Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2452 and Shewana3_2453 are separated by 163 nucleotides Shewana3_2453 and ushA are separated by 94 nucleotides ushA and Shewana3_2455 are separated by 364 nucleotides
Shewana3_2452: Shewana3_2452 - dihydrouridine synthase, DuS (RefSeq), at 2,940,208 to 2,941,155
_2452
Shewana3_2453: Shewana3_2453 - TraR/DksA family transcriptional regulator (RefSeq), at 2,941,319 to 2,941,678
_2453
Shewana3_2454: ushA - bifunctional UDP-sugar hydrolase/5'-nucleotidase periplasmic precursor (RefSeq), at 2,941,773 to 2,943,494
ushA
Shewana3_2455: Shewana3_2455 - phospholipase/carboxylesterase (RefSeq), at 2,943,859 to 2,944,524
_2455
Position (kb)
2941
2942
2943
2944 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2940.968 kb on + strand, within Shewana3_2452 at 2941.027 kb on - strand, within Shewana3_2452 at 2941.074 kb on + strand at 2941.172 kb on + strand at 2941.172 kb on + strand at 2941.288 kb on + strand at 2941.336 kb on - strand at 2941.443 kb on + strand, within Shewana3_2453 at 2941.538 kb on + strand, within Shewana3_2453 at 2941.610 kb on - strand, within Shewana3_2453 at 2941.787 kb on + strand at 2941.830 kb on + strand at 2941.956 kb on + strand, within ushA at 2942.069 kb on + strand, within ushA at 2942.272 kb on - strand, within ushA at 2942.324 kb on - strand, within ushA at 2942.456 kb on - strand, within ushA at 2942.491 kb on - strand, within ushA at 2942.643 kb on - strand, within ushA at 2942.725 kb on + strand, within ushA at 2942.742 kb on + strand, within ushA at 2942.790 kb on + strand, within ushA at 2943.092 kb on - strand, within ushA at 2943.180 kb on + strand, within ushA at 2943.389 kb on - strand at 2943.437 kb on - strand at 2943.475 kb on + strand at 2944.018 kb on - strand, within Shewana3_2455 at 2944.192 kb on + strand, within Shewana3_2455 at 2944.254 kb on + strand, within Shewana3_2455 at 2944.262 kb on - strand, within Shewana3_2455 at 2944.262 kb on - strand, within Shewana3_2455 at 2944.265 kb on + strand, within Shewana3_2455 at 2944.281 kb on - strand, within Shewana3_2455 at 2944.398 kb on - strand, within Shewana3_2455
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 2,940,968 + Shewana3_2452 0.80 +0.0 2,941,027 - Shewana3_2452 0.86 -0.3 2,941,074 + -0.8 2,941,172 + +1.3 2,941,172 + +1.6 2,941,288 + +0.7 2,941,336 - +0.9 2,941,443 + Shewana3_2453 0.34 +0.3 2,941,538 + Shewana3_2453 0.61 -0.3 2,941,610 - Shewana3_2453 0.81 +0.4 2,941,787 + +1.6 2,941,830 + +1.4 2,941,956 + ushA Shewana3_2454 0.11 +0.6 2,942,069 + ushA Shewana3_2454 0.17 +3.2 2,942,272 - ushA Shewana3_2454 0.29 +0.8 2,942,324 - ushA Shewana3_2454 0.32 +0.3 2,942,456 - ushA Shewana3_2454 0.40 -0.0 2,942,491 - ushA Shewana3_2454 0.42 +0.9 2,942,643 - ushA Shewana3_2454 0.51 +0.4 2,942,725 + ushA Shewana3_2454 0.55 +0.9 2,942,742 + ushA Shewana3_2454 0.56 +0.9 2,942,790 + ushA Shewana3_2454 0.59 +2.2 2,943,092 - ushA Shewana3_2454 0.77 +0.2 2,943,180 + ushA Shewana3_2454 0.82 -0.7 2,943,389 - +1.6 2,943,437 - +0.3 2,943,475 + +2.2 2,944,018 - Shewana3_2455 0.24 +1.9 2,944,192 + Shewana3_2455 0.50 +0.2 2,944,254 + Shewana3_2455 0.59 +3.2 2,944,262 - Shewana3_2455 0.61 -0.4 2,944,262 - Shewana3_2455 0.61 -0.6 2,944,265 + Shewana3_2455 0.61 +3.2 2,944,281 - Shewana3_2455 0.63 +0.6 2,944,398 - Shewana3_2455 0.81 +0.4
Or see this region's nucleotide sequence