Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08075 and CA265_RS08080 are separated by 8 nucleotides CA265_RS08080 and CA265_RS08085 overlap by 1 nucleotides CA265_RS08085 and CA265_RS08090 are separated by 18 nucleotides
CA265_RS08075: CA265_RS08075 - ribonuclease BN, at 2,008,022 to 2,009,011
_RS08075
CA265_RS08080: CA265_RS08080 - thioesterase, at 2,009,020 to 2,009,427
_RS08080
CA265_RS08085: CA265_RS08085 - aminodeoxychorismate lyase, at 2,009,427 to 2,010,482
_RS08085
CA265_RS08090: CA265_RS08090 - serine/threonine protein kinase, at 2,010,501 to 2,011,271
_RS08090
Position (kb)
2009
2010
2011 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 2008.521 kb on - strand, within CA265_RS08075 at 2008.535 kb on + strand, within CA265_RS08075 at 2008.787 kb on + strand, within CA265_RS08075 at 2008.901 kb on + strand, within CA265_RS08075 at 2008.902 kb on - strand, within CA265_RS08075 at 2008.902 kb on - strand, within CA265_RS08075 at 2008.908 kb on + strand, within CA265_RS08075 at 2008.967 kb on - strand at 2008.971 kb on + strand at 2008.978 kb on + strand at 2008.979 kb on - strand at 2008.989 kb on - strand at 2009.001 kb on - strand at 2009.118 kb on + strand, within CA265_RS08080 at 2009.145 kb on - strand, within CA265_RS08080 at 2009.154 kb on - strand, within CA265_RS08080 at 2009.235 kb on + strand, within CA265_RS08080 at 2009.241 kb on + strand, within CA265_RS08080 at 2009.294 kb on + strand, within CA265_RS08080 at 2009.294 kb on + strand, within CA265_RS08080 at 2009.343 kb on - strand, within CA265_RS08080 at 2009.364 kb on - strand, within CA265_RS08080 at 2009.364 kb on - strand, within CA265_RS08080 at 2009.364 kb on - strand, within CA265_RS08080 at 2009.368 kb on - strand, within CA265_RS08080 at 2009.368 kb on - strand, within CA265_RS08080 at 2009.368 kb on - strand, within CA265_RS08080 at 2009.368 kb on - strand, within CA265_RS08080 at 2009.413 kb on - strand at 2009.422 kb on - strand at 2009.429 kb on - strand at 2009.433 kb on - strand at 2009.436 kb on + strand at 2009.545 kb on + strand, within CA265_RS08085 at 2009.549 kb on + strand, within CA265_RS08085 at 2009.550 kb on - strand, within CA265_RS08085 at 2009.552 kb on - strand, within CA265_RS08085 at 2009.595 kb on - strand, within CA265_RS08085 at 2009.636 kb on + strand, within CA265_RS08085 at 2009.636 kb on + strand, within CA265_RS08085 at 2009.636 kb on + strand, within CA265_RS08085 at 2009.637 kb on - strand, within CA265_RS08085 at 2009.637 kb on - strand, within CA265_RS08085 at 2009.638 kb on + strand, within CA265_RS08085 at 2009.648 kb on - strand, within CA265_RS08085 at 2009.648 kb on - strand, within CA265_RS08085 at 2009.659 kb on - strand, within CA265_RS08085 at 2009.733 kb on + strand, within CA265_RS08085 at 2009.775 kb on + strand, within CA265_RS08085 at 2009.836 kb on - strand, within CA265_RS08085 at 2009.836 kb on - strand, within CA265_RS08085 at 2009.836 kb on - strand, within CA265_RS08085 at 2009.841 kb on + strand, within CA265_RS08085 at 2009.857 kb on - strand, within CA265_RS08085 at 2009.898 kb on - strand, within CA265_RS08085 at 2009.903 kb on - strand, within CA265_RS08085 at 2009.903 kb on - strand, within CA265_RS08085 at 2009.999 kb on + strand, within CA265_RS08085 at 2010.156 kb on + strand, within CA265_RS08085 at 2010.161 kb on + strand, within CA265_RS08085 at 2010.166 kb on - strand, within CA265_RS08085 at 2010.334 kb on - strand, within CA265_RS08085 at 2010.430 kb on - strand at 2010.472 kb on - strand at 2010.486 kb on + strand at 2010.492 kb on - strand at 2010.494 kb on + strand at 2010.520 kb on - strand at 2010.525 kb on - strand at 2010.543 kb on + strand at 2010.575 kb on + strand at 2010.575 kb on + strand at 2010.576 kb on - strand at 2010.591 kb on - strand, within CA265_RS08090 at 2010.591 kb on - strand, within CA265_RS08090 at 2010.691 kb on - strand, within CA265_RS08090 at 2010.711 kb on - strand, within CA265_RS08090 at 2011.295 kb on + strand at 2011.295 kb on + strand at 2011.296 kb on - strand at 2011.296 kb on - strand at 2011.335 kb on - strand at 2011.450 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 2,008,521 - CA265_RS08075 0.50 -0.3 2,008,535 + CA265_RS08075 0.52 -0.1 2,008,787 + CA265_RS08075 0.77 -1.5 2,008,901 + CA265_RS08075 0.89 -0.4 2,008,902 - CA265_RS08075 0.89 -0.8 2,008,902 - CA265_RS08075 0.89 -0.1 2,008,908 + CA265_RS08075 0.89 -0.1 2,008,967 - +0.6 2,008,971 + -2.2 2,008,978 + +0.2 2,008,979 - -0.9 2,008,989 - -0.9 2,009,001 - +1.0 2,009,118 + CA265_RS08080 0.24 -0.6 2,009,145 - CA265_RS08080 0.31 -0.0 2,009,154 - CA265_RS08080 0.33 +1.0 2,009,235 + CA265_RS08080 0.53 -0.1 2,009,241 + CA265_RS08080 0.54 +0.1 2,009,294 + CA265_RS08080 0.67 -1.4 2,009,294 + CA265_RS08080 0.67 -0.2 2,009,343 - CA265_RS08080 0.79 +0.2 2,009,364 - CA265_RS08080 0.84 -0.4 2,009,364 - CA265_RS08080 0.84 +0.8 2,009,364 - CA265_RS08080 0.84 +0.3 2,009,368 - CA265_RS08080 0.85 -0.3 2,009,368 - CA265_RS08080 0.85 +1.5 2,009,368 - CA265_RS08080 0.85 +0.4 2,009,368 - CA265_RS08080 0.85 +0.2 2,009,413 - +0.5 2,009,422 - -0.1 2,009,429 - +1.2 2,009,433 - -0.9 2,009,436 + -0.7 2,009,545 + CA265_RS08085 0.11 -4.8 2,009,549 + CA265_RS08085 0.12 -2.9 2,009,550 - CA265_RS08085 0.12 -2.7 2,009,552 - CA265_RS08085 0.12 -1.0 2,009,595 - CA265_RS08085 0.16 -2.6 2,009,636 + CA265_RS08085 0.20 -1.8 2,009,636 + CA265_RS08085 0.20 -3.9 2,009,636 + CA265_RS08085 0.20 -3.6 2,009,637 - CA265_RS08085 0.20 -0.6 2,009,637 - CA265_RS08085 0.20 -1.5 2,009,638 + CA265_RS08085 0.20 -2.7 2,009,648 - CA265_RS08085 0.21 -2.7 2,009,648 - CA265_RS08085 0.21 -1.4 2,009,659 - CA265_RS08085 0.22 -0.6 2,009,733 + CA265_RS08085 0.29 -1.7 2,009,775 + CA265_RS08085 0.33 -3.3 2,009,836 - CA265_RS08085 0.39 -0.9 2,009,836 - CA265_RS08085 0.39 -1.4 2,009,836 - CA265_RS08085 0.39 -1.0 2,009,841 + CA265_RS08085 0.39 -2.6 2,009,857 - CA265_RS08085 0.41 -1.6 2,009,898 - CA265_RS08085 0.45 -0.6 2,009,903 - CA265_RS08085 0.45 -0.8 2,009,903 - CA265_RS08085 0.45 -1.9 2,009,999 + CA265_RS08085 0.54 -1.8 2,010,156 + CA265_RS08085 0.69 -2.9 2,010,161 + CA265_RS08085 0.70 -5.4 2,010,166 - CA265_RS08085 0.70 -1.6 2,010,334 - CA265_RS08085 0.86 -1.0 2,010,430 - -1.7 2,010,472 - +0.5 2,010,486 + -2.9 2,010,492 - +1.2 2,010,494 + -1.3 2,010,520 - -2.4 2,010,525 - -4.1 2,010,543 + -3.3 2,010,575 + -3.0 2,010,575 + -2.8 2,010,576 - -3.0 2,010,591 - CA265_RS08090 0.12 -4.8 2,010,591 - CA265_RS08090 0.12 -4.4 2,010,691 - CA265_RS08090 0.25 -4.6 2,010,711 - CA265_RS08090 0.27 -2.5 2,011,295 + -3.8 2,011,295 + -3.6 2,011,296 - +0.4 2,011,296 - -0.3 2,011,335 - +0.1 2,011,450 + -0.2
Or see this region's nucleotide sequence