Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS01375 and CA265_RS01380 are separated by 3 nucleotides CA265_RS01380 and CA265_RS01385 are separated by 187 nucleotides CA265_RS01385 and CA265_RS01390 overlap by 17 nucleotides
CA265_RS01375: CA265_RS01375 - cytochrome C oxidase Cbb3, at 346,111 to 348,252
_RS01375
CA265_RS01380: CA265_RS01380 - hypothetical protein, at 348,256 to 348,438
_RS01380
CA265_RS01385: CA265_RS01385 - cytochrome C, at 348,626 to 349,393
_RS01385
CA265_RS01390: CA265_RS01390 - cytochrome c oxidase accessory protein CcoG, at 349,377 to 350,756
_RS01390
Position (kb) 348
349 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 347.273 kb on + strand, within CA265_RS01375 at 347.273 kb on + strand, within CA265_RS01375 at 347.398 kb on - strand, within CA265_RS01375 at 347.399 kb on + strand, within CA265_RS01375 at 347.438 kb on - strand, within CA265_RS01375 at 347.438 kb on - strand, within CA265_RS01375 at 347.451 kb on - strand, within CA265_RS01375 at 347.464 kb on + strand, within CA265_RS01375 at 347.464 kb on + strand, within CA265_RS01375 at 347.626 kb on - strand, within CA265_RS01375 at 347.744 kb on - strand, within CA265_RS01375 at 347.744 kb on - strand, within CA265_RS01375 at 347.762 kb on + strand, within CA265_RS01375 at 347.823 kb on + strand, within CA265_RS01375 at 347.872 kb on - strand, within CA265_RS01375 at 347.912 kb on - strand, within CA265_RS01375 at 347.929 kb on + strand, within CA265_RS01375 at 347.930 kb on - strand, within CA265_RS01375 at 347.973 kb on + strand, within CA265_RS01375 at 348.082 kb on - strand at 348.101 kb on + strand at 348.134 kb on + strand at 348.173 kb on + strand at 348.222 kb on + strand at 348.279 kb on - strand, within CA265_RS01380 at 348.295 kb on + strand, within CA265_RS01380 at 348.296 kb on - strand, within CA265_RS01380 at 348.362 kb on + strand, within CA265_RS01380 at 348.399 kb on + strand, within CA265_RS01380 at 348.448 kb on - strand at 348.462 kb on + strand at 348.556 kb on + strand at 348.557 kb on - strand at 348.620 kb on - strand at 348.642 kb on + strand at 348.643 kb on - strand at 348.656 kb on + strand at 348.684 kb on + strand at 348.794 kb on + strand, within CA265_RS01385 at 348.795 kb on - strand, within CA265_RS01385 at 348.905 kb on + strand, within CA265_RS01385 at 348.971 kb on + strand, within CA265_RS01385 at 348.991 kb on + strand, within CA265_RS01385 at 349.024 kb on + strand, within CA265_RS01385 at 349.025 kb on - strand, within CA265_RS01385 at 349.055 kb on - strand, within CA265_RS01385 at 349.131 kb on - strand, within CA265_RS01385 at 349.162 kb on - strand, within CA265_RS01385 at 349.256 kb on - strand, within CA265_RS01385 at 349.261 kb on - strand, within CA265_RS01385 at 349.330 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 347,273 + CA265_RS01375 0.54 +0.8 347,273 + CA265_RS01375 0.54 -1.0 347,398 - CA265_RS01375 0.60 +0.3 347,399 + CA265_RS01375 0.60 +0.7 347,438 - CA265_RS01375 0.62 -0.2 347,438 - CA265_RS01375 0.62 -0.6 347,451 - CA265_RS01375 0.63 -0.2 347,464 + CA265_RS01375 0.63 -1.7 347,464 + CA265_RS01375 0.63 -0.6 347,626 - CA265_RS01375 0.71 -0.2 347,744 - CA265_RS01375 0.76 +0.6 347,744 - CA265_RS01375 0.76 +0.2 347,762 + CA265_RS01375 0.77 +0.6 347,823 + CA265_RS01375 0.80 -0.2 347,872 - CA265_RS01375 0.82 -3.1 347,912 - CA265_RS01375 0.84 +0.2 347,929 + CA265_RS01375 0.85 +0.4 347,930 - CA265_RS01375 0.85 +0.2 347,973 + CA265_RS01375 0.87 -1.1 348,082 - +0.2 348,101 + -2.9 348,134 + -0.3 348,173 + +0.4 348,222 + -3.4 348,279 - CA265_RS01380 0.13 -1.1 348,295 + CA265_RS01380 0.21 -0.6 348,296 - CA265_RS01380 0.22 -0.5 348,362 + CA265_RS01380 0.58 -0.5 348,399 + CA265_RS01380 0.78 -1.3 348,448 - +0.4 348,462 + -0.2 348,556 + -1.1 348,557 - -0.0 348,620 - +0.4 348,642 + -0.2 348,643 - -0.5 348,656 + -2.0 348,684 + -0.9 348,794 + CA265_RS01385 0.22 +0.2 348,795 - CA265_RS01385 0.22 -0.5 348,905 + CA265_RS01385 0.36 -0.8 348,971 + CA265_RS01385 0.45 -0.3 348,991 + CA265_RS01385 0.48 +0.7 349,024 + CA265_RS01385 0.52 -0.1 349,025 - CA265_RS01385 0.52 -0.6 349,055 - CA265_RS01385 0.56 +0.2 349,131 - CA265_RS01385 0.66 +1.4 349,162 - CA265_RS01385 0.70 -0.6 349,256 - CA265_RS01385 0.82 -0.5 349,261 - CA265_RS01385 0.83 +0.2 349,330 - -0.3
Or see this region's nucleotide sequence