Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03710 and CA265_RS03715 are separated by 90 nucleotides CA265_RS03715 and CA265_RS03720 are separated by 16 nucleotides CA265_RS03720 and CA265_RS03725 are separated by 170 nucleotides
CA265_RS03710: CA265_RS03710 - dihydroorotase, at 882,721 to 883,977
_RS03710
CA265_RS03715: CA265_RS03715 - hypothetical protein, at 884,068 to 884,736
_RS03715
CA265_RS03720: CA265_RS03720 - DUF4199 domain-containing protein, at 884,753 to 885,322
_RS03720
CA265_RS03725: CA265_RS03725 - glycosyltransferase, at 885,493 to 886,440
_RS03725
Position (kb) 884
885
886 Strain fitness (log2 ratio) -2
-1
0
1 at 883.991 kb on - strand at 884.028 kb on - strand at 884.028 kb on - strand at 884.036 kb on - strand at 884.067 kb on + strand at 884.067 kb on + strand at 884.113 kb on + strand at 884.196 kb on + strand, within CA265_RS03715 at 884.270 kb on + strand, within CA265_RS03715 at 884.324 kb on + strand, within CA265_RS03715 at 884.325 kb on - strand, within CA265_RS03715 at 884.413 kb on - strand, within CA265_RS03715 at 884.468 kb on + strand, within CA265_RS03715 at 884.582 kb on + strand, within CA265_RS03715 at 884.680 kb on - strand at 884.712 kb on - strand at 884.739 kb on + strand at 884.865 kb on - strand, within CA265_RS03720 at 884.907 kb on + strand, within CA265_RS03720 at 884.913 kb on + strand, within CA265_RS03720 at 884.916 kb on + strand, within CA265_RS03720 at 884.920 kb on - strand, within CA265_RS03720 at 884.949 kb on + strand, within CA265_RS03720 at 884.950 kb on - strand, within CA265_RS03720 at 885.015 kb on + strand, within CA265_RS03720 at 885.134 kb on - strand, within CA265_RS03720 at 885.156 kb on + strand, within CA265_RS03720 at 885.157 kb on - strand, within CA265_RS03720 at 885.238 kb on - strand, within CA265_RS03720 at 885.238 kb on - strand, within CA265_RS03720 at 885.239 kb on + strand, within CA265_RS03720 at 885.294 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 883,991 - -0.5 884,028 - -0.6 884,028 - -0.7 884,036 - +0.3 884,067 + +0.6 884,067 + -0.6 884,113 + +0.2 884,196 + CA265_RS03715 0.19 -0.6 884,270 + CA265_RS03715 0.30 +0.1 884,324 + CA265_RS03715 0.38 -1.9 884,325 - CA265_RS03715 0.38 -0.2 884,413 - CA265_RS03715 0.52 -1.8 884,468 + CA265_RS03715 0.60 -0.7 884,582 + CA265_RS03715 0.77 +1.3 884,680 - +0.2 884,712 - -0.3 884,739 + -0.0 884,865 - CA265_RS03720 0.20 -1.7 884,907 + CA265_RS03720 0.27 -2.1 884,913 + CA265_RS03720 0.28 -0.0 884,916 + CA265_RS03720 0.29 +0.3 884,920 - CA265_RS03720 0.29 -1.1 884,949 + CA265_RS03720 0.34 +1.0 884,950 - CA265_RS03720 0.35 -0.7 885,015 + CA265_RS03720 0.46 -0.4 885,134 - CA265_RS03720 0.67 -1.8 885,156 + CA265_RS03720 0.71 -0.7 885,157 - CA265_RS03720 0.71 -0.6 885,238 - CA265_RS03720 0.85 -1.6 885,238 - CA265_RS03720 0.85 +0.4 885,239 + CA265_RS03720 0.85 -1.1 885,294 + -0.5
Or see this region's nucleotide sequence