Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1985 and Shewana3_1986 are separated by 26 nucleotides Shewana3_1986 and Shewana3_1987 are separated by 391 nucleotides Shewana3_1987 and pssA are separated by 11 nucleotides
Shewana3_1985: Shewana3_1985 - D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase (RefSeq), at 2,363,811 to 2,365,352
_1985
Shewana3_1986: Shewana3_1986 - hypothetical protein (RefSeq), at 2,365,379 to 2,365,555
_1986
Shewana3_1987: Shewana3_1987 - hypothetical protein (RefSeq), at 2,365,947 to 2,366,279
_1987
Shewana3_1988: pssA - phosphatidylserine synthase (RefSeq), at 2,366,291 to 2,367,604
pssA
Position (kb)
2365
2366 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 2364.398 kb on + strand, within Shewana3_1985 at 2364.406 kb on - strand, within Shewana3_1985 at 2364.406 kb on - strand, within Shewana3_1985 at 2364.506 kb on + strand, within Shewana3_1985 at 2364.770 kb on - strand, within Shewana3_1985 at 2365.020 kb on - strand, within Shewana3_1985 at 2365.020 kb on - strand, within Shewana3_1985 at 2365.056 kb on - strand, within Shewana3_1985 at 2365.245 kb on + strand at 2365.253 kb on - strand at 2365.326 kb on - strand at 2365.355 kb on - strand at 2365.423 kb on + strand, within Shewana3_1986 at 2365.423 kb on + strand, within Shewana3_1986 at 2365.431 kb on - strand, within Shewana3_1986 at 2365.431 kb on - strand, within Shewana3_1986 at 2365.557 kb on + strand at 2365.647 kb on - strand at 2365.738 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 2,364,398 + Shewana3_1985 0.38 +2.9 2,364,406 - Shewana3_1985 0.39 +2.6 2,364,406 - Shewana3_1985 0.39 +0.3 2,364,506 + Shewana3_1985 0.45 +0.9 2,364,770 - Shewana3_1985 0.62 +2.9 2,365,020 - Shewana3_1985 0.78 +0.1 2,365,020 - Shewana3_1985 0.78 +6.0 2,365,056 - Shewana3_1985 0.81 -1.0 2,365,245 + -0.6 2,365,253 - +0.9 2,365,326 - +0.9 2,365,355 - +0.1 2,365,423 + Shewana3_1986 0.25 +0.1 2,365,423 + Shewana3_1986 0.25 -1.5 2,365,431 - Shewana3_1986 0.29 +1.3 2,365,431 - Shewana3_1986 0.29 +0.3 2,365,557 + +2.1 2,365,647 - +1.9 2,365,738 + +2.5
Or see this region's nucleotide sequence