Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1889 and Shewana3_1890 are separated by 123 nucleotides Shewana3_1890 and Shewana3_1891 overlap by 50 nucleotides Shewana3_1891 and Shewana3_1892 are separated by 312 nucleotides
Shewana3_1889: Shewana3_1889 - peptidoglycan binding domain-containing protein (RefSeq), at 2,238,829 to 2,240,505
_1889
Shewana3_1890: Shewana3_1890 - hypothetical protein (RefSeq), at 2,240,629 to 2,241,177
_1890
Shewana3_1891: Shewana3_1891 - hypothetical protein (RefSeq), at 2,241,128 to 2,241,562
_1891
Shewana3_1892: Shewana3_1892 - 50S ribosomal protein L25P (RefSeq), at 2,241,875 to 2,242,162
_1892
Position (kb) 2240
2241
2242 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6 at 2239.779 kb on + strand, within Shewana3_1889 at 2240.645 kb on - strand at 2240.645 kb on - strand at 2240.686 kb on - strand, within Shewana3_1890 at 2240.992 kb on + strand, within Shewana3_1890 at 2241.071 kb on + strand, within Shewana3_1890 at 2241.481 kb on - strand, within Shewana3_1891 at 2241.616 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 2,239,779 + Shewana3_1889 0.57 +1.3 2,240,645 - +2.9 2,240,645 - -0.3 2,240,686 - Shewana3_1890 0.10 +1.3 2,240,992 + Shewana3_1890 0.66 +6.4 2,241,071 + Shewana3_1890 0.81 +0.3 2,241,481 - Shewana3_1891 0.81 -0.1 2,241,616 + +0.6
Or see this region's nucleotide sequence