Experiment: R2A with Neomycin 0.004 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS00020 and CA265_RS00025 are separated by 3 nucleotides CA265_RS00025 and CA265_RS00030 are separated by 82 nucleotides CA265_RS00030 and CA265_RS00035 are separated by 483 nucleotides
CA265_RS00020: CA265_RS00020 - methanol dehydrogenase, at 2,628 to 3,401
_RS00020
CA265_RS00025: CA265_RS00025 - hypothetical protein, at 3,405 to 3,848
_RS00025
CA265_RS00030: CA265_RS00030 - LemA family protein, at 3,931 to 4,512
_RS00030
CA265_RS00035: CA265_RS00035 - FAD-binding protein, at 4,996 to 6,537
_RS00035
Position (kb) 3
4
5 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3.008 kb on + strand, within CA265_RS00020 at 3.009 kb on - strand, within CA265_RS00020 at 3.041 kb on + strand, within CA265_RS00020 at 3.042 kb on - strand, within CA265_RS00020 at 3.117 kb on - strand, within CA265_RS00020 at 3.175 kb on + strand, within CA265_RS00020 at 3.185 kb on + strand, within CA265_RS00020 at 3.185 kb on + strand, within CA265_RS00020 at 3.260 kb on + strand, within CA265_RS00020 at 3.260 kb on + strand, within CA265_RS00020 at 3.291 kb on + strand, within CA265_RS00020 at 3.294 kb on + strand, within CA265_RS00020 at 3.294 kb on + strand, within CA265_RS00020 at 3.299 kb on + strand, within CA265_RS00020 at 3.364 kb on - strand at 3.406 kb on + strand at 3.407 kb on - strand at 3.417 kb on + strand at 3.418 kb on - strand at 3.418 kb on - strand at 3.572 kb on + strand, within CA265_RS00025 at 3.572 kb on + strand, within CA265_RS00025 at 3.597 kb on + strand, within CA265_RS00025 at 3.758 kb on + strand, within CA265_RS00025 at 3.759 kb on - strand, within CA265_RS00025 at 3.767 kb on + strand, within CA265_RS00025 at 3.848 kb on + strand at 3.848 kb on + strand at 3.848 kb on + strand at 3.849 kb on - strand at 3.854 kb on + strand at 3.854 kb on + strand at 3.857 kb on + strand at 3.858 kb on - strand at 3.858 kb on - strand at 3.861 kb on + strand at 3.880 kb on + strand at 3.881 kb on - strand at 3.891 kb on + strand at 3.892 kb on - strand at 3.912 kb on - strand at 3.913 kb on + strand at 3.914 kb on - strand at 3.914 kb on - strand at 3.914 kb on - strand at 3.914 kb on - strand at 3.987 kb on + strand at 3.988 kb on - strand at 4.111 kb on + strand, within CA265_RS00030 at 4.150 kb on + strand, within CA265_RS00030 at 4.180 kb on + strand, within CA265_RS00030 at 4.306 kb on + strand, within CA265_RS00030 at 4.340 kb on - strand, within CA265_RS00030 at 4.440 kb on + strand, within CA265_RS00030 at 4.441 kb on - strand, within CA265_RS00030 at 4.476 kb on + strand at 4.477 kb on - strand at 4.477 kb on - strand at 4.502 kb on - strand at 4.547 kb on + strand at 4.566 kb on - strand at 4.769 kb on + strand at 4.770 kb on - strand at 4.772 kb on + strand at 4.834 kb on - strand at 4.926 kb on - strand at 4.944 kb on - strand at 4.959 kb on + strand at 4.959 kb on + strand at 4.968 kb on - strand at 4.979 kb on + strand at 5.204 kb on + strand, within CA265_RS00035 at 5.206 kb on + strand, within CA265_RS00035 at 5.214 kb on + strand, within CA265_RS00035 at 5.214 kb on + strand, within CA265_RS00035 at 5.247 kb on - strand, within CA265_RS00035 at 5.253 kb on - strand, within CA265_RS00035 at 5.300 kb on - strand, within CA265_RS00035 at 5.342 kb on + strand, within CA265_RS00035 at 5.342 kb on + strand, within CA265_RS00035 at 5.408 kb on - strand, within CA265_RS00035 at 5.484 kb on + strand, within CA265_RS00035
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Neomycin 0.004 mg/ml remove 3,008 + CA265_RS00020 0.49 +0.0 3,009 - CA265_RS00020 0.49 -0.1 3,041 + CA265_RS00020 0.53 -0.5 3,042 - CA265_RS00020 0.53 -0.9 3,117 - CA265_RS00020 0.63 -0.8 3,175 + CA265_RS00020 0.71 +0.4 3,185 + CA265_RS00020 0.72 -1.6 3,185 + CA265_RS00020 0.72 -1.0 3,260 + CA265_RS00020 0.82 +0.6 3,260 + CA265_RS00020 0.82 -0.7 3,291 + CA265_RS00020 0.86 -0.9 3,294 + CA265_RS00020 0.86 -1.1 3,294 + CA265_RS00020 0.86 -0.5 3,299 + CA265_RS00020 0.87 -0.6 3,364 - -0.7 3,406 + -0.1 3,407 - -0.2 3,417 + -0.2 3,418 - +0.7 3,418 - +0.5 3,572 + CA265_RS00025 0.38 +0.5 3,572 + CA265_RS00025 0.38 +0.1 3,597 + CA265_RS00025 0.43 +0.2 3,758 + CA265_RS00025 0.80 +0.1 3,759 - CA265_RS00025 0.80 -1.2 3,767 + CA265_RS00025 0.82 +0.1 3,848 + -0.1 3,848 + -2.7 3,848 + -0.0 3,849 - -1.7 3,854 + -0.1 3,854 + -0.9 3,857 + -0.2 3,858 - +0.2 3,858 - -1.6 3,861 + +0.1 3,880 + +0.5 3,881 - -3.4 3,891 + -0.1 3,892 - -0.4 3,912 - -0.8 3,913 + -0.0 3,914 - -0.7 3,914 - +0.0 3,914 - +0.1 3,914 - +0.4 3,987 + -0.4 3,988 - -0.5 4,111 + CA265_RS00030 0.31 +0.4 4,150 + CA265_RS00030 0.38 -1.1 4,180 + CA265_RS00030 0.43 -0.5 4,306 + CA265_RS00030 0.64 +0.5 4,340 - CA265_RS00030 0.70 -1.9 4,440 + CA265_RS00030 0.87 -1.6 4,441 - CA265_RS00030 0.88 -1.5 4,476 + +0.6 4,477 - -0.4 4,477 - -0.1 4,502 - -1.0 4,547 + -0.7 4,566 - +0.3 4,769 + -0.0 4,770 - +0.4 4,772 + +1.3 4,834 - +0.0 4,926 - +1.5 4,944 - -0.0 4,959 + -0.6 4,959 + +0.6 4,968 - +0.6 4,979 + -0.9 5,204 + CA265_RS00035 0.13 +0.2 5,206 + CA265_RS00035 0.14 -0.3 5,214 + CA265_RS00035 0.14 -1.2 5,214 + CA265_RS00035 0.14 +0.4 5,247 - CA265_RS00035 0.16 -0.0 5,253 - CA265_RS00035 0.17 -1.0 5,300 - CA265_RS00035 0.20 +0.8 5,342 + CA265_RS00035 0.22 -0.4 5,342 + CA265_RS00035 0.22 +0.6 5,408 - CA265_RS00035 0.27 +0.1 5,484 + CA265_RS00035 0.32 +0.7
Or see this region's nucleotide sequence