Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS06195 and CA265_RS06200 are separated by 144 nucleotides CA265_RS06200 and CA265_RS06205 are separated by 234 nucleotides
CA265_RS06195: CA265_RS06195 - 3-octaprenyl-4-hydroxybenzoate carboxy-lyase, at 1,528,282 to 1,528,851
_RS06195
CA265_RS06200: CA265_RS06200 - tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))- methylthiotransferase MtaB, at 1,528,996 to 1,530,318
_RS06200
CA265_RS06205: CA265_RS06205 - hypothetical protein, at 1,530,553 to 1,531,551
_RS06205
Position (kb)
1528
1529
1530
1531 Strain fitness (log2 ratio)
-2
-1
0
1 at 1528.211 kb on + strand at 1528.212 kb on - strand at 1528.850 kb on - strand at 1528.920 kb on + strand at 1528.930 kb on + strand at 1528.930 kb on + strand at 1529.021 kb on + strand at 1529.022 kb on - strand at 1529.067 kb on - strand at 1529.119 kb on + strand at 1529.130 kb on + strand, within CA265_RS06200 at 1529.265 kb on - strand, within CA265_RS06200 at 1529.312 kb on + strand, within CA265_RS06200 at 1529.317 kb on + strand, within CA265_RS06200 at 1529.318 kb on - strand, within CA265_RS06200 at 1529.378 kb on + strand, within CA265_RS06200 at 1529.378 kb on + strand at 1529.385 kb on + strand, within CA265_RS06200 at 1529.425 kb on - strand, within CA265_RS06200 at 1529.456 kb on - strand, within CA265_RS06200 at 1529.464 kb on - strand, within CA265_RS06200 at 1529.464 kb on - strand, within CA265_RS06200 at 1529.660 kb on + strand, within CA265_RS06200 at 1529.683 kb on - strand, within CA265_RS06200 at 1529.865 kb on - strand, within CA265_RS06200 at 1529.888 kb on + strand, within CA265_RS06200 at 1529.895 kb on - strand, within CA265_RS06200 at 1529.941 kb on + strand, within CA265_RS06200 at 1529.942 kb on - strand, within CA265_RS06200 at 1529.942 kb on - strand, within CA265_RS06200 at 1529.981 kb on + strand, within CA265_RS06200 at 1529.985 kb on + strand, within CA265_RS06200 at 1530.006 kb on - strand, within CA265_RS06200 at 1530.064 kb on - strand, within CA265_RS06200 at 1530.087 kb on + strand, within CA265_RS06200 at 1530.211 kb on + strand at 1530.285 kb on - strand at 1530.357 kb on - strand at 1530.407 kb on + strand at 1530.422 kb on - strand at 1530.445 kb on + strand at 1530.446 kb on - strand at 1530.478 kb on - strand at 1530.478 kb on - strand at 1530.490 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 1,528,211 + +0.2 1,528,212 - +0.5 1,528,850 - +0.4 1,528,920 + -0.5 1,528,930 + +0.5 1,528,930 + -0.9 1,529,021 + -0.5 1,529,022 - -1.3 1,529,067 - -2.1 1,529,119 + -0.1 1,529,130 + CA265_RS06200 0.10 -1.3 1,529,265 - CA265_RS06200 0.20 -0.9 1,529,312 + CA265_RS06200 0.24 +1.1 1,529,317 + CA265_RS06200 0.24 -0.9 1,529,318 - CA265_RS06200 0.24 +1.4 1,529,378 + CA265_RS06200 0.29 -0.6 1,529,378 + -1.5 1,529,385 + CA265_RS06200 0.29 -0.1 1,529,425 - CA265_RS06200 0.32 -1.4 1,529,456 - CA265_RS06200 0.35 -1.4 1,529,464 - CA265_RS06200 0.35 -0.9 1,529,464 - CA265_RS06200 0.35 -2.1 1,529,660 + CA265_RS06200 0.50 -1.2 1,529,683 - CA265_RS06200 0.52 -1.2 1,529,865 - CA265_RS06200 0.66 -1.4 1,529,888 + CA265_RS06200 0.67 -1.3 1,529,895 - CA265_RS06200 0.68 -0.4 1,529,941 + CA265_RS06200 0.71 -0.0 1,529,942 - CA265_RS06200 0.72 +1.7 1,529,942 - CA265_RS06200 0.72 -0.3 1,529,981 + CA265_RS06200 0.74 -0.6 1,529,985 + CA265_RS06200 0.75 -1.0 1,530,006 - CA265_RS06200 0.76 -1.6 1,530,064 - CA265_RS06200 0.81 -1.4 1,530,087 + CA265_RS06200 0.82 +0.9 1,530,211 + -0.7 1,530,285 - -1.1 1,530,357 - -0.1 1,530,407 + +0.3 1,530,422 - +0.5 1,530,445 + -0.1 1,530,446 - -0.6 1,530,478 - +0.5 1,530,478 - +0.3 1,530,490 - -0.8
Or see this region's nucleotide sequence