Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1847 and Shewana3_1848 are separated by 44 nucleotides Shewana3_1848 and Shewana3_1849 overlap by 19 nucleotides Shewana3_1849 and hisH are separated by 29 nucleotides hisH and Shewana3_1851 are separated by 145 nucleotides
Shewana3_1847: Shewana3_1847 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (RefSeq), at 2,189,352 to 2,190,011
_1847
Shewana3_1848: Shewana3_1848 - imidazole glycerol phosphate synthase subunit HisF (RefSeq), at 2,190,056 to 2,190,829
_1848
Shewana3_1849: Shewana3_1849 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (RefSeq), at 2,190,811 to 2,191,548
_1849
Shewana3_1850: hisH - imidazole glycerol phosphate synthase subunit HisH (RefSeq), at 2,191,578 to 2,192,228
hisH
Shewana3_1851: Shewana3_1851 - imidazole glycerol-phosphate dehydratase/histidinol phosphatase (RefSeq), at 2,192,374 to 2,193,465
_1851
Position (kb)
2190
2191
2192 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6
7 at 2189.996 kb on - strand at 2190.055 kb on - strand at 2190.406 kb on + strand, within Shewana3_1848 at 2190.633 kb on + strand, within Shewana3_1848 at 2190.634 kb on + strand, within Shewana3_1848 at 2190.641 kb on - strand, within Shewana3_1848 at 2190.778 kb on - strand at 2190.826 kb on + strand at 2190.981 kb on + strand, within Shewana3_1849 at 2190.989 kb on - strand, within Shewana3_1849 at 2191.094 kb on - strand, within Shewana3_1849 at 2191.651 kb on + strand, within hisH at 2191.714 kb on - strand, within hisH at 2191.845 kb on + strand, within hisH at 2191.980 kb on - strand, within hisH at 2191.997 kb on + strand, within hisH at 2192.006 kb on - strand, within hisH at 2192.122 kb on + strand, within hisH at 2192.137 kb on - strand, within hisH at 2192.473 kb on - strand at 2192.490 kb on - strand, within Shewana3_1851 at 2192.499 kb on + strand, within Shewana3_1851 at 2192.507 kb on - strand, within Shewana3_1851 at 2192.507 kb on - strand, within Shewana3_1851 at 2192.507 kb on - strand, within Shewana3_1851
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 2,189,996 - +0.6 2,190,055 - +7.2 2,190,406 + Shewana3_1848 0.45 -1.4 2,190,633 + Shewana3_1848 0.75 +2.3 2,190,634 + Shewana3_1848 0.75 +0.4 2,190,641 - Shewana3_1848 0.76 -0.6 2,190,778 - +0.6 2,190,826 + +2.0 2,190,981 + Shewana3_1849 0.23 +0.4 2,190,989 - Shewana3_1849 0.24 -0.7 2,191,094 - Shewana3_1849 0.38 -0.8 2,191,651 + hisH Shewana3_1850 0.11 +1.0 2,191,714 - hisH Shewana3_1850 0.21 +0.1 2,191,845 + hisH Shewana3_1850 0.41 -0.1 2,191,980 - hisH Shewana3_1850 0.62 +0.2 2,191,997 + hisH Shewana3_1850 0.64 +0.4 2,192,006 - hisH Shewana3_1850 0.66 -0.5 2,192,122 + hisH Shewana3_1850 0.84 +2.0 2,192,137 - hisH Shewana3_1850 0.86 +0.8 2,192,473 - -0.2 2,192,490 - Shewana3_1851 0.11 +0.3 2,192,499 + Shewana3_1851 0.11 +0.5 2,192,507 - Shewana3_1851 0.12 -0.9 2,192,507 - Shewana3_1851 0.12 +1.3 2,192,507 - Shewana3_1851 0.12 -1.0
Or see this region's nucleotide sequence