Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03585 and CA265_RS03590 are separated by 323 nucleotides CA265_RS03590 and CA265_RS03595 are separated by 110 nucleotides CA265_RS03595 and CA265_RS03600 are separated by 107 nucleotides
CA265_RS03585: CA265_RS03585 - ferredoxin, at 859,484 to 859,834
_RS03585
CA265_RS03590: CA265_RS03590 - universal stress protein, at 860,158 to 860,610
_RS03590
CA265_RS03595: CA265_RS03595 - hydrolase, at 860,721 to 861,506
_RS03595
CA265_RS03600: CA265_RS03600 - hypothetical protein, at 861,614 to 862,513
_RS03600
Position (kb)
860
861
862 Strain fitness (log2 ratio)
-2
-1
0
1 at 859.841 kb on - strand at 859.964 kb on - strand at 859.964 kb on - strand at 859.990 kb on + strand at 859.990 kb on + strand at 860.023 kb on + strand at 860.024 kb on - strand at 860.083 kb on + strand at 860.136 kb on + strand at 860.137 kb on - strand at 860.159 kb on + strand at 860.209 kb on - strand, within CA265_RS03590 at 860.209 kb on - strand, within CA265_RS03590 at 860.316 kb on + strand, within CA265_RS03590 at 860.344 kb on - strand, within CA265_RS03590 at 860.347 kb on + strand, within CA265_RS03590 at 860.348 kb on - strand, within CA265_RS03590 at 860.348 kb on - strand, within CA265_RS03590 at 860.351 kb on + strand, within CA265_RS03590 at 860.371 kb on - strand, within CA265_RS03590 at 860.403 kb on + strand, within CA265_RS03590 at 860.430 kb on - strand, within CA265_RS03590 at 860.470 kb on + strand, within CA265_RS03590 at 860.497 kb on - strand, within CA265_RS03590 at 860.510 kb on - strand, within CA265_RS03590 at 860.510 kb on - strand, within CA265_RS03590 at 860.572 kb on - strand at 860.575 kb on - strand at 860.575 kb on - strand at 860.575 kb on - strand at 860.575 kb on - strand at 860.619 kb on + strand at 860.682 kb on - strand at 860.682 kb on - strand at 860.718 kb on + strand at 860.718 kb on + strand at 860.719 kb on - strand at 860.756 kb on - strand at 860.756 kb on - strand at 860.824 kb on + strand, within CA265_RS03595 at 860.831 kb on + strand, within CA265_RS03595 at 861.080 kb on + strand, within CA265_RS03595 at 861.099 kb on + strand, within CA265_RS03595 at 861.169 kb on - strand, within CA265_RS03595 at 861.200 kb on - strand, within CA265_RS03595 at 861.245 kb on + strand, within CA265_RS03595 at 861.246 kb on - strand, within CA265_RS03595 at 861.371 kb on + strand, within CA265_RS03595 at 861.449 kb on + strand at 861.450 kb on - strand at 861.486 kb on - strand at 861.504 kb on + strand at 861.504 kb on + strand at 861.505 kb on - strand at 861.507 kb on + strand at 861.551 kb on - strand at 861.551 kb on - strand at 861.554 kb on - strand at 861.603 kb on + strand at 861.607 kb on - strand at 861.886 kb on - strand, within CA265_RS03600 at 861.908 kb on + strand, within CA265_RS03600 at 861.939 kb on + strand, within CA265_RS03600 at 861.940 kb on - strand, within CA265_RS03600 at 861.940 kb on - strand, within CA265_RS03600 at 861.970 kb on + strand, within CA265_RS03600 at 862.004 kb on + strand, within CA265_RS03600 at 862.048 kb on + strand, within CA265_RS03600 at 862.049 kb on - strand, within CA265_RS03600 at 862.049 kb on - strand, within CA265_RS03600 at 862.075 kb on + strand, within CA265_RS03600 at 862.107 kb on + strand, within CA265_RS03600 at 862.214 kb on + strand, within CA265_RS03600 at 862.214 kb on + strand, within CA265_RS03600 at 862.297 kb on + strand, within CA265_RS03600 at 862.321 kb on + strand, within CA265_RS03600 at 862.322 kb on - strand, within CA265_RS03600 at 862.334 kb on + strand at 862.335 kb on - strand, within CA265_RS03600 at 862.387 kb on + strand, within CA265_RS03600 at 862.388 kb on - strand, within CA265_RS03600 at 862.391 kb on + strand, within CA265_RS03600 at 862.392 kb on - strand, within CA265_RS03600 at 862.404 kb on - strand, within CA265_RS03600
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 859,841 - -0.2 859,964 - -0.4 859,964 - +0.7 859,990 + +0.1 859,990 + -0.0 860,023 + +0.5 860,024 - +0.1 860,083 + +0.2 860,136 + +0.1 860,137 - +0.8 860,159 + -0.1 860,209 - CA265_RS03590 0.11 -0.1 860,209 - CA265_RS03590 0.11 -0.2 860,316 + CA265_RS03590 0.35 -0.4 860,344 - CA265_RS03590 0.41 -0.2 860,347 + CA265_RS03590 0.42 -0.1 860,348 - CA265_RS03590 0.42 -0.0 860,348 - CA265_RS03590 0.42 -0.3 860,351 + CA265_RS03590 0.43 +0.4 860,371 - CA265_RS03590 0.47 -1.1 860,403 + CA265_RS03590 0.54 +0.1 860,430 - CA265_RS03590 0.60 -0.3 860,470 + CA265_RS03590 0.69 +0.2 860,497 - CA265_RS03590 0.75 -2.6 860,510 - CA265_RS03590 0.78 -0.5 860,510 - CA265_RS03590 0.78 -0.2 860,572 - +0.8 860,575 - -0.7 860,575 - -0.5 860,575 - +0.1 860,575 - -1.3 860,619 + +0.3 860,682 - +0.1 860,682 - -0.1 860,718 + +0.3 860,718 + +0.1 860,719 - +0.0 860,756 - -0.1 860,756 - -0.5 860,824 + CA265_RS03595 0.13 +0.3 860,831 + CA265_RS03595 0.14 +1.3 861,080 + CA265_RS03595 0.46 -0.5 861,099 + CA265_RS03595 0.48 -2.3 861,169 - CA265_RS03595 0.57 -1.2 861,200 - CA265_RS03595 0.61 -1.1 861,245 + CA265_RS03595 0.67 +0.3 861,246 - CA265_RS03595 0.67 -0.5 861,371 + CA265_RS03595 0.83 -0.5 861,449 + +0.3 861,450 - +0.3 861,486 - -0.7 861,504 + +1.1 861,504 + +1.3 861,505 - +0.4 861,507 + +0.0 861,551 - +0.4 861,551 - -0.7 861,554 - +0.3 861,603 + +0.9 861,607 - -1.2 861,886 - CA265_RS03600 0.30 +0.5 861,908 + CA265_RS03600 0.33 -0.9 861,939 + CA265_RS03600 0.36 +0.7 861,940 - CA265_RS03600 0.36 -0.4 861,940 - CA265_RS03600 0.36 -0.1 861,970 + CA265_RS03600 0.40 +0.5 862,004 + CA265_RS03600 0.43 -0.3 862,048 + CA265_RS03600 0.48 +0.7 862,049 - CA265_RS03600 0.48 +0.2 862,049 - CA265_RS03600 0.48 -0.7 862,075 + CA265_RS03600 0.51 +0.3 862,107 + CA265_RS03600 0.55 -1.6 862,214 + CA265_RS03600 0.67 -0.5 862,214 + CA265_RS03600 0.67 -0.8 862,297 + CA265_RS03600 0.76 -0.7 862,321 + CA265_RS03600 0.79 +0.2 862,322 - CA265_RS03600 0.79 -0.9 862,334 + +0.3 862,335 - CA265_RS03600 0.80 +0.2 862,387 + CA265_RS03600 0.86 -0.5 862,388 - CA265_RS03600 0.86 -0.2 862,391 + CA265_RS03600 0.86 -0.1 862,392 - CA265_RS03600 0.86 -0.1 862,404 - CA265_RS03600 0.88 +0.0
Or see this region's nucleotide sequence