Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2503 and Shewana3_2504 are separated by 362 nucleotides Shewana3_2504 and Shewana3_2505 are separated by 130 nucleotides Shewana3_2505 and Shewana3_2506 overlap by 14 nucleotides Shewana3_2506 and Shewana3_2507 overlap by 4 nucleotides
Shewana3_2503: Shewana3_2503 - purine nucleoside phosphorylase (RefSeq), at 2,994,813 to 2,995,517
_2503
Shewana3_2504: Shewana3_2504 - hypothetical protein (RefSeq), at 2,995,880 to 2,996,272
_2504
Shewana3_2505: Shewana3_2505 - thioesterase superfamily protein (RefSeq), at 2,996,403 to 2,996,867
_2505
Shewana3_2506: Shewana3_2506 - hypothetical protein (RefSeq), at 2,996,854 to 2,997,381
_2506
Shewana3_2507: Shewana3_2507 - histidine triad (HIT) protein (RefSeq), at 2,997,378 to 2,997,734
_2507
Position (kb)
2996
2997 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2995.504 kb on - strand at 2995.513 kb on + strand at 2995.521 kb on - strand at 2995.527 kb on - strand at 2995.632 kb on - strand at 2995.655 kb on - strand at 2995.683 kb on - strand at 2995.732 kb on - strand at 2995.871 kb on + strand at 2995.911 kb on - strand at 2996.354 kb on - strand at 2996.388 kb on + strand at 2996.798 kb on + strand, within Shewana3_2505 at 2996.806 kb on - strand, within Shewana3_2505 at 2996.953 kb on + strand, within Shewana3_2506 at 2996.987 kb on - strand, within Shewana3_2506 at 2996.992 kb on - strand, within Shewana3_2506 at 2997.019 kb on - strand, within Shewana3_2506 at 2997.052 kb on + strand, within Shewana3_2506 at 2997.194 kb on - strand, within Shewana3_2506 at 2997.569 kb on - strand, within Shewana3_2507 at 2997.848 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 2,995,504 - +1.7 2,995,513 + -0.3 2,995,521 - +0.9 2,995,527 - -1.2 2,995,632 - -1.6 2,995,655 - -0.6 2,995,683 - +1.1 2,995,732 - +2.2 2,995,871 + +0.2 2,995,911 - -0.3 2,996,354 - -0.0 2,996,388 + -0.2 2,996,798 + Shewana3_2505 0.85 -1.3 2,996,806 - Shewana3_2505 0.87 -1.1 2,996,953 + Shewana3_2506 0.19 -0.9 2,996,987 - Shewana3_2506 0.25 +0.7 2,996,992 - Shewana3_2506 0.26 -0.5 2,997,019 - Shewana3_2506 0.31 +1.4 2,997,052 + Shewana3_2506 0.38 -1.8 2,997,194 - Shewana3_2506 0.64 -0.4 2,997,569 - Shewana3_2507 0.54 -0.0 2,997,848 + -0.1
Or see this region's nucleotide sequence