Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03850 and CA265_RS03855 are separated by 2 nucleotides CA265_RS03855 and CA265_RS03860 are separated by 328 nucleotides
CA265_RS03850: CA265_RS03850 - peptidase C69, at 906,378 to 907,694
_RS03850
CA265_RS03855: CA265_RS03855 - TldD protein, at 907,697 to 909,331
_RS03855
CA265_RS03860: CA265_RS03860 - hypothetical protein, at 909,660 to 911,444
_RS03860
Position (kb)
907
908
909
910 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 906.783 kb on + strand, within CA265_RS03850 at 906.982 kb on - strand, within CA265_RS03850 at 907.022 kb on - strand, within CA265_RS03850 at 907.022 kb on - strand, within CA265_RS03850 at 907.097 kb on + strand, within CA265_RS03850 at 907.110 kb on + strand, within CA265_RS03850 at 907.157 kb on + strand, within CA265_RS03850 at 907.157 kb on + strand, within CA265_RS03850 at 907.191 kb on - strand, within CA265_RS03850 at 907.201 kb on + strand, within CA265_RS03850 at 907.201 kb on + strand, within CA265_RS03850 at 907.236 kb on - strand, within CA265_RS03850 at 907.290 kb on + strand, within CA265_RS03850 at 907.357 kb on + strand, within CA265_RS03850 at 907.357 kb on + strand, within CA265_RS03850 at 907.357 kb on + strand, within CA265_RS03850 at 907.358 kb on - strand, within CA265_RS03850 at 907.358 kb on - strand, within CA265_RS03850 at 907.389 kb on + strand, within CA265_RS03850 at 907.401 kb on - strand, within CA265_RS03850 at 907.524 kb on + strand, within CA265_RS03850 at 907.586 kb on + strand at 907.614 kb on + strand at 907.615 kb on - strand at 907.683 kb on + strand at 907.684 kb on - strand at 907.684 kb on - strand at 907.684 kb on - strand at 907.687 kb on - strand at 907.694 kb on + strand at 907.714 kb on + strand at 907.881 kb on + strand, within CA265_RS03855 at 908.001 kb on + strand, within CA265_RS03855 at 908.030 kb on - strand, within CA265_RS03855 at 908.176 kb on - strand, within CA265_RS03855 at 908.284 kb on + strand, within CA265_RS03855 at 908.358 kb on + strand, within CA265_RS03855 at 908.375 kb on + strand, within CA265_RS03855 at 908.387 kb on + strand, within CA265_RS03855 at 908.444 kb on + strand, within CA265_RS03855 at 908.458 kb on - strand, within CA265_RS03855 at 908.550 kb on + strand, within CA265_RS03855 at 908.596 kb on + strand, within CA265_RS03855 at 908.596 kb on + strand, within CA265_RS03855 at 908.597 kb on - strand, within CA265_RS03855 at 908.597 kb on - strand, within CA265_RS03855 at 908.598 kb on + strand, within CA265_RS03855 at 908.650 kb on + strand, within CA265_RS03855 at 908.651 kb on - strand, within CA265_RS03855 at 908.680 kb on + strand, within CA265_RS03855 at 908.719 kb on - strand, within CA265_RS03855 at 908.732 kb on - strand, within CA265_RS03855 at 908.742 kb on + strand, within CA265_RS03855 at 908.888 kb on + strand, within CA265_RS03855 at 908.889 kb on - strand, within CA265_RS03855 at 908.899 kb on - strand, within CA265_RS03855 at 908.937 kb on - strand, within CA265_RS03855 at 908.937 kb on - strand, within CA265_RS03855 at 908.941 kb on + strand, within CA265_RS03855 at 908.943 kb on - strand, within CA265_RS03855 at 909.052 kb on + strand, within CA265_RS03855 at 909.063 kb on + strand, within CA265_RS03855 at 909.063 kb on + strand, within CA265_RS03855 at 909.064 kb on - strand, within CA265_RS03855 at 909.111 kb on - strand, within CA265_RS03855 at 909.150 kb on + strand, within CA265_RS03855 at 909.212 kb on + strand at 909.246 kb on - strand at 909.429 kb on - strand at 909.432 kb on + strand at 909.444 kb on - strand at 909.545 kb on + strand at 909.685 kb on + strand at 909.738 kb on + strand at 909.785 kb on + strand at 909.786 kb on - strand at 909.871 kb on + strand, within CA265_RS03860 at 909.872 kb on - strand, within CA265_RS03860 at 909.872 kb on - strand, within CA265_RS03860 at 909.931 kb on + strand, within CA265_RS03860 at 909.933 kb on + strand, within CA265_RS03860 at 909.933 kb on + strand, within CA265_RS03860 at 909.934 kb on - strand, within CA265_RS03860 at 910.038 kb on - strand, within CA265_RS03860 at 910.092 kb on - strand, within CA265_RS03860 at 910.138 kb on + strand, within CA265_RS03860 at 910.138 kb on + strand, within CA265_RS03860 at 910.148 kb on - strand, within CA265_RS03860 at 910.167 kb on + strand, within CA265_RS03860 at 910.180 kb on + strand, within CA265_RS03860 at 910.180 kb on + strand, within CA265_RS03860 at 910.184 kb on + strand, within CA265_RS03860 at 910.196 kb on + strand, within CA265_RS03860 at 910.196 kb on + strand, within CA265_RS03860 at 910.197 kb on - strand, within CA265_RS03860 at 910.207 kb on - strand, within CA265_RS03860 at 910.233 kb on - strand, within CA265_RS03860 at 910.265 kb on + strand, within CA265_RS03860 at 910.265 kb on + strand, within CA265_RS03860 at 910.266 kb on - strand, within CA265_RS03860 at 910.266 kb on - strand, within CA265_RS03860
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 906,783 + CA265_RS03850 0.31 +0.7 906,982 - CA265_RS03850 0.46 -0.4 907,022 - CA265_RS03850 0.49 -0.7 907,022 - CA265_RS03850 0.49 -0.7 907,097 + CA265_RS03850 0.55 +0.3 907,110 + CA265_RS03850 0.56 -0.5 907,157 + CA265_RS03850 0.59 -0.2 907,157 + CA265_RS03850 0.59 -0.1 907,191 - CA265_RS03850 0.62 -0.3 907,201 + CA265_RS03850 0.62 +0.0 907,201 + CA265_RS03850 0.62 -0.9 907,236 - CA265_RS03850 0.65 -0.6 907,290 + CA265_RS03850 0.69 -1.0 907,357 + CA265_RS03850 0.74 -0.1 907,357 + CA265_RS03850 0.74 +0.2 907,357 + CA265_RS03850 0.74 -0.6 907,358 - CA265_RS03850 0.74 -1.3 907,358 - CA265_RS03850 0.74 -0.5 907,389 + CA265_RS03850 0.77 +0.5 907,401 - CA265_RS03850 0.78 -1.4 907,524 + CA265_RS03850 0.87 -1.5 907,586 + -0.4 907,614 + +0.2 907,615 - -0.3 907,683 + -0.1 907,684 - +0.4 907,684 - +0.4 907,684 - +0.8 907,687 - -1.3 907,694 + -0.5 907,714 + +0.2 907,881 + CA265_RS03855 0.11 -2.0 908,001 + CA265_RS03855 0.19 +0.1 908,030 - CA265_RS03855 0.20 -0.8 908,176 - CA265_RS03855 0.29 -0.6 908,284 + CA265_RS03855 0.36 -0.9 908,358 + CA265_RS03855 0.40 -0.3 908,375 + CA265_RS03855 0.41 -0.3 908,387 + CA265_RS03855 0.42 -1.3 908,444 + CA265_RS03855 0.46 -0.1 908,458 - CA265_RS03855 0.47 -0.6 908,550 + CA265_RS03855 0.52 -0.0 908,596 + CA265_RS03855 0.55 +0.1 908,596 + CA265_RS03855 0.55 +0.9 908,597 - CA265_RS03855 0.55 +0.3 908,597 - CA265_RS03855 0.55 -0.8 908,598 + CA265_RS03855 0.55 -0.0 908,650 + CA265_RS03855 0.58 +0.0 908,651 - CA265_RS03855 0.58 -0.3 908,680 + CA265_RS03855 0.60 +0.7 908,719 - CA265_RS03855 0.63 -0.1 908,732 - CA265_RS03855 0.63 -0.0 908,742 + CA265_RS03855 0.64 -0.6 908,888 + CA265_RS03855 0.73 -1.0 908,889 - CA265_RS03855 0.73 +1.0 908,899 - CA265_RS03855 0.74 -0.4 908,937 - CA265_RS03855 0.76 +0.2 908,937 - CA265_RS03855 0.76 -0.5 908,941 + CA265_RS03855 0.76 -0.2 908,943 - CA265_RS03855 0.76 -0.1 909,052 + CA265_RS03855 0.83 -0.0 909,063 + CA265_RS03855 0.84 -0.7 909,063 + CA265_RS03855 0.84 -0.9 909,064 - CA265_RS03855 0.84 -0.3 909,111 - CA265_RS03855 0.86 -0.7 909,150 + CA265_RS03855 0.89 +1.0 909,212 + +0.4 909,246 - +0.3 909,429 - -0.9 909,432 + -0.5 909,444 - +0.3 909,545 + -0.1 909,685 + -0.7 909,738 + -1.3 909,785 + -0.0 909,786 - -0.3 909,871 + CA265_RS03860 0.12 -0.4 909,872 - CA265_RS03860 0.12 -0.6 909,872 - CA265_RS03860 0.12 +0.1 909,931 + CA265_RS03860 0.15 +0.3 909,933 + CA265_RS03860 0.15 -0.5 909,933 + CA265_RS03860 0.15 -0.9 909,934 - CA265_RS03860 0.15 +0.6 910,038 - CA265_RS03860 0.21 +0.9 910,092 - CA265_RS03860 0.24 +0.4 910,138 + CA265_RS03860 0.27 +0.0 910,138 + CA265_RS03860 0.27 +2.3 910,148 - CA265_RS03860 0.27 -0.6 910,167 + CA265_RS03860 0.28 +0.2 910,180 + CA265_RS03860 0.29 -0.1 910,180 + CA265_RS03860 0.29 +0.2 910,184 + CA265_RS03860 0.29 -0.6 910,196 + CA265_RS03860 0.30 +0.1 910,196 + CA265_RS03860 0.30 +1.0 910,197 - CA265_RS03860 0.30 -0.2 910,207 - CA265_RS03860 0.31 +0.5 910,233 - CA265_RS03860 0.32 +0.5 910,265 + CA265_RS03860 0.34 +0.1 910,265 + CA265_RS03860 0.34 +0.1 910,266 - CA265_RS03860 0.34 +0.6 910,266 - CA265_RS03860 0.34 -0.3
Or see this region's nucleotide sequence