Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04920 and CA265_RS04925 are separated by 44 nucleotides CA265_RS04925 and CA265_RS04930 are separated by 558 nucleotides CA265_RS04930 and CA265_RS04935 are separated by 69 nucleotides CA265_RS04935 and CA265_RS04940 are separated by 94 nucleotides
CA265_RS04920: CA265_RS04920 - hypothetical protein, at 1,173,491 to 1,174,918
_RS04920
CA265_RS04925: CA265_RS04925 - hypothetical protein, at 1,174,963 to 1,175,778
_RS04925
CA265_RS04930: CA265_RS04930 - tRNA-Leu, at 1,176,337 to 1,176,420
_RS04930
CA265_RS04935: CA265_RS04935 - tRNA-Gly, at 1,176,490 to 1,176,565
_RS04935
CA265_RS04940: CA265_RS04940 - RecX family transcriptional regulator, at 1,176,660 to 1,177,148
_RS04940
Position (kb)
1174
1175
1176 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1174.027 kb on + strand, within CA265_RS04920 at 1174.027 kb on + strand, within CA265_RS04920 at 1174.028 kb on - strand, within CA265_RS04920 at 1174.034 kb on - strand, within CA265_RS04920 at 1174.034 kb on - strand, within CA265_RS04920 at 1174.049 kb on - strand, within CA265_RS04920 at 1174.109 kb on + strand, within CA265_RS04920 at 1174.253 kb on + strand, within CA265_RS04920 at 1174.254 kb on - strand, within CA265_RS04920 at 1174.264 kb on - strand, within CA265_RS04920 at 1174.399 kb on - strand, within CA265_RS04920 at 1174.399 kb on - strand, within CA265_RS04920 at 1174.627 kb on + strand, within CA265_RS04920 at 1174.996 kb on - strand at 1174.996 kb on - strand at 1174.996 kb on - strand at 1175.008 kb on - strand at 1175.090 kb on + strand, within CA265_RS04925 at 1175.091 kb on - strand, within CA265_RS04925 at 1175.094 kb on - strand, within CA265_RS04925 at 1175.101 kb on - strand, within CA265_RS04925 at 1175.106 kb on - strand, within CA265_RS04925 at 1175.106 kb on - strand, within CA265_RS04925 at 1175.106 kb on - strand, within CA265_RS04925 at 1175.109 kb on - strand, within CA265_RS04925 at 1175.137 kb on + strand, within CA265_RS04925 at 1175.138 kb on - strand, within CA265_RS04925 at 1175.138 kb on - strand, within CA265_RS04925 at 1175.230 kb on - strand, within CA265_RS04925 at 1175.245 kb on - strand, within CA265_RS04925 at 1175.262 kb on + strand, within CA265_RS04925 at 1175.263 kb on - strand, within CA265_RS04925 at 1175.263 kb on - strand, within CA265_RS04925 at 1175.264 kb on + strand, within CA265_RS04925 at 1175.265 kb on - strand, within CA265_RS04925 at 1175.312 kb on - strand, within CA265_RS04925 at 1175.504 kb on + strand, within CA265_RS04925 at 1175.505 kb on - strand, within CA265_RS04925 at 1175.505 kb on - strand, within CA265_RS04925 at 1175.505 kb on - strand, within CA265_RS04925 at 1175.526 kb on + strand, within CA265_RS04925 at 1175.635 kb on + strand, within CA265_RS04925 at 1175.660 kb on + strand, within CA265_RS04925 at 1175.660 kb on + strand, within CA265_RS04925 at 1175.660 kb on + strand, within CA265_RS04925 at 1175.660 kb on + strand, within CA265_RS04925 at 1175.661 kb on - strand, within CA265_RS04925 at 1175.661 kb on - strand, within CA265_RS04925 at 1175.844 kb on - strand at 1175.910 kb on - strand at 1175.910 kb on - strand at 1176.082 kb on + strand at 1176.085 kb on - strand at 1176.126 kb on + strand at 1176.141 kb on - strand at 1176.142 kb on + strand at 1176.621 kb on - strand at 1176.691 kb on - strand at 1176.727 kb on + strand, within CA265_RS04940
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 1,174,027 + CA265_RS04920 0.38 -0.7 1,174,027 + CA265_RS04920 0.38 +0.0 1,174,028 - CA265_RS04920 0.38 -0.4 1,174,034 - CA265_RS04920 0.38 -0.3 1,174,034 - CA265_RS04920 0.38 +0.3 1,174,049 - CA265_RS04920 0.39 -1.1 1,174,109 + CA265_RS04920 0.43 -0.5 1,174,253 + CA265_RS04920 0.53 -0.2 1,174,254 - CA265_RS04920 0.53 -0.1 1,174,264 - CA265_RS04920 0.54 -0.8 1,174,399 - CA265_RS04920 0.64 -0.7 1,174,399 - CA265_RS04920 0.64 +0.6 1,174,627 + CA265_RS04920 0.80 +0.0 1,174,996 - -2.8 1,174,996 - -4.2 1,174,996 - -4.6 1,175,008 - -4.8 1,175,090 + CA265_RS04925 0.16 -0.8 1,175,091 - CA265_RS04925 0.16 -3.8 1,175,094 - CA265_RS04925 0.16 -4.6 1,175,101 - CA265_RS04925 0.17 -4.5 1,175,106 - CA265_RS04925 0.18 -4.2 1,175,106 - CA265_RS04925 0.18 -5.1 1,175,106 - CA265_RS04925 0.18 -3.2 1,175,109 - CA265_RS04925 0.18 -2.0 1,175,137 + CA265_RS04925 0.21 +0.5 1,175,138 - CA265_RS04925 0.21 -3.8 1,175,138 - CA265_RS04925 0.21 -4.9 1,175,230 - CA265_RS04925 0.33 -3.2 1,175,245 - CA265_RS04925 0.35 -1.1 1,175,262 + CA265_RS04925 0.37 -0.2 1,175,263 - CA265_RS04925 0.37 -4.3 1,175,263 - CA265_RS04925 0.37 -3.7 1,175,264 + CA265_RS04925 0.37 -2.5 1,175,265 - CA265_RS04925 0.37 -4.3 1,175,312 - CA265_RS04925 0.43 -3.4 1,175,504 + CA265_RS04925 0.66 -0.1 1,175,505 - CA265_RS04925 0.66 -3.7 1,175,505 - CA265_RS04925 0.66 -1.3 1,175,505 - CA265_RS04925 0.66 -4.1 1,175,526 + CA265_RS04925 0.69 +0.1 1,175,635 + CA265_RS04925 0.82 +0.6 1,175,660 + CA265_RS04925 0.85 -1.5 1,175,660 + CA265_RS04925 0.85 -0.9 1,175,660 + CA265_RS04925 0.85 +0.1 1,175,660 + CA265_RS04925 0.85 +0.4 1,175,661 - CA265_RS04925 0.86 -2.9 1,175,661 - CA265_RS04925 0.86 -1.7 1,175,844 - -1.4 1,175,910 - -3.6 1,175,910 - -4.4 1,176,082 + +0.2 1,176,085 - -3.4 1,176,126 + +0.2 1,176,141 - -3.1 1,176,142 + -0.5 1,176,621 - -0.5 1,176,691 - -0.6 1,176,727 + CA265_RS04940 0.14 +0.6
Or see this region's nucleotide sequence