Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02100 and CA265_RS02105 are separated by 12 nucleotides CA265_RS02105 and CA265_RS02110 overlap by 4 nucleotides CA265_RS02110 and CA265_RS02115 are separated by 20 nucleotides CA265_RS02115 and CA265_RS02120 are separated by 3 nucleotides
CA265_RS02100: CA265_RS02100 - hypothetical protein, at 529,862 to 530,299
_RS02100
CA265_RS02105: CA265_RS02105 - hypothetical protein, at 530,312 to 530,746
_RS02105
CA265_RS02110: CA265_RS02110 - hypothetical protein, at 530,743 to 531,192
_RS02110
CA265_RS02115: CA265_RS02115 - Fur family transcriptional regulator, at 531,213 to 531,650
_RS02115
CA265_RS02120: CA265_RS02120 - acyl transferase, at 531,654 to 532,652
_RS02120
Position (kb) 531
532 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 530.280 kb on + strand at 530.370 kb on + strand, within CA265_RS02105 at 530.419 kb on - strand, within CA265_RS02105 at 530.488 kb on + strand, within CA265_RS02105 at 530.488 kb on + strand, within CA265_RS02105 at 530.497 kb on - strand, within CA265_RS02105 at 530.497 kb on - strand, within CA265_RS02105 at 530.516 kb on - strand, within CA265_RS02105 at 530.519 kb on - strand, within CA265_RS02105 at 530.527 kb on + strand, within CA265_RS02105 at 530.539 kb on + strand, within CA265_RS02105 at 530.540 kb on - strand, within CA265_RS02105 at 530.588 kb on + strand, within CA265_RS02105 at 530.625 kb on + strand, within CA265_RS02105 at 530.625 kb on + strand, within CA265_RS02105 at 530.626 kb on - strand, within CA265_RS02105 at 530.754 kb on - strand at 530.756 kb on - strand at 530.756 kb on - strand at 530.784 kb on + strand at 530.892 kb on + strand, within CA265_RS02110 at 530.892 kb on + strand, within CA265_RS02110 at 531.026 kb on - strand, within CA265_RS02110 at 531.089 kb on - strand, within CA265_RS02110 at 531.091 kb on - strand, within CA265_RS02110 at 531.191 kb on - strand at 531.198 kb on + strand at 531.199 kb on - strand at 531.207 kb on - strand at 531.224 kb on + strand at 531.334 kb on - strand, within CA265_RS02115 at 531.379 kb on - strand, within CA265_RS02115 at 531.379 kb on - strand, within CA265_RS02115 at 531.428 kb on - strand, within CA265_RS02115 at 531.450 kb on + strand, within CA265_RS02115 at 531.450 kb on + strand, within CA265_RS02115 at 531.451 kb on - strand, within CA265_RS02115 at 531.502 kb on + strand, within CA265_RS02115 at 531.502 kb on + strand, within CA265_RS02115 at 531.503 kb on - strand, within CA265_RS02115 at 531.510 kb on - strand, within CA265_RS02115 at 531.585 kb on - strand, within CA265_RS02115 at 531.643 kb on + strand at 531.643 kb on + strand at 531.643 kb on + strand at 531.655 kb on + strand at 531.671 kb on + strand at 531.733 kb on - strand at 531.798 kb on - strand, within CA265_RS02120 at 531.904 kb on + strand, within CA265_RS02120 at 531.919 kb on - strand, within CA265_RS02120 at 531.934 kb on + strand, within CA265_RS02120 at 531.942 kb on - strand, within CA265_RS02120 at 532.076 kb on + strand, within CA265_RS02120 at 532.076 kb on + strand, within CA265_RS02120 at 532.094 kb on - strand, within CA265_RS02120 at 532.169 kb on + strand, within CA265_RS02120 at 532.174 kb on - strand, within CA265_RS02120 at 532.227 kb on - strand, within CA265_RS02120 at 532.228 kb on + strand, within CA265_RS02120 at 532.229 kb on - strand, within CA265_RS02120 at 532.327 kb on + strand, within CA265_RS02120 at 532.327 kb on + strand, within CA265_RS02120 at 532.357 kb on - strand, within CA265_RS02120 at 532.357 kb on - strand, within CA265_RS02120 at 532.385 kb on + strand, within CA265_RS02120 at 532.424 kb on + strand, within CA265_RS02120 at 532.513 kb on - strand, within CA265_RS02120 at 532.526 kb on - strand, within CA265_RS02120 at 532.558 kb on - strand at 532.615 kb on - strand at 532.622 kb on + strand at 532.622 kb on + strand at 532.623 kb on - strand at 532.623 kb on - strand at 532.649 kb on - strand at 532.649 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 530,280 + +0.0 530,370 + CA265_RS02105 0.13 -0.6 530,419 - CA265_RS02105 0.25 -0.0 530,488 + CA265_RS02105 0.40 -0.6 530,488 + CA265_RS02105 0.40 -0.8 530,497 - CA265_RS02105 0.43 +0.4 530,497 - CA265_RS02105 0.43 +0.0 530,516 - CA265_RS02105 0.47 -0.7 530,519 - CA265_RS02105 0.48 -0.3 530,527 + CA265_RS02105 0.49 -0.6 530,539 + CA265_RS02105 0.52 -0.6 530,540 - CA265_RS02105 0.52 +0.3 530,588 + CA265_RS02105 0.63 +0.1 530,625 + CA265_RS02105 0.72 +0.3 530,625 + CA265_RS02105 0.72 +0.3 530,626 - CA265_RS02105 0.72 +0.2 530,754 - +1.0 530,756 - +0.2 530,756 - -0.2 530,784 + -0.7 530,892 + CA265_RS02110 0.33 +1.4 530,892 + CA265_RS02110 0.33 +1.0 531,026 - CA265_RS02110 0.63 +0.1 531,089 - CA265_RS02110 0.77 -0.1 531,091 - CA265_RS02110 0.77 -1.0 531,191 - -0.2 531,198 + -0.7 531,199 - -0.6 531,207 - -2.5 531,224 + -0.8 531,334 - CA265_RS02115 0.28 -0.7 531,379 - CA265_RS02115 0.38 -0.7 531,379 - CA265_RS02115 0.38 -1.2 531,428 - CA265_RS02115 0.49 -1.2 531,450 + CA265_RS02115 0.54 -1.0 531,450 + CA265_RS02115 0.54 -0.2 531,451 - CA265_RS02115 0.54 -3.0 531,502 + CA265_RS02115 0.66 -0.3 531,502 + CA265_RS02115 0.66 -0.8 531,503 - CA265_RS02115 0.66 +0.1 531,510 - CA265_RS02115 0.68 -1.0 531,585 - CA265_RS02115 0.85 +0.3 531,643 + -0.3 531,643 + -0.2 531,643 + -2.5 531,655 + +0.7 531,671 + -0.4 531,733 - -0.1 531,798 - CA265_RS02120 0.14 -1.3 531,904 + CA265_RS02120 0.25 -1.2 531,919 - CA265_RS02120 0.27 -1.0 531,934 + CA265_RS02120 0.28 -1.1 531,942 - CA265_RS02120 0.29 -1.3 532,076 + CA265_RS02120 0.42 -0.6 532,076 + CA265_RS02120 0.42 -0.2 532,094 - CA265_RS02120 0.44 -1.2 532,169 + CA265_RS02120 0.52 -0.1 532,174 - CA265_RS02120 0.52 -0.9 532,227 - CA265_RS02120 0.57 -0.4 532,228 + CA265_RS02120 0.57 -1.3 532,229 - CA265_RS02120 0.58 -1.8 532,327 + CA265_RS02120 0.67 +1.0 532,327 + CA265_RS02120 0.67 -0.6 532,357 - CA265_RS02120 0.70 +0.2 532,357 - CA265_RS02120 0.70 -1.8 532,385 + CA265_RS02120 0.73 -1.2 532,424 + CA265_RS02120 0.77 -0.2 532,513 - CA265_RS02120 0.86 -0.7 532,526 - CA265_RS02120 0.87 -2.6 532,558 - -2.2 532,615 - -1.1 532,622 + -0.3 532,622 + -1.1 532,623 - -0.5 532,623 - -1.3 532,649 - -0.3 532,649 - -0.4
Or see this region's nucleotide sequence