Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03780 and CA265_RS03785 are separated by 179 nucleotides CA265_RS03785 and CA265_RS03790 are separated by 82 nucleotides CA265_RS03790 and CA265_RS03795 overlap by 4 nucleotides CA265_RS03795 and CA265_RS03800 are separated by 387 nucleotides
CA265_RS03780: CA265_RS03780 - transcriptional regulator, at 895,580 to 896,470
_RS03780
CA265_RS03785: CA265_RS03785 - hypothetical protein, at 896,650 to 897,189
_RS03785
CA265_RS03790: CA265_RS03790 - hypothetical protein, at 897,272 to 897,769
_RS03790
CA265_RS03795: CA265_RS03795 - hypothetical protein, at 897,766 to 898,158
_RS03795
CA265_RS03800: CA265_RS03800 - TetR family transcriptional regulator, at 898,546 to 899,169
_RS03800
Position (kb) 897
898 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 896.287 kb on + strand, within CA265_RS03780 at 896.396 kb on + strand at 896.397 kb on - strand at 896.404 kb on + strand at 896.404 kb on + strand at 896.405 kb on - strand at 896.405 kb on - strand at 896.430 kb on + strand at 896.430 kb on + strand at 896.430 kb on + strand at 896.450 kb on + strand at 896.511 kb on - strand at 896.635 kb on - strand at 896.665 kb on + strand at 896.665 kb on + strand at 896.675 kb on + strand at 896.675 kb on + strand at 896.676 kb on - strand at 896.703 kb on - strand at 896.706 kb on - strand, within CA265_RS03785 at 896.742 kb on + strand, within CA265_RS03785 at 896.742 kb on + strand, within CA265_RS03785 at 896.995 kb on + strand, within CA265_RS03785 at 896.996 kb on - strand, within CA265_RS03785 at 896.996 kb on - strand, within CA265_RS03785 at 896.996 kb on - strand, within CA265_RS03785 at 896.997 kb on + strand, within CA265_RS03785 at 896.997 kb on + strand, within CA265_RS03785 at 897.002 kb on + strand, within CA265_RS03785 at 897.166 kb on - strand at 897.213 kb on + strand at 897.214 kb on - strand at 897.236 kb on + strand at 897.236 kb on + strand at 897.243 kb on - strand at 897.325 kb on + strand, within CA265_RS03790 at 897.358 kb on + strand, within CA265_RS03790 at 897.389 kb on + strand, within CA265_RS03790 at 897.538 kb on + strand, within CA265_RS03790 at 897.604 kb on + strand, within CA265_RS03790 at 897.604 kb on + strand, within CA265_RS03790 at 897.604 kb on + strand, within CA265_RS03790 at 897.710 kb on + strand, within CA265_RS03790 at 897.781 kb on - strand at 897.786 kb on + strand at 897.786 kb on + strand at 897.856 kb on + strand, within CA265_RS03795 at 897.865 kb on + strand, within CA265_RS03795 at 897.912 kb on + strand, within CA265_RS03795 at 897.913 kb on - strand, within CA265_RS03795 at 897.913 kb on - strand, within CA265_RS03795 at 897.944 kb on - strand, within CA265_RS03795 at 897.958 kb on - strand, within CA265_RS03795 at 897.958 kb on - strand, within CA265_RS03795 at 897.959 kb on + strand, within CA265_RS03795 at 897.960 kb on - strand, within CA265_RS03795 at 897.960 kb on - strand, within CA265_RS03795 at 897.964 kb on - strand at 897.990 kb on + strand, within CA265_RS03795 at 898.034 kb on - strand, within CA265_RS03795 at 898.044 kb on - strand, within CA265_RS03795 at 898.047 kb on + strand, within CA265_RS03795 at 898.048 kb on - strand, within CA265_RS03795 at 898.096 kb on - strand, within CA265_RS03795 at 898.198 kb on + strand at 898.215 kb on + strand at 898.237 kb on + strand at 898.243 kb on - strand at 898.321 kb on + strand at 898.349 kb on + strand at 898.374 kb on - strand at 898.391 kb on + strand at 898.470 kb on - strand at 898.489 kb on + strand at 898.496 kb on - strand at 898.499 kb on + strand at 898.499 kb on + strand at 898.500 kb on - strand at 898.561 kb on - strand at 898.579 kb on - strand at 898.656 kb on - strand, within CA265_RS03800 at 898.668 kb on - strand, within CA265_RS03800
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 896,287 + CA265_RS03780 0.79 -0.2 896,396 + +0.3 896,397 - -0.0 896,404 + +0.3 896,404 + +0.6 896,405 - +1.3 896,405 - -0.7 896,430 + +0.1 896,430 + -0.1 896,430 + -0.8 896,450 + +0.1 896,511 - -0.1 896,635 - -0.3 896,665 + -0.1 896,665 + -0.8 896,675 + -1.1 896,675 + -1.1 896,676 - -0.7 896,703 - -0.2 896,706 - CA265_RS03785 0.10 -0.5 896,742 + CA265_RS03785 0.17 -1.2 896,742 + CA265_RS03785 0.17 -0.9 896,995 + CA265_RS03785 0.64 +0.4 896,996 - CA265_RS03785 0.64 +0.1 896,996 - CA265_RS03785 0.64 +1.1 896,996 - CA265_RS03785 0.64 +1.9 896,997 + CA265_RS03785 0.64 -0.3 896,997 + CA265_RS03785 0.64 -0.2 897,002 + CA265_RS03785 0.65 +0.3 897,166 - -0.3 897,213 + -0.1 897,214 - -0.0 897,236 + +0.2 897,236 + +0.8 897,243 - -0.6 897,325 + CA265_RS03790 0.11 -1.5 897,358 + CA265_RS03790 0.17 -2.2 897,389 + CA265_RS03790 0.23 -2.9 897,538 + CA265_RS03790 0.53 -1.3 897,604 + CA265_RS03790 0.67 +0.2 897,604 + CA265_RS03790 0.67 -0.7 897,604 + CA265_RS03790 0.67 -0.6 897,710 + CA265_RS03790 0.88 -0.5 897,781 - -0.7 897,786 + -0.0 897,786 + -1.2 897,856 + CA265_RS03795 0.23 +0.2 897,865 + CA265_RS03795 0.25 -0.1 897,912 + CA265_RS03795 0.37 +0.3 897,913 - CA265_RS03795 0.37 -0.2 897,913 - CA265_RS03795 0.37 -0.6 897,944 - CA265_RS03795 0.45 +0.2 897,958 - CA265_RS03795 0.49 -1.0 897,958 - CA265_RS03795 0.49 -0.0 897,959 + CA265_RS03795 0.49 -0.1 897,960 - CA265_RS03795 0.49 +0.3 897,960 - CA265_RS03795 0.49 +0.8 897,964 - -0.7 897,990 + CA265_RS03795 0.57 +0.2 898,034 - CA265_RS03795 0.68 -0.8 898,044 - CA265_RS03795 0.71 +0.6 898,047 + CA265_RS03795 0.72 -0.1 898,048 - CA265_RS03795 0.72 -1.2 898,096 - CA265_RS03795 0.84 +0.1 898,198 + -0.3 898,215 + +0.7 898,237 + +0.2 898,243 - +0.9 898,321 + +0.4 898,349 + +0.2 898,374 - -0.9 898,391 + -0.4 898,470 - -0.4 898,489 + +0.6 898,496 - -0.5 898,499 + +0.2 898,499 + -0.3 898,500 - -0.2 898,561 - +0.2 898,579 - -0.1 898,656 - CA265_RS03800 0.18 -0.2 898,668 - CA265_RS03800 0.20 -1.1
Or see this region's nucleotide sequence