Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02035 and CA265_RS02040 are separated by 185 nucleotides CA265_RS02040 and CA265_RS02045 are separated by 122 nucleotides CA265_RS02045 and CA265_RS02050 are separated by 220 nucleotides
CA265_RS02035: CA265_RS02035 - hypothetical protein, at 515,966 to 516,949
_RS02035
CA265_RS02040: CA265_RS02040 - N-acetyltransferase, at 517,135 to 517,617
_RS02040
CA265_RS02045: CA265_RS02045 - hypothetical protein, at 517,740 to 518,030
_RS02045
CA265_RS02050: CA265_RS02050 - 4-hydroxy-tetrahydrodipicolinate synthase, at 518,251 to 519,138
_RS02050
Position (kb) 517
518
519 Strain fitness (log2 ratio) -2
-1
0
1 at 516.757 kb on + strand, within CA265_RS02035 at 516.812 kb on - strand, within CA265_RS02035 at 516.843 kb on - strand, within CA265_RS02035 at 516.875 kb on + strand at 516.876 kb on - strand at 517.013 kb on + strand at 517.015 kb on + strand at 517.016 kb on - strand at 517.016 kb on - strand at 517.069 kb on - strand at 517.069 kb on - strand at 517.094 kb on + strand at 517.096 kb on + strand at 517.179 kb on + strand at 517.184 kb on - strand, within CA265_RS02040 at 517.264 kb on - strand, within CA265_RS02040 at 517.340 kb on - strand, within CA265_RS02040 at 517.365 kb on - strand, within CA265_RS02040 at 517.373 kb on - strand, within CA265_RS02040 at 517.404 kb on - strand, within CA265_RS02040 at 517.532 kb on + strand, within CA265_RS02040 at 517.533 kb on - strand, within CA265_RS02040 at 517.568 kb on + strand, within CA265_RS02040 at 517.616 kb on - strand at 517.638 kb on + strand at 517.642 kb on - strand at 517.643 kb on + strand at 517.643 kb on + strand at 517.757 kb on - strand at 517.821 kb on - strand, within CA265_RS02045 at 517.868 kb on + strand, within CA265_RS02045 at 518.016 kb on + strand at 518.017 kb on - strand at 518.064 kb on - strand at 518.079 kb on + strand at 518.082 kb on + strand at 518.082 kb on + strand at 518.083 kb on - strand at 518.083 kb on - strand at 518.088 kb on + strand at 518.147 kb on - strand at 518.234 kb on + strand at 518.253 kb on - strand at 518.257 kb on + strand at 518.257 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 516,757 + CA265_RS02035 0.80 +0.6 516,812 - CA265_RS02035 0.86 +0.2 516,843 - CA265_RS02035 0.89 -0.2 516,875 + -1.6 516,876 - -0.6 517,013 + -0.3 517,015 + -0.3 517,016 - -0.2 517,016 - -0.1 517,069 - +0.1 517,069 - +0.3 517,094 + -0.1 517,096 + -0.2 517,179 + -0.4 517,184 - CA265_RS02040 0.10 -0.1 517,264 - CA265_RS02040 0.27 +0.3 517,340 - CA265_RS02040 0.42 +0.6 517,365 - CA265_RS02040 0.48 -0.3 517,373 - CA265_RS02040 0.49 +1.1 517,404 - CA265_RS02040 0.56 +0.3 517,532 + CA265_RS02040 0.82 -0.2 517,533 - CA265_RS02040 0.82 -0.2 517,568 + CA265_RS02040 0.90 -0.6 517,616 - -0.1 517,638 + +0.5 517,642 - -0.8 517,643 + +0.1 517,643 + -0.4 517,757 - -0.8 517,821 - CA265_RS02045 0.28 -1.4 517,868 + CA265_RS02045 0.44 -1.2 518,016 + -0.0 518,017 - +0.2 518,064 - +0.2 518,079 + +0.1 518,082 + +0.2 518,082 + -0.2 518,083 - -1.2 518,083 - -1.9 518,088 + +0.3 518,147 - -0.8 518,234 + -0.0 518,253 - -0.4 518,257 + -0.1 518,257 + -0.4
Or see this region's nucleotide sequence