Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1432 and Shewana3_1433 are separated by 182 nucleotides Shewana3_1433 and queA are separated by 64 nucleotides queA and tgt are separated by 161 nucleotides
Shewana3_1432: Shewana3_1432 - hypothetical protein (RefSeq), at 1,662,021 to 1,662,647
_1432
Shewana3_1433: Shewana3_1433 - hypothetical protein (RefSeq), at 1,662,830 to 1,663,033
_1433
Shewana3_1434: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (RefSeq), at 1,663,098 to 1,664,135
queA
Shewana3_1435: tgt - queuine tRNA-ribosyltransferase (RefSeq), at 1,664,297 to 1,665,421
tgt
Position (kb)
1663
1664
1665 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1662.280 kb on + strand, within Shewana3_1432 at 1662.292 kb on + strand, within Shewana3_1432 at 1662.293 kb on - strand, within Shewana3_1432 at 1662.300 kb on - strand, within Shewana3_1432 at 1662.420 kb on + strand, within Shewana3_1432 at 1662.464 kb on - strand, within Shewana3_1432 at 1662.493 kb on + strand, within Shewana3_1432 at 1662.501 kb on - strand, within Shewana3_1432 at 1662.530 kb on - strand, within Shewana3_1432 at 1662.770 kb on + strand at 1663.090 kb on - strand at 1663.092 kb on - strand at 1663.179 kb on - strand at 1663.419 kb on - strand, within queA at 1663.432 kb on + strand, within queA at 1663.724 kb on + strand, within queA at 1663.732 kb on - strand, within queA at 1663.797 kb on + strand, within queA at 1664.046 kb on + strand at 1664.234 kb on - strand at 1664.512 kb on + strand, within tgt at 1664.552 kb on + strand, within tgt at 1664.771 kb on + strand, within tgt at 1664.785 kb on + strand, within tgt
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 1,662,280 + Shewana3_1432 0.41 -0.7 1,662,292 + Shewana3_1432 0.43 +1.4 1,662,293 - Shewana3_1432 0.43 -0.3 1,662,300 - Shewana3_1432 0.44 +0.7 1,662,420 + Shewana3_1432 0.64 -0.1 1,662,464 - Shewana3_1432 0.71 +1.8 1,662,493 + Shewana3_1432 0.75 -0.7 1,662,501 - Shewana3_1432 0.77 -0.6 1,662,530 - Shewana3_1432 0.81 +0.2 1,662,770 + +0.6 1,663,090 - +0.8 1,663,092 - -0.8 1,663,179 - -0.2 1,663,419 - queA Shewana3_1434 0.31 +1.1 1,663,432 + queA Shewana3_1434 0.32 +0.3 1,663,724 + queA Shewana3_1434 0.60 +0.8 1,663,732 - queA Shewana3_1434 0.61 +1.8 1,663,797 + queA Shewana3_1434 0.67 +0.9 1,664,046 + +0.2 1,664,234 - +3.1 1,664,512 + tgt Shewana3_1435 0.19 -0.3 1,664,552 + tgt Shewana3_1435 0.23 +0.4 1,664,771 + tgt Shewana3_1435 0.42 -0.2 1,664,785 + tgt Shewana3_1435 0.43 -1.9
Or see this region's nucleotide sequence