Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1244 and Shewana3_1245 are separated by 160 nucleotides Shewana3_1245 and Shewana3_1246 overlap by 20 nucleotides Shewana3_1246 and Shewana3_1247 are separated by 25 nucleotides Shewana3_1247 and Shewana3_1248 are separated by 13 nucleotides
Shewana3_1244: Shewana3_1244 - phage integrase family protein (RefSeq), at 1,458,630 to 1,459,829
_1244
Shewana3_1245: Shewana3_1245 - hypothetical protein (RefSeq), at 1,459,990 to 1,460,373
_1245
Shewana3_1246: Shewana3_1246 - transcriptional regulator-like protein (RefSeq), at 1,460,354 to 1,460,659
_1246
Shewana3_1247: Shewana3_1247 - hypothetical protein (RefSeq), at 1,460,685 to 1,461,503
_1247
Shewana3_1248: Shewana3_1248 - hypothetical protein (RefSeq), at 1,461,517 to 1,462,800
_1248
Position (kb) 1460
1461 Strain fitness (log2 ratio) -1
0
1
2
3
4
5
6
7
8 at 1459.390 kb on + strand, within Shewana3_1244 at 1459.390 kb on + strand, within Shewana3_1244 at 1459.453 kb on - strand, within Shewana3_1244 at 1459.563 kb on - strand, within Shewana3_1244 at 1459.570 kb on - strand, within Shewana3_1244 at 1459.798 kb on - strand at 1459.907 kb on + strand at 1460.157 kb on - strand, within Shewana3_1245 at 1460.854 kb on - strand, within Shewana3_1247 at 1460.982 kb on - strand, within Shewana3_1247 at 1461.091 kb on - strand, within Shewana3_1247 at 1461.225 kb on - strand, within Shewana3_1247 at 1461.356 kb on + strand, within Shewana3_1247 at 1461.356 kb on + strand, within Shewana3_1247 at 1461.364 kb on - strand, within Shewana3_1247 at 1461.409 kb on + strand, within Shewana3_1247 at 1461.498 kb on + strand at 1461.643 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 1,459,390 + Shewana3_1244 0.63 -0.2 1,459,390 + Shewana3_1244 0.63 -0.4 1,459,453 - Shewana3_1244 0.69 +0.1 1,459,563 - Shewana3_1244 0.78 -0.9 1,459,570 - Shewana3_1244 0.78 +0.3 1,459,798 - +0.8 1,459,907 + -1.5 1,460,157 - Shewana3_1245 0.43 +1.6 1,460,854 - Shewana3_1247 0.21 -0.5 1,460,982 - Shewana3_1247 0.36 +0.2 1,461,091 - Shewana3_1247 0.50 -1.5 1,461,225 - Shewana3_1247 0.66 +0.9 1,461,356 + Shewana3_1247 0.82 +1.0 1,461,356 + Shewana3_1247 0.82 +0.6 1,461,364 - Shewana3_1247 0.83 +1.2 1,461,409 + Shewana3_1247 0.88 +0.5 1,461,498 + -0.9 1,461,643 - +7.9
Or see this region's nucleotide sequence