Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04265 and CA265_RS04270 are separated by 157 nucleotides CA265_RS04270 and CA265_RS04275 overlap by 17 nucleotides CA265_RS04275 and CA265_RS04280 are separated by 152 nucleotides
CA265_RS04265: CA265_RS04265 - hypothetical protein, at 1,032,661 to 1,033,017
_RS04265
CA265_RS04270: CA265_RS04270 - ATP-dependent Clp protease proteolytic subunit, at 1,033,175 to 1,033,882
_RS04270
CA265_RS04275: CA265_RS04275 - ATP-dependent protease ATP-binding subunit ClpX, at 1,033,866 to 1,035,104
_RS04275
CA265_RS04280: CA265_RS04280 - AMP nucleosidase, at 1,035,257 to 1,036,063
_RS04280
Position (kb)
1033
1034
1035
1036 Strain fitness (log2 ratio)
-2
-1
0
1 at 1032.957 kb on - strand, within CA265_RS04265 at 1032.978 kb on - strand, within CA265_RS04265 at 1033.053 kb on - strand at 1033.078 kb on - strand at 1033.111 kb on + strand at 1034.040 kb on - strand, within CA265_RS04275 at 1034.041 kb on + strand, within CA265_RS04275 at 1034.085 kb on + strand, within CA265_RS04275 at 1034.086 kb on - strand, within CA265_RS04275 at 1034.120 kb on - strand, within CA265_RS04275 at 1034.120 kb on - strand, within CA265_RS04275 at 1034.169 kb on - strand, within CA265_RS04275 at 1034.341 kb on + strand, within CA265_RS04275 at 1034.398 kb on + strand, within CA265_RS04275 at 1034.399 kb on - strand, within CA265_RS04275 at 1034.399 kb on - strand, within CA265_RS04275 at 1034.403 kb on - strand, within CA265_RS04275 at 1034.418 kb on - strand, within CA265_RS04275 at 1034.448 kb on - strand, within CA265_RS04275 at 1034.507 kb on + strand, within CA265_RS04275 at 1034.515 kb on + strand, within CA265_RS04275 at 1034.516 kb on - strand, within CA265_RS04275 at 1034.582 kb on + strand, within CA265_RS04275 at 1034.583 kb on - strand, within CA265_RS04275 at 1034.583 kb on - strand, within CA265_RS04275 at 1034.587 kb on + strand, within CA265_RS04275 at 1034.588 kb on - strand, within CA265_RS04275 at 1034.588 kb on - strand, within CA265_RS04275 at 1034.592 kb on - strand, within CA265_RS04275 at 1034.645 kb on + strand, within CA265_RS04275 at 1034.713 kb on - strand, within CA265_RS04275 at 1034.714 kb on + strand, within CA265_RS04275 at 1034.715 kb on - strand, within CA265_RS04275 at 1034.715 kb on - strand, within CA265_RS04275 at 1034.802 kb on - strand, within CA265_RS04275 at 1034.856 kb on + strand, within CA265_RS04275 at 1034.857 kb on - strand, within CA265_RS04275 at 1034.942 kb on + strand, within CA265_RS04275 at 1035.006 kb on - strand at 1035.148 kb on - strand at 1035.200 kb on - strand at 1035.268 kb on - strand at 1035.334 kb on + strand at 1035.335 kb on - strand at 1035.349 kb on - strand, within CA265_RS04280 at 1035.365 kb on + strand, within CA265_RS04280 at 1035.366 kb on - strand, within CA265_RS04280 at 1035.368 kb on - strand, within CA265_RS04280 at 1035.389 kb on - strand, within CA265_RS04280 at 1035.389 kb on - strand, within CA265_RS04280 at 1035.392 kb on + strand, within CA265_RS04280 at 1035.392 kb on + strand, within CA265_RS04280 at 1035.397 kb on - strand, within CA265_RS04280 at 1035.531 kb on + strand, within CA265_RS04280 at 1035.532 kb on - strand, within CA265_RS04280 at 1035.613 kb on - strand, within CA265_RS04280 at 1035.671 kb on + strand, within CA265_RS04280 at 1035.715 kb on + strand, within CA265_RS04280 at 1035.822 kb on + strand, within CA265_RS04280 at 1035.837 kb on + strand, within CA265_RS04280 at 1035.837 kb on + strand, within CA265_RS04280 at 1035.838 kb on - strand, within CA265_RS04280 at 1035.841 kb on + strand, within CA265_RS04280 at 1035.940 kb on + strand, within CA265_RS04280 at 1035.993 kb on + strand at 1035.993 kb on + strand at 1036.094 kb on - strand at 1036.096 kb on + strand at 1036.103 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 1,032,957 - CA265_RS04265 0.83 +0.8 1,032,978 - CA265_RS04265 0.89 -0.1 1,033,053 - -0.6 1,033,078 - -1.3 1,033,111 + -1.4 1,034,040 - CA265_RS04275 0.14 -0.6 1,034,041 + CA265_RS04275 0.14 -2.6 1,034,085 + CA265_RS04275 0.18 -0.2 1,034,086 - CA265_RS04275 0.18 -0.8 1,034,120 - CA265_RS04275 0.21 -0.1 1,034,120 - CA265_RS04275 0.21 -0.9 1,034,169 - CA265_RS04275 0.24 +0.0 1,034,341 + CA265_RS04275 0.38 -1.1 1,034,398 + CA265_RS04275 0.43 -1.2 1,034,399 - CA265_RS04275 0.43 -0.3 1,034,399 - CA265_RS04275 0.43 -0.1 1,034,403 - CA265_RS04275 0.43 -1.3 1,034,418 - CA265_RS04275 0.45 -0.8 1,034,448 - CA265_RS04275 0.47 -0.3 1,034,507 + CA265_RS04275 0.52 -1.2 1,034,515 + CA265_RS04275 0.52 -0.9 1,034,516 - CA265_RS04275 0.52 -0.5 1,034,582 + CA265_RS04275 0.58 -0.6 1,034,583 - CA265_RS04275 0.58 -1.0 1,034,583 - CA265_RS04275 0.58 +0.3 1,034,587 + CA265_RS04275 0.58 -1.3 1,034,588 - CA265_RS04275 0.58 -1.4 1,034,588 - CA265_RS04275 0.58 +0.0 1,034,592 - CA265_RS04275 0.59 -0.1 1,034,645 + CA265_RS04275 0.63 -0.7 1,034,713 - CA265_RS04275 0.68 -0.5 1,034,714 + CA265_RS04275 0.68 -0.9 1,034,715 - CA265_RS04275 0.69 -0.8 1,034,715 - CA265_RS04275 0.69 -0.9 1,034,802 - CA265_RS04275 0.76 +0.8 1,034,856 + CA265_RS04275 0.80 -1.6 1,034,857 - CA265_RS04275 0.80 -0.2 1,034,942 + CA265_RS04275 0.87 -0.8 1,035,006 - -0.0 1,035,148 - -0.4 1,035,200 - -0.4 1,035,268 - +0.7 1,035,334 + +0.2 1,035,335 - -0.3 1,035,349 - CA265_RS04280 0.11 -0.2 1,035,365 + CA265_RS04280 0.13 -1.6 1,035,366 - CA265_RS04280 0.14 +1.6 1,035,368 - CA265_RS04280 0.14 -0.4 1,035,389 - CA265_RS04280 0.16 +0.4 1,035,389 - CA265_RS04280 0.16 +0.1 1,035,392 + CA265_RS04280 0.17 +1.1 1,035,392 + CA265_RS04280 0.17 -0.4 1,035,397 - CA265_RS04280 0.17 -0.0 1,035,531 + CA265_RS04280 0.34 -0.5 1,035,532 - CA265_RS04280 0.34 -0.4 1,035,613 - CA265_RS04280 0.44 -2.3 1,035,671 + CA265_RS04280 0.51 +0.0 1,035,715 + CA265_RS04280 0.57 -0.2 1,035,822 + CA265_RS04280 0.70 -0.0 1,035,837 + CA265_RS04280 0.72 -0.7 1,035,837 + CA265_RS04280 0.72 -0.5 1,035,838 - CA265_RS04280 0.72 +0.4 1,035,841 + CA265_RS04280 0.72 -0.5 1,035,940 + CA265_RS04280 0.85 -0.4 1,035,993 + +0.6 1,035,993 + +0.4 1,036,094 - +0.0 1,036,096 + -0.1 1,036,103 + -0.4
Or see this region's nucleotide sequence