42 +0.4 2,223,130 + CA265_RS08895 0.52 +0.4 2,223,149 - CA265_RS08895 0.54 -1.0 2,223,159 + CA265_RS08895 0.55 +0.3 2,223,176 - CA265_RS08895 0.57 +0.1 2,223,215 + CA265_RS08895 0.60 +0.8 2,223,251 + CA265_RS08895 0.63 -0.1 2,223,265 + CA265_RS08895 0.65 -0.7 2,223,272 + CA265_RS08895 0.65 +0.3 2,223,273 - CA265_RS08895 0.65 +0.1 2,223,274 + CA265_RS08895 0.66 -0.5 2,223,299 - CA265_RS08895 0.68 +0.0 2,223,339 - CA265_RS08895 0.72 -0.4 2,223,371 - CA265_RS08895 0.74 +0.6 2,223,393 - CA265_RS08895 0.76 -0.1 2,223,469 + CA265_RS08895 0.83 -0.5 2,223,527 + CA265_RS08895 0.89 +0.5 2,223,528 - CA265_RS08895 0.89 +0.1 2,223,542 + -1.6 2,223,559 + -0.1 2,223,587 + +0.0 2,223,627 - +0.0 2,223,665 + -0.2 2,223,672 + -1.3 2,223,714 - -2.3 2,223,734 - -1.4 2,223,800 - CA265_RS08900 0.14 -0.8 2,223,838 + CA265_RS08900 0.18 -1.8 2,223,858 + CA265_RS08900 0.20 +0.3 2,223,858 + CA265_RS08900 0.20 -1.1 2,224,096 - CA265_RS08900 0.45 -1.4 2,224,158 + CA265_RS08900 0.51 -1.2 2,224,307 - CA265_RS08900 0.66 -1.2 2,224,353 + CA265_RS08900 0.71 -1.0 2,224,353 + CA265_RS08900 0.71 -1.7 2,224,354 - CA265_RS08900 0.71 +0.2 2,224,422 + CA265_RS08900 0.78 -1.1 2,224,492 + CA265_RS08900 0.85 -0.8 2,224,493 - CA265_RS08900 0.86 -0.1 2,224,546 - +0.1 2,224,564 + -0.8 2,224,598 - -1.5 2,224,652 + +0.2 2,224,682 - -0.7 2,224,756 - -0.6 2,224,950 + CA265_RS08905 0.10 -0.5 2,224,984 + CA265_RS08905 0.12 -2.0 2,225,062 + CA265_RS08905 0.15 -0.4 2,225,249 - CA265_RS08905 0.24 -1.8 2,225,270 - CA265_RS08905 0.25 -2.5 2,225,294 - -1.3 2,225,329 + CA265_RS08905 0.28 -1.0 2,225,402 - CA265_RS08905 0.31 -1.8 2,225,437 + CA265_RS08905 0.33 -0.7 2,225,491 - CA265_RS08905 0.35 -0.1 2,225,514 - CA265_RS08905 0.36 -0.8 2,225,608 + CA265_RS08905 0.40 -0.8
Or see this region's nucleotide sequence