Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gltD and Shewana3_3052 are separated by 247 nucleotides Shewana3_3052 and Shewana3_3053 are separated by 31 nucleotides Shewana3_3053 and Shewana3_3054 are separated by 55 nucleotides Shewana3_3054 and Shewana3_3055 overlap by 4 nucleotides
Shewana3_3051: gltD - glutamate synthase subunit beta (RefSeq), at 3,658,672 to 3,660,078
gltD
Shewana3_3052: Shewana3_3052 - methylthioadenosine nucleosidase (RefSeq), at 3,660,326 to 3,661,036
_3052
Shewana3_3053: Shewana3_3053 - cobalamin biosynthesis protein CbiB (RefSeq), at 3,661,068 to 3,662,057
_3053
Shewana3_3054: Shewana3_3054 - hypothetical protein (RefSeq), at 3,662,113 to 3,662,490
_3054
Shewana3_3055: Shewana3_3055 - hypothetical protein (RefSeq), at 3,662,487 to 3,663,113
_3055
Position (kb)
3661
3662
3663 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3660.159 kb on + strand at 3660.159 kb on + strand at 3660.160 kb on + strand at 3660.167 kb on - strand at 3660.167 kb on - strand at 3660.242 kb on + strand at 3661.206 kb on - strand, within Shewana3_3053 at 3661.393 kb on + strand, within Shewana3_3053 at 3661.406 kb on - strand, within Shewana3_3053 at 3661.611 kb on - strand, within Shewana3_3053 at 3661.878 kb on + strand, within Shewana3_3053 at 3661.908 kb on - strand, within Shewana3_3053 at 3661.918 kb on - strand, within Shewana3_3053 at 3662.042 kb on + strand at 3662.211 kb on - strand, within Shewana3_3054 at 3662.220 kb on + strand, within Shewana3_3054 at 3662.313 kb on - strand, within Shewana3_3054 at 3662.315 kb on + strand, within Shewana3_3054 at 3662.714 kb on - strand, within Shewana3_3055 at 3662.770 kb on - strand, within Shewana3_3055 at 3662.872 kb on - strand, within Shewana3_3055 at 3662.889 kb on + strand, within Shewana3_3055 at 3662.922 kb on + strand, within Shewana3_3055
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 3,660,159 + +3.3 3,660,159 + +3.3 3,660,160 + -0.6 3,660,167 - +1.0 3,660,167 - +1.8 3,660,242 + +0.5 3,661,206 - Shewana3_3053 0.14 +1.0 3,661,393 + Shewana3_3053 0.33 +0.5 3,661,406 - Shewana3_3053 0.34 +0.3 3,661,611 - Shewana3_3053 0.55 -2.0 3,661,878 + Shewana3_3053 0.82 -1.3 3,661,908 - Shewana3_3053 0.85 -0.3 3,661,918 - Shewana3_3053 0.86 -0.7 3,662,042 + -0.8 3,662,211 - Shewana3_3054 0.26 -0.5 3,662,220 + Shewana3_3054 0.28 +0.3 3,662,313 - Shewana3_3054 0.53 -1.0 3,662,315 + Shewana3_3054 0.53 -0.3 3,662,714 - Shewana3_3055 0.36 +0.2 3,662,770 - Shewana3_3055 0.45 -0.4 3,662,872 - Shewana3_3055 0.61 +1.2 3,662,889 + Shewana3_3055 0.64 -0.5 3,662,922 + Shewana3_3055 0.69 +0.1
Or see this region's nucleotide sequence