Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04700 and CA265_RS04705 are separated by 15 nucleotides CA265_RS04705 and CA265_RS04710 are separated by 7 nucleotides CA265_RS04710 and CA265_RS04715 are separated by 26 nucleotides CA265_RS04715 and CA265_RS04720 are separated by 259 nucleotides CA265_RS04720 and CA265_RS04725 are separated by 99 nucleotides
CA265_RS04700: CA265_RS04700 - hypothetical protein, at 1,122,441 to 1,122,947
_RS04700
CA265_RS04705: CA265_RS04705 - hypothetical protein, at 1,122,963 to 1,123,382
_RS04705
CA265_RS04710: CA265_RS04710 - hypothetical protein, at 1,123,390 to 1,123,761
_RS04710
CA265_RS04715: CA265_RS04715 - VOC family protein, at 1,123,788 to 1,124,207
_RS04715
CA265_RS04720: CA265_RS04720 - DoxX family protein, at 1,124,467 to 1,124,844
_RS04720
CA265_RS04725: CA265_RS04725 - SIGNAL peptide protein, at 1,124,944 to 1,125,375
_RS04725
Position (kb)
1123
1124
1125 Strain fitness (log2 ratio)
-2
-1
0
1 at 1122.791 kb on + strand, within CA265_RS04700 at 1122.808 kb on + strand, within CA265_RS04700 at 1122.854 kb on - strand, within CA265_RS04700 at 1122.890 kb on + strand, within CA265_RS04700 at 1122.891 kb on - strand, within CA265_RS04700 at 1122.980 kb on + strand at 1122.980 kb on + strand at 1122.980 kb on + strand at 1123.004 kb on + strand at 1123.058 kb on + strand, within CA265_RS04705 at 1123.059 kb on - strand, within CA265_RS04705 at 1123.062 kb on + strand, within CA265_RS04705 at 1123.062 kb on + strand, within CA265_RS04705 at 1123.063 kb on - strand, within CA265_RS04705 at 1123.103 kb on - strand, within CA265_RS04705 at 1123.153 kb on - strand, within CA265_RS04705 at 1123.153 kb on - strand, within CA265_RS04705 at 1123.206 kb on - strand, within CA265_RS04705 at 1123.380 kb on + strand at 1123.381 kb on - strand at 1123.381 kb on - strand at 1123.411 kb on - strand at 1123.418 kb on - strand at 1123.444 kb on - strand, within CA265_RS04710 at 1123.531 kb on + strand, within CA265_RS04710 at 1123.535 kb on - strand, within CA265_RS04710 at 1123.537 kb on - strand, within CA265_RS04710 at 1123.576 kb on + strand, within CA265_RS04710 at 1123.576 kb on + strand, within CA265_RS04710 at 1123.580 kb on + strand, within CA265_RS04710 at 1123.752 kb on + strand at 1123.800 kb on - strand at 1124.016 kb on - strand, within CA265_RS04715 at 1124.016 kb on - strand, within CA265_RS04715 at 1124.089 kb on + strand, within CA265_RS04715 at 1124.090 kb on - strand, within CA265_RS04715 at 1124.156 kb on + strand, within CA265_RS04715 at 1124.191 kb on - strand at 1124.202 kb on - strand at 1124.291 kb on + strand at 1124.342 kb on - strand at 1124.359 kb on + strand at 1124.360 kb on - strand at 1124.371 kb on + strand at 1124.386 kb on + strand at 1124.389 kb on - strand at 1124.418 kb on - strand at 1124.429 kb on - strand at 1124.481 kb on + strand at 1124.512 kb on + strand, within CA265_RS04720 at 1124.513 kb on - strand, within CA265_RS04720 at 1124.655 kb on + strand, within CA265_RS04720 at 1124.656 kb on - strand, within CA265_RS04720 at 1124.656 kb on - strand, within CA265_RS04720 at 1124.657 kb on + strand, within CA265_RS04720 at 1124.658 kb on - strand, within CA265_RS04720 at 1124.661 kb on - strand, within CA265_RS04720 at 1124.667 kb on + strand, within CA265_RS04720 at 1124.667 kb on + strand, within CA265_RS04720 at 1124.787 kb on + strand, within CA265_RS04720 at 1124.822 kb on - strand at 1124.897 kb on + strand at 1124.930 kb on + strand at 1124.930 kb on + strand at 1124.931 kb on - strand at 1124.963 kb on + strand at 1125.018 kb on + strand, within CA265_RS04725 at 1125.083 kb on + strand, within CA265_RS04725 at 1125.085 kb on + strand, within CA265_RS04725 at 1125.086 kb on - strand, within CA265_RS04725 at 1125.088 kb on + strand, within CA265_RS04725 at 1125.141 kb on - strand, within CA265_RS04725 at 1125.141 kb on - strand, within CA265_RS04725 at 1125.198 kb on - strand, within CA265_RS04725
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 1,122,791 + CA265_RS04700 0.69 -2.0 1,122,808 + CA265_RS04700 0.72 +0.2 1,122,854 - CA265_RS04700 0.81 +0.6 1,122,890 + CA265_RS04700 0.89 -0.4 1,122,891 - CA265_RS04700 0.89 +1.5 1,122,980 + -0.0 1,122,980 + +0.7 1,122,980 + -0.2 1,123,004 + -0.7 1,123,058 + CA265_RS04705 0.23 -0.1 1,123,059 - CA265_RS04705 0.23 +0.3 1,123,062 + CA265_RS04705 0.24 -1.6 1,123,062 + CA265_RS04705 0.24 +0.4 1,123,063 - CA265_RS04705 0.24 +0.4 1,123,103 - CA265_RS04705 0.33 -2.6 1,123,153 - CA265_RS04705 0.45 -0.2 1,123,153 - CA265_RS04705 0.45 +0.2 1,123,206 - CA265_RS04705 0.58 +0.2 1,123,380 + -0.1 1,123,381 - -0.3 1,123,381 - +0.1 1,123,411 - +0.4 1,123,418 - -0.4 1,123,444 - CA265_RS04710 0.15 +0.3 1,123,531 + CA265_RS04710 0.38 +0.1 1,123,535 - CA265_RS04710 0.39 +0.7 1,123,537 - CA265_RS04710 0.40 -0.6 1,123,576 + CA265_RS04710 0.50 -0.4 1,123,576 + CA265_RS04710 0.50 +0.0 1,123,580 + CA265_RS04710 0.51 -0.2 1,123,752 + -0.3 1,123,800 - -0.3 1,124,016 - CA265_RS04715 0.54 -0.4 1,124,016 - CA265_RS04715 0.54 -0.7 1,124,089 + CA265_RS04715 0.72 -0.8 1,124,090 - CA265_RS04715 0.72 -1.6 1,124,156 + CA265_RS04715 0.88 -0.0 1,124,191 - +0.5 1,124,202 - -1.0 1,124,291 + -1.0 1,124,342 - -0.1 1,124,359 + -0.1 1,124,360 - -0.7 1,124,371 + -0.0 1,124,386 + -0.2 1,124,389 - -0.2 1,124,418 - -0.4 1,124,429 - +0.3 1,124,481 + +0.2 1,124,512 + CA265_RS04720 0.12 -0.4 1,124,513 - CA265_RS04720 0.12 -0.1 1,124,655 + CA265_RS04720 0.50 -0.3 1,124,656 - CA265_RS04720 0.50 -0.3 1,124,656 - CA265_RS04720 0.50 -0.1 1,124,657 + CA265_RS04720 0.50 +1.3 1,124,658 - CA265_RS04720 0.51 -0.4 1,124,661 - CA265_RS04720 0.51 -0.7 1,124,667 + CA265_RS04720 0.53 -0.5 1,124,667 + CA265_RS04720 0.53 -0.6 1,124,787 + CA265_RS04720 0.85 -0.4 1,124,822 - +0.2 1,124,897 + +0.2 1,124,930 + -0.3 1,124,930 + +0.2 1,124,931 - +0.6 1,124,963 + -0.2 1,125,018 + CA265_RS04725 0.17 -1.0 1,125,083 + CA265_RS04725 0.32 +0.0 1,125,085 + CA265_RS04725 0.33 +0.2 1,125,086 - CA265_RS04725 0.33 +0.2 1,125,088 + CA265_RS04725 0.33 +1.0 1,125,141 - CA265_RS04725 0.46 +0.5 1,125,141 - CA265_RS04725 0.46 +0.4 1,125,198 - CA265_RS04725 0.59 -0.2
Or see this region's nucleotide sequence