Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02955 and CA265_RS02960 are separated by 121 nucleotides CA265_RS02960 and CA265_RS02965 are separated by 72 nucleotides CA265_RS02965 and CA265_RS02970 are separated by 99 nucleotides
CA265_RS02955: CA265_RS02955 - G-D-S-L family lipolytic protein, at 737,713 to 739,014
_RS02955
CA265_RS02960: CA265_RS02960 - tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA, at 739,136 to 740,050
_RS02960
CA265_RS02965: CA265_RS02965 - hypothetical protein, at 740,123 to 740,770
_RS02965
CA265_RS02970: CA265_RS02970 - tRNA nucleotidyltransferase, at 740,870 to 742,324
_RS02970
Position (kb)
739
740
741 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 738.219 kb on + strand, within CA265_RS02955 at 738.241 kb on - strand, within CA265_RS02955 at 738.285 kb on + strand, within CA265_RS02955 at 738.286 kb on - strand, within CA265_RS02955 at 738.286 kb on - strand, within CA265_RS02955 at 738.293 kb on + strand, within CA265_RS02955 at 738.309 kb on - strand, within CA265_RS02955 at 738.327 kb on - strand, within CA265_RS02955 at 738.331 kb on - strand, within CA265_RS02955 at 738.364 kb on - strand, within CA265_RS02955 at 738.377 kb on + strand, within CA265_RS02955 at 738.378 kb on - strand, within CA265_RS02955 at 738.407 kb on + strand, within CA265_RS02955 at 738.408 kb on - strand, within CA265_RS02955 at 738.426 kb on - strand, within CA265_RS02955 at 738.428 kb on + strand, within CA265_RS02955 at 738.428 kb on + strand, within CA265_RS02955 at 738.428 kb on + strand, within CA265_RS02955 at 738.429 kb on - strand, within CA265_RS02955 at 738.465 kb on + strand, within CA265_RS02955 at 738.491 kb on + strand, within CA265_RS02955 at 738.491 kb on + strand at 738.532 kb on + strand, within CA265_RS02955 at 738.532 kb on + strand, within CA265_RS02955 at 738.557 kb on + strand, within CA265_RS02955 at 738.650 kb on + strand, within CA265_RS02955 at 738.731 kb on - strand, within CA265_RS02955 at 738.739 kb on - strand, within CA265_RS02955 at 738.783 kb on + strand, within CA265_RS02955 at 738.788 kb on - strand, within CA265_RS02955 at 738.789 kb on + strand, within CA265_RS02955 at 738.790 kb on - strand, within CA265_RS02955 at 738.838 kb on - strand, within CA265_RS02955 at 738.840 kb on + strand, within CA265_RS02955 at 738.876 kb on - strand, within CA265_RS02955 at 738.928 kb on - strand at 738.928 kb on - strand at 738.946 kb on - strand at 738.949 kb on + strand at 738.958 kb on - strand at 738.958 kb on - strand at 738.976 kb on - strand at 738.986 kb on - strand at 738.995 kb on + strand at 739.012 kb on + strand at 739.013 kb on - strand at 739.032 kb on + strand at 739.046 kb on - strand at 739.124 kb on + strand at 739.125 kb on - strand at 739.328 kb on - strand, within CA265_RS02960 at 739.353 kb on + strand, within CA265_RS02960 at 739.388 kb on + strand, within CA265_RS02960 at 739.437 kb on + strand, within CA265_RS02960 at 739.440 kb on - strand, within CA265_RS02960 at 739.480 kb on + strand, within CA265_RS02960 at 739.545 kb on - strand, within CA265_RS02960 at 739.599 kb on + strand, within CA265_RS02960 at 739.641 kb on - strand, within CA265_RS02960 at 739.730 kb on + strand, within CA265_RS02960 at 739.734 kb on + strand, within CA265_RS02960 at 739.756 kb on + strand, within CA265_RS02960 at 739.881 kb on - strand, within CA265_RS02960 at 740.110 kb on - strand at 740.120 kb on + strand at 740.120 kb on + strand at 740.430 kb on - strand, within CA265_RS02965 at 740.479 kb on + strand, within CA265_RS02965 at 740.480 kb on - strand, within CA265_RS02965 at 740.488 kb on - strand, within CA265_RS02965 at 740.488 kb on - strand, within CA265_RS02965 at 740.515 kb on + strand, within CA265_RS02965 at 740.516 kb on - strand, within CA265_RS02965 at 740.516 kb on - strand, within CA265_RS02965 at 740.608 kb on + strand, within CA265_RS02965 at 740.619 kb on - strand, within CA265_RS02965 at 740.619 kb on - strand, within CA265_RS02965 at 740.648 kb on - strand, within CA265_RS02965 at 740.648 kb on - strand, within CA265_RS02965 at 740.669 kb on - strand, within CA265_RS02965 at 740.753 kb on - strand at 740.807 kb on - strand at 740.833 kb on + strand at 740.866 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 738,219 + CA265_RS02955 0.39 +0.3 738,241 - CA265_RS02955 0.41 -0.0 738,285 + CA265_RS02955 0.44 +0.8 738,286 - CA265_RS02955 0.44 +0.7 738,286 - CA265_RS02955 0.44 -0.4 738,293 + CA265_RS02955 0.45 -0.5 738,309 - CA265_RS02955 0.46 -0.6 738,327 - CA265_RS02955 0.47 +0.6 738,331 - CA265_RS02955 0.47 +0.0 738,364 - CA265_RS02955 0.50 +0.1 738,377 + CA265_RS02955 0.51 -0.4 738,378 - CA265_RS02955 0.51 +0.3 738,407 + CA265_RS02955 0.53 -0.1 738,408 - CA265_RS02955 0.53 -0.5 738,426 - CA265_RS02955 0.55 -0.3 738,428 + CA265_RS02955 0.55 +0.4 738,428 + CA265_RS02955 0.55 +0.8 738,428 + CA265_RS02955 0.55 -0.6 738,429 - CA265_RS02955 0.55 -0.4 738,465 + CA265_RS02955 0.58 +0.5 738,491 + CA265_RS02955 0.60 +0.6 738,491 + +0.3 738,532 + CA265_RS02955 0.63 +0.2 738,532 + CA265_RS02955 0.63 -1.3 738,557 + CA265_RS02955 0.65 +0.3 738,650 + CA265_RS02955 0.72 -0.3 738,731 - CA265_RS02955 0.78 -0.2 738,739 - CA265_RS02955 0.79 +0.0 738,783 + CA265_RS02955 0.82 -0.2 738,788 - CA265_RS02955 0.83 -0.3 738,789 + CA265_RS02955 0.83 -0.3 738,790 - CA265_RS02955 0.83 +0.8 738,838 - CA265_RS02955 0.86 +0.1 738,840 + CA265_RS02955 0.87 +1.1 738,876 - CA265_RS02955 0.89 -0.3 738,928 - +0.2 738,928 - -0.5 738,946 - -0.3 738,949 + -0.6 738,958 - -0.3 738,958 - -0.3 738,976 - +0.9 738,986 - -0.2 738,995 + +0.4 739,012 + -0.0 739,013 - +0.3 739,032 + +0.9 739,046 - +0.9 739,124 + +0.3 739,125 - +0.1 739,328 - CA265_RS02960 0.21 -1.7 739,353 + CA265_RS02960 0.24 +1.3 739,388 + CA265_RS02960 0.28 -4.0 739,437 + CA265_RS02960 0.33 +0.1 739,440 - CA265_RS02960 0.33 -3.0 739,480 + CA265_RS02960 0.38 -1.4 739,545 - CA265_RS02960 0.45 -2.5 739,599 + CA265_RS02960 0.51 -3.0 739,641 - CA265_RS02960 0.55 -0.7 739,730 + CA265_RS02960 0.65 -2.5 739,734 + CA265_RS02960 0.65 -0.7 739,756 + CA265_RS02960 0.68 -0.3 739,881 - CA265_RS02960 0.81 -0.3 740,110 - -0.3 740,120 + +0.2 740,120 + +0.0 740,430 - CA265_RS02965 0.47 +0.7 740,479 + CA265_RS02965 0.55 +0.8 740,480 - CA265_RS02965 0.55 +0.9 740,488 - CA265_RS02965 0.56 +0.6 740,488 - CA265_RS02965 0.56 -0.3 740,515 + CA265_RS02965 0.60 +1.0 740,516 - CA265_RS02965 0.61 -0.6 740,516 - CA265_RS02965 0.61 +0.0 740,608 + CA265_RS02965 0.75 +0.6 740,619 - CA265_RS02965 0.77 +0.0 740,619 - CA265_RS02965 0.77 +0.8 740,648 - CA265_RS02965 0.81 -0.9 740,648 - CA265_RS02965 0.81 +0.8 740,669 - CA265_RS02965 0.84 +0.7 740,753 - -0.1 740,807 - +0.3 740,833 + -0.7 740,866 + +1.3
Or see this region's nucleotide sequence