Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08085 and CA265_RS08090 are separated by 18 nucleotides CA265_RS08090 and CA265_RS08095 are separated by 213 nucleotides
CA265_RS08085: CA265_RS08085 - aminodeoxychorismate lyase, at 2,009,427 to 2,010,482
_RS08085
CA265_RS08090: CA265_RS08090 - serine/threonine protein kinase, at 2,010,501 to 2,011,271
_RS08090
CA265_RS08095: CA265_RS08095 - D-alanine--D-alanine ligase A, at 2,011,485 to 2,012,471
_RS08095
Position (kb)
2010
2011
2012 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2009.545 kb on + strand, within CA265_RS08085 at 2009.549 kb on + strand, within CA265_RS08085 at 2009.550 kb on - strand, within CA265_RS08085 at 2009.552 kb on - strand, within CA265_RS08085 at 2009.595 kb on - strand, within CA265_RS08085 at 2009.636 kb on + strand, within CA265_RS08085 at 2009.636 kb on + strand, within CA265_RS08085 at 2009.636 kb on + strand, within CA265_RS08085 at 2009.637 kb on - strand, within CA265_RS08085 at 2009.637 kb on - strand, within CA265_RS08085 at 2009.638 kb on + strand, within CA265_RS08085 at 2009.648 kb on - strand, within CA265_RS08085 at 2009.648 kb on - strand, within CA265_RS08085 at 2009.659 kb on - strand, within CA265_RS08085 at 2009.733 kb on + strand, within CA265_RS08085 at 2009.775 kb on + strand, within CA265_RS08085 at 2009.836 kb on - strand, within CA265_RS08085 at 2009.836 kb on - strand, within CA265_RS08085 at 2009.836 kb on - strand, within CA265_RS08085 at 2009.841 kb on + strand, within CA265_RS08085 at 2009.857 kb on - strand, within CA265_RS08085 at 2009.898 kb on - strand, within CA265_RS08085 at 2009.903 kb on - strand, within CA265_RS08085 at 2009.903 kb on - strand, within CA265_RS08085 at 2009.999 kb on + strand, within CA265_RS08085 at 2010.156 kb on + strand, within CA265_RS08085 at 2010.161 kb on + strand, within CA265_RS08085 at 2010.166 kb on - strand, within CA265_RS08085 at 2010.334 kb on - strand, within CA265_RS08085 at 2010.430 kb on - strand at 2010.472 kb on - strand at 2010.486 kb on + strand at 2010.492 kb on - strand at 2010.494 kb on + strand at 2010.520 kb on - strand at 2010.525 kb on - strand at 2010.543 kb on + strand at 2010.575 kb on + strand at 2010.575 kb on + strand at 2010.576 kb on - strand at 2010.591 kb on - strand, within CA265_RS08090 at 2010.591 kb on - strand, within CA265_RS08090 at 2010.691 kb on - strand, within CA265_RS08090 at 2010.711 kb on - strand, within CA265_RS08090 at 2011.295 kb on + strand at 2011.295 kb on + strand at 2011.296 kb on - strand at 2011.296 kb on - strand at 2011.335 kb on - strand at 2011.450 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 2,009,545 + CA265_RS08085 0.11 -4.8 2,009,549 + CA265_RS08085 0.12 -2.9 2,009,550 - CA265_RS08085 0.12 -2.7 2,009,552 - CA265_RS08085 0.12 -1.0 2,009,595 - CA265_RS08085 0.16 -2.6 2,009,636 + CA265_RS08085 0.20 -1.8 2,009,636 + CA265_RS08085 0.20 -3.9 2,009,636 + CA265_RS08085 0.20 -3.6 2,009,637 - CA265_RS08085 0.20 -0.6 2,009,637 - CA265_RS08085 0.20 -1.5 2,009,638 + CA265_RS08085 0.20 -2.7 2,009,648 - CA265_RS08085 0.21 -2.7 2,009,648 - CA265_RS08085 0.21 -1.4 2,009,659 - CA265_RS08085 0.22 -0.6 2,009,733 + CA265_RS08085 0.29 -1.7 2,009,775 + CA265_RS08085 0.33 -3.3 2,009,836 - CA265_RS08085 0.39 -0.9 2,009,836 - CA265_RS08085 0.39 -1.4 2,009,836 - CA265_RS08085 0.39 -1.0 2,009,841 + CA265_RS08085 0.39 -2.6 2,009,857 - CA265_RS08085 0.41 -1.6 2,009,898 - CA265_RS08085 0.45 -0.6 2,009,903 - CA265_RS08085 0.45 -0.8 2,009,903 - CA265_RS08085 0.45 -1.9 2,009,999 + CA265_RS08085 0.54 -1.8 2,010,156 + CA265_RS08085 0.69 -2.9 2,010,161 + CA265_RS08085 0.70 -5.4 2,010,166 - CA265_RS08085 0.70 -1.6 2,010,334 - CA265_RS08085 0.86 -1.0 2,010,430 - -1.7 2,010,472 - +0.5 2,010,486 + -2.9 2,010,492 - +1.2 2,010,494 + -1.3 2,010,520 - -2.4 2,010,525 - -4.1 2,010,543 + -3.3 2,010,575 + -3.0 2,010,575 + -2.8 2,010,576 - -3.0 2,010,591 - CA265_RS08090 0.12 -4.8 2,010,591 - CA265_RS08090 0.12 -4.4 2,010,691 - CA265_RS08090 0.25 -4.6 2,010,711 - CA265_RS08090 0.27 -2.5 2,011,295 + -3.8 2,011,295 + -3.6 2,011,296 - +0.4 2,011,296 - -0.3 2,011,335 - +0.1 2,011,450 + -0.2
Or see this region's nucleotide sequence