Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nhaR and Shewana3_3037 are separated by 95 nucleotides Shewana3_3037 and Shewana3_3038 are separated by 2 nucleotides Shewana3_3038 and thyA are separated by 118 nucleotides thyA and Shewana3_3040 overlap by 1 nucleotides
Shewana3_3036: nhaR - transcriptional activator NhaR (RefSeq), at 3,640,469 to 3,641,377
nhaR
Shewana3_3037: Shewana3_3037 - hypothetical protein (RefSeq), at 3,641,473 to 3,641,859
_3037
Shewana3_3038: Shewana3_3038 - Na+/H+ antiporter NhaA (RefSeq), at 3,641,862 to 3,643,031
_3038
Shewana3_3039: thyA - thymidylate synthase (RefSeq), at 3,643,150 to 3,643,944
thyA
Shewana3_3040: Shewana3_3040 - prolipoprotein diacylglyceryl transferase (RefSeq), at 3,643,944 to 3,644,750
_3040
Position (kb)
3641
3642
3643
3644 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7
8
9
10 at 3640.865 kb on + strand, within nhaR at 3641.137 kb on + strand, within nhaR at 3641.276 kb on + strand, within nhaR at 3641.290 kb on + strand at 3641.298 kb on - strand at 3641.305 kb on - strand at 3641.411 kb on + strand at 3641.421 kb on + strand at 3641.428 kb on + strand at 3641.647 kb on + strand, within Shewana3_3037 at 3641.665 kb on - strand, within Shewana3_3037 at 3641.665 kb on - strand, within Shewana3_3037 at 3641.929 kb on - strand at 3641.961 kb on + strand at 3642.180 kb on - strand, within Shewana3_3038 at 3642.264 kb on + strand, within Shewana3_3038 at 3642.369 kb on + strand, within Shewana3_3038 at 3642.375 kb on - strand, within Shewana3_3038 at 3642.438 kb on + strand, within Shewana3_3038 at 3642.446 kb on - strand, within Shewana3_3038 at 3642.446 kb on - strand, within Shewana3_3038 at 3642.775 kb on + strand, within Shewana3_3038 at 3642.836 kb on - strand, within Shewana3_3038 at 3642.853 kb on + strand, within Shewana3_3038
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 3,640,865 + nhaR Shewana3_3036 0.44 -0.3 3,641,137 + nhaR Shewana3_3036 0.73 -0.9 3,641,276 + nhaR Shewana3_3036 0.89 -0.9 3,641,290 + -0.3 3,641,298 - +1.2 3,641,305 - -0.6 3,641,411 + -0.5 3,641,421 + +0.1 3,641,428 + -0.6 3,641,647 + Shewana3_3037 0.45 +2.3 3,641,665 - Shewana3_3037 0.50 +5.2 3,641,665 - Shewana3_3037 0.50 +0.0 3,641,929 - -2.1 3,641,961 + +2.3 3,642,180 - Shewana3_3038 0.27 +0.8 3,642,264 + Shewana3_3038 0.34 +1.3 3,642,369 + Shewana3_3038 0.43 +0.0 3,642,375 - Shewana3_3038 0.44 -1.9 3,642,438 + Shewana3_3038 0.49 -2.5 3,642,446 - Shewana3_3038 0.50 +0.0 3,642,446 - Shewana3_3038 0.50 -0.2 3,642,775 + Shewana3_3038 0.78 +9.8 3,642,836 - Shewana3_3038 0.83 +1.3 3,642,853 + Shewana3_3038 0.85 +0.0
Or see this region's nucleotide sequence