Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2343 and Shewana3_2344 are separated by 70 nucleotides Shewana3_2344 and Shewana3_2345 are separated by 120 nucleotides Shewana3_2345 and Shewana3_2346 are separated by 113 nucleotides
Shewana3_2343: Shewana3_2343 - arsenite-activated ATPase ArsA (RefSeq), at 2,818,546 to 2,820,312
_2343
Shewana3_2344: Shewana3_2344 - arsenical pump membrane protein (RefSeq), at 2,820,383 to 2,821,666
_2344
Shewana3_2345: Shewana3_2345 - arsenate reductase (RefSeq), at 2,821,787 to 2,822,263
_2345
Shewana3_2346: Shewana3_2346 - acriflavin resistance protein (RefSeq), at 2,822,377 to 2,825,451
_2346
Position (kb)
2820
2821
2822 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2819.627 kb on - strand, within Shewana3_2343 at 2819.632 kb on - strand, within Shewana3_2343 at 2820.125 kb on + strand, within Shewana3_2343 at 2820.133 kb on - strand, within Shewana3_2343 at 2820.151 kb on + strand at 2820.167 kb on + strand at 2820.345 kb on + strand at 2820.443 kb on + strand at 2820.455 kb on + strand at 2820.479 kb on + strand at 2820.479 kb on + strand at 2820.788 kb on + strand, within Shewana3_2344 at 2820.788 kb on + strand, within Shewana3_2344 at 2820.788 kb on + strand, within Shewana3_2344 at 2820.857 kb on - strand, within Shewana3_2344 at 2821.076 kb on + strand, within Shewana3_2344 at 2821.233 kb on + strand, within Shewana3_2344 at 2821.278 kb on - strand, within Shewana3_2344 at 2821.312 kb on - strand, within Shewana3_2344 at 2821.329 kb on + strand, within Shewana3_2344 at 2821.465 kb on + strand, within Shewana3_2344 at 2821.640 kb on - strand at 2821.812 kb on - strand at 2821.850 kb on - strand, within Shewana3_2345 at 2821.869 kb on + strand, within Shewana3_2345 at 2822.036 kb on + strand, within Shewana3_2345 at 2822.140 kb on - strand, within Shewana3_2345 at 2822.327 kb on - strand at 2822.527 kb on + strand at 2822.549 kb on - strand at 2822.601 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 2,819,627 - Shewana3_2343 0.61 +0.5 2,819,632 - Shewana3_2343 0.61 +0.4 2,820,125 + Shewana3_2343 0.89 -0.5 2,820,133 - Shewana3_2343 0.90 +0.0 2,820,151 + +0.6 2,820,167 + +1.5 2,820,345 + +0.8 2,820,443 + +0.8 2,820,455 + +0.8 2,820,479 + +1.5 2,820,479 + +3.0 2,820,788 + Shewana3_2344 0.32 +3.1 2,820,788 + Shewana3_2344 0.32 -1.5 2,820,788 + Shewana3_2344 0.32 +0.4 2,820,857 - Shewana3_2344 0.37 -1.5 2,821,076 + Shewana3_2344 0.54 -2.0 2,821,233 + Shewana3_2344 0.66 -1.8 2,821,278 - Shewana3_2344 0.70 -0.1 2,821,312 - Shewana3_2344 0.72 -0.4 2,821,329 + Shewana3_2344 0.74 -0.0 2,821,465 + Shewana3_2344 0.84 -1.3 2,821,640 - -0.3 2,821,812 - +0.3 2,821,850 - Shewana3_2345 0.13 -1.9 2,821,869 + Shewana3_2345 0.17 +1.3 2,822,036 + Shewana3_2345 0.52 -0.6 2,822,140 - Shewana3_2345 0.74 +2.1 2,822,327 - -1.5 2,822,527 + +0.5 2,822,549 - -1.3 2,822,601 - -0.8
Or see this region's nucleotide sequence