Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04405 and CA265_RS04410 are separated by 41 nucleotides CA265_RS04410 and CA265_RS04415 are separated by 196 nucleotides CA265_RS04415 and CA265_RS04420 are separated by 40 nucleotides CA265_RS04420 and CA265_RS04425 are separated by 19 nucleotides
CA265_RS04405: CA265_RS04405 - DNA polymerase/3'-5' exonuclease PolX, at 1,056,687 to 1,058,363
_RS04405
CA265_RS04410: CA265_RS04410 - hypothetical protein, at 1,058,405 to 1,059,082
_RS04410
CA265_RS04415: CA265_RS04415 - signal peptide peptidase SppA, at 1,059,279 to 1,061,045
_RS04415
CA265_RS04420: CA265_RS04420 - 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine diphosphokinase, at 1,061,086 to 1,061,592
_RS04420
CA265_RS04425: CA265_RS04425 - histidine phosphotransferase, at 1,061,612 to 1,061,980
_RS04425
Position (kb)
1059
1060
1061
1062 Strain fitness (log2 ratio)
-2
-1
0
1 at 1058.315 kb on + strand at 1058.326 kb on - strand at 1058.384 kb on - strand at 1058.417 kb on - strand at 1058.446 kb on + strand at 1058.488 kb on + strand, within CA265_RS04410 at 1058.491 kb on - strand, within CA265_RS04410 at 1058.491 kb on - strand, within CA265_RS04410 at 1058.491 kb on - strand, within CA265_RS04410 at 1058.642 kb on - strand, within CA265_RS04410 at 1058.717 kb on + strand, within CA265_RS04410 at 1058.761 kb on - strand, within CA265_RS04410 at 1058.762 kb on + strand, within CA265_RS04410 at 1058.785 kb on + strand, within CA265_RS04410 at 1058.785 kb on + strand, within CA265_RS04410 at 1058.824 kb on + strand, within CA265_RS04410 at 1058.949 kb on - strand, within CA265_RS04410 at 1059.114 kb on - strand at 1059.114 kb on - strand at 1059.142 kb on - strand at 1059.188 kb on - strand at 1059.220 kb on + strand at 1059.220 kb on + strand at 1059.233 kb on - strand at 1059.233 kb on - strand at 1059.233 kb on - strand at 1059.252 kb on - strand at 1059.288 kb on - strand at 1059.361 kb on + strand at 1059.362 kb on - strand at 1059.386 kb on + strand at 1059.386 kb on + strand at 1059.387 kb on - strand at 1059.387 kb on - strand at 1059.388 kb on + strand at 1059.388 kb on + strand at 1059.391 kb on + strand at 1059.392 kb on - strand at 1059.399 kb on - strand at 1059.444 kb on + strand at 1059.445 kb on - strand at 1059.459 kb on + strand, within CA265_RS04415 at 1059.590 kb on + strand, within CA265_RS04415 at 1059.590 kb on + strand, within CA265_RS04415 at 1059.599 kb on - strand, within CA265_RS04415 at 1059.650 kb on - strand, within CA265_RS04415 at 1059.675 kb on - strand, within CA265_RS04415 at 1059.716 kb on + strand, within CA265_RS04415 at 1059.720 kb on - strand, within CA265_RS04415 at 1059.728 kb on - strand, within CA265_RS04415 at 1059.740 kb on + strand, within CA265_RS04415 at 1059.755 kb on + strand, within CA265_RS04415 at 1059.756 kb on - strand, within CA265_RS04415 at 1059.763 kb on - strand, within CA265_RS04415 at 1059.824 kb on + strand, within CA265_RS04415 at 1059.869 kb on + strand, within CA265_RS04415 at 1059.870 kb on - strand, within CA265_RS04415 at 1059.911 kb on + strand, within CA265_RS04415 at 1059.936 kb on + strand, within CA265_RS04415 at 1059.937 kb on - strand, within CA265_RS04415 at 1060.052 kb on + strand, within CA265_RS04415 at 1060.052 kb on + strand, within CA265_RS04415 at 1060.053 kb on - strand, within CA265_RS04415 at 1060.094 kb on + strand, within CA265_RS04415 at 1060.112 kb on - strand, within CA265_RS04415 at 1060.224 kb on + strand, within CA265_RS04415 at 1060.224 kb on + strand, within CA265_RS04415 at 1060.331 kb on + strand, within CA265_RS04415 at 1060.332 kb on - strand, within CA265_RS04415 at 1060.377 kb on - strand, within CA265_RS04415 at 1060.393 kb on - strand, within CA265_RS04415 at 1060.396 kb on + strand, within CA265_RS04415 at 1060.397 kb on - strand, within CA265_RS04415 at 1060.420 kb on + strand, within CA265_RS04415 at 1060.531 kb on - strand, within CA265_RS04415 at 1060.531 kb on - strand, within CA265_RS04415 at 1060.607 kb on - strand, within CA265_RS04415 at 1060.626 kb on + strand, within CA265_RS04415 at 1060.636 kb on + strand, within CA265_RS04415 at 1060.726 kb on - strand, within CA265_RS04415 at 1060.726 kb on - strand, within CA265_RS04415 at 1060.735 kb on + strand, within CA265_RS04415 at 1060.739 kb on + strand, within CA265_RS04415 at 1060.739 kb on + strand, within CA265_RS04415 at 1060.740 kb on - strand, within CA265_RS04415 at 1060.748 kb on + strand, within CA265_RS04415 at 1060.752 kb on - strand, within CA265_RS04415 at 1060.795 kb on - strand, within CA265_RS04415 at 1060.801 kb on - strand, within CA265_RS04415 at 1060.860 kb on - strand, within CA265_RS04415 at 1060.860 kb on - strand, within CA265_RS04415 at 1060.896 kb on + strand at 1060.904 kb on + strand at 1060.963 kb on - strand at 1060.980 kb on + strand at 1060.987 kb on - strand at 1061.012 kb on + strand at 1061.013 kb on - strand at 1061.611 kb on + strand at 1061.733 kb on - strand, within CA265_RS04425 at 1061.772 kb on + strand, within CA265_RS04425 at 1061.776 kb on + strand, within CA265_RS04425 at 1061.811 kb on - strand, within CA265_RS04425 at 1061.811 kb on - strand, within CA265_RS04425 at 1061.812 kb on + strand, within CA265_RS04425 at 1061.815 kb on - strand, within CA265_RS04425 at 1061.930 kb on - strand, within CA265_RS04425 at 1061.930 kb on - strand, within CA265_RS04425 at 1062.016 kb on - strand at 1062.020 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 1,058,315 + -0.1 1,058,326 - +0.0 1,058,384 - +0.0 1,058,417 - +0.2 1,058,446 + -0.9 1,058,488 + CA265_RS04410 0.12 -0.1 1,058,491 - CA265_RS04410 0.13 -1.0 1,058,491 - CA265_RS04410 0.13 -0.1 1,058,491 - CA265_RS04410 0.13 -0.2 1,058,642 - CA265_RS04410 0.35 +0.6 1,058,717 + CA265_RS04410 0.46 -0.6 1,058,761 - CA265_RS04410 0.53 -0.3 1,058,762 + CA265_RS04410 0.53 -0.3 1,058,785 + CA265_RS04410 0.56 -1.0 1,058,785 + CA265_RS04410 0.56 +0.9 1,058,824 + CA265_RS04410 0.62 +0.0 1,058,949 - CA265_RS04410 0.80 -0.1 1,059,114 - +0.3 1,059,114 - -1.1 1,059,142 - -0.2 1,059,188 - -0.5 1,059,220 + +0.3 1,059,220 + -0.6 1,059,233 - -0.1 1,059,233 - -0.1 1,059,233 - -0.6 1,059,252 - -0.1 1,059,288 - -1.5 1,059,361 + -0.6 1,059,362 - -0.5 1,059,386 + +0.1 1,059,386 + +0.2 1,059,387 - -1.2 1,059,387 - -1.4 1,059,388 + -0.9 1,059,388 + -1.1 1,059,391 + -0.3 1,059,392 - +0.2 1,059,399 - -0.4 1,059,444 + -1.8 1,059,445 - -0.9 1,059,459 + CA265_RS04415 0.10 -0.7 1,059,590 + CA265_RS04415 0.18 -1.6 1,059,590 + CA265_RS04415 0.18 +0.6 1,059,599 - CA265_RS04415 0.18 -0.6 1,059,650 - CA265_RS04415 0.21 -1.3 1,059,675 - CA265_RS04415 0.22 -1.6 1,059,716 + CA265_RS04415 0.25 -0.8 1,059,720 - CA265_RS04415 0.25 -1.1 1,059,728 - CA265_RS04415 0.25 -1.6 1,059,740 + CA265_RS04415 0.26 -0.3 1,059,755 + CA265_RS04415 0.27 -1.0 1,059,756 - CA265_RS04415 0.27 -0.6 1,059,763 - CA265_RS04415 0.27 -2.4 1,059,824 + CA265_RS04415 0.31 +0.2 1,059,869 + CA265_RS04415 0.33 -0.7 1,059,870 - CA265_RS04415 0.33 -1.2 1,059,911 + CA265_RS04415 0.36 -1.4 1,059,936 + CA265_RS04415 0.37 -1.5 1,059,937 - CA265_RS04415 0.37 -2.3 1,060,052 + CA265_RS04415 0.44 -0.8 1,060,052 + CA265_RS04415 0.44 -1.1 1,060,053 - CA265_RS04415 0.44 -1.6 1,060,094 + CA265_RS04415 0.46 -1.9 1,060,112 - CA265_RS04415 0.47 -1.9 1,060,224 + CA265_RS04415 0.53 +0.1 1,060,224 + CA265_RS04415 0.53 -1.3 1,060,331 + CA265_RS04415 0.60 -0.6 1,060,332 - CA265_RS04415 0.60 -2.1 1,060,377 - CA265_RS04415 0.62 -0.2 1,060,393 - CA265_RS04415 0.63 +0.2 1,060,396 + CA265_RS04415 0.63 -1.1 1,060,397 - CA265_RS04415 0.63 -0.9 1,060,420 + CA265_RS04415 0.65 +0.1 1,060,531 - CA265_RS04415 0.71 -1.7 1,060,531 - CA265_RS04415 0.71 -0.9 1,060,607 - CA265_RS04415 0.75 -2.0 1,060,626 + CA265_RS04415 0.76 -0.9 1,060,636 + CA265_RS04415 0.77 -1.1 1,060,726 - CA265_RS04415 0.82 -0.5 1,060,726 - CA265_RS04415 0.82 -2.2 1,060,735 + CA265_RS04415 0.82 -0.6 1,060,739 + CA265_RS04415 0.83 -0.6 1,060,739 + CA265_RS04415 0.83 -0.7 1,060,740 - CA265_RS04415 0.83 -0.4 1,060,748 + CA265_RS04415 0.83 -0.8 1,060,752 - CA265_RS04415 0.83 -1.5 1,060,795 - CA265_RS04415 0.86 -0.5 1,060,801 - CA265_RS04415 0.86 -1.7 1,060,860 - CA265_RS04415 0.89 -1.8 1,060,860 - CA265_RS04415 0.89 -2.1 1,060,896 + -1.2 1,060,904 + -2.1 1,060,963 - -1.1 1,060,980 + -0.6 1,060,987 - -1.2 1,061,012 + -0.5 1,061,013 - -1.6 1,061,611 + +1.1 1,061,733 - CA265_RS04425 0.33 +0.6 1,061,772 + CA265_RS04425 0.43 +0.8 1,061,776 + CA265_RS04425 0.44 +0.2 1,061,811 - CA265_RS04425 0.54 +0.5 1,061,811 - CA265_RS04425 0.54 +0.4 1,061,812 + CA265_RS04425 0.54 -1.0 1,061,815 - CA265_RS04425 0.55 +0.2 1,061,930 - CA265_RS04425 0.86 +0.6 1,061,930 - CA265_RS04425 0.86 +0.1 1,062,016 - +1.5 1,062,020 - -0.6
Or see this region's nucleotide sequence