Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2038 and Shewana3_R0086 are separated by 489 nucleotides Shewana3_R0086 and Shewana3_R0087 are separated by 38 nucleotides Shewana3_R0087 and Shewana3_2039 are separated by 251 nucleotides Shewana3_2039 and Shewana3_2040 are separated by 33 nucleotides Shewana3_2040 and Shewana3_2041 are separated by 9 nucleotides
Shewana3_2038: Shewana3_2038 - DEAD/DEAH box helicase domain-containing protein (RefSeq), at 2,440,874 to 2,447,236
_2038
Shewana3_R0086: Shewana3_R0086 - tRNA-Val (RefSeq), at 2,447,726 to 2,447,802
_R0086
Shewana3_R0087: Shewana3_R0087 - tRNA-Val (RefSeq), at 2,447,841 to 2,447,917
_R0087
Shewana3_2039: Shewana3_2039 - MATE efflux family protein (RefSeq), at 2,448,169 to 2,449,548
_2039
Shewana3_2040: Shewana3_2040 - riboflavin synthase subunit alpha (RefSeq), at 2,449,582 to 2,450,202
_2040
Shewana3_2041: Shewana3_2041 - hypothetical protein (RefSeq), at 2,450,212 to 2,450,412
_2041
Position (kb)
2448
2449
2450 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2447.263 kb on - strand at 2447.592 kb on - strand at 2447.942 kb on - strand at 2448.282 kb on - strand at 2448.744 kb on - strand, within Shewana3_2039 at 2448.876 kb on + strand, within Shewana3_2039 at 2448.980 kb on + strand, within Shewana3_2039 at 2449.422 kb on + strand at 2449.430 kb on - strand at 2449.607 kb on + strand at 2449.649 kb on + strand, within Shewana3_2040 at 2449.780 kb on + strand, within Shewana3_2040 at 2449.788 kb on - strand, within Shewana3_2040 at 2449.821 kb on - strand, within Shewana3_2040 at 2449.896 kb on - strand, within Shewana3_2040 at 2449.973 kb on + strand, within Shewana3_2040 at 2450.095 kb on - strand, within Shewana3_2040 at 2450.303 kb on - strand, within Shewana3_2041 at 2450.422 kb on - strand at 2450.473 kb on + strand at 2450.479 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 2,447,263 - +1.2 2,447,592 - +0.8 2,447,942 - +2.8 2,448,282 - -0.7 2,448,744 - Shewana3_2039 0.42 -1.6 2,448,876 + Shewana3_2039 0.51 +1.2 2,448,980 + Shewana3_2039 0.59 +0.2 2,449,422 + +0.9 2,449,430 - +0.3 2,449,607 + -0.7 2,449,649 + Shewana3_2040 0.11 -0.5 2,449,780 + Shewana3_2040 0.32 +0.4 2,449,788 - Shewana3_2040 0.33 +1.8 2,449,821 - Shewana3_2040 0.38 +1.8 2,449,896 - Shewana3_2040 0.51 +1.8 2,449,973 + Shewana3_2040 0.63 -0.3 2,450,095 - Shewana3_2040 0.83 +1.8 2,450,303 - Shewana3_2041 0.45 +1.2 2,450,422 - +0.8 2,450,473 + -0.4 2,450,479 - +0.2
Or see this region's nucleotide sequence