Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_R0095 and Shewana3_R0096 are separated by 47 nucleotides Shewana3_R0096 and Shewana3_R0097 are separated by 31 nucleotides Shewana3_R0097 and Shewana3_R0098 are separated by 46 nucleotides Shewana3_R0098 and Shewana3_R0099 are separated by 46 nucleotides Shewana3_R0099 and Shewana3_2167 are separated by 208 nucleotides
Shewana3_R0095: Shewana3_R0095 - tRNA-Asn (RefSeq), at 2,597,882 to 2,597,957
_R0095
Shewana3_R0096: Shewana3_R0096 - tRNA-Asn (RefSeq), at 2,598,005 to 2,598,080
_R0096
Shewana3_R0097: Shewana3_R0097 - tRNA-Asn (RefSeq), at 2,598,112 to 2,598,187
_R0097
Shewana3_R0098: Shewana3_R0098 - tRNA-Asn (RefSeq), at 2,598,234 to 2,598,309
_R0098
Shewana3_R0099: Shewana3_R0099 - tRNA-Asn (RefSeq), at 2,598,356 to 2,598,431
_R0099
Shewana3_2167: Shewana3_2167 - diguanylate cyclase/phosphodiesterase (RefSeq), at 2,598,640 to 2,600,544
_2167
Position (kb)
2598
2599 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2597.310 kb on - strand at 2597.344 kb on - strand at 2597.359 kb on - strand at 2597.437 kb on + strand at 2598.296 kb on + strand, within Shewana3_R0098 at 2598.472 kb on - strand at 2598.887 kb on - strand, within Shewana3_2167 at 2599.181 kb on + strand, within Shewana3_2167
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 2,597,310 - +1.9 2,597,344 - -2.2 2,597,359 - +0.1 2,597,437 + +0.3 2,598,296 + Shewana3_R0098 0.82 +0.9 2,598,472 - +1.9 2,598,887 - Shewana3_2167 0.13 +1.3 2,599,181 + Shewana3_2167 0.28 +0.3
Or see this region's nucleotide sequence