Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1226 and Shewana3_1227 are separated by 32 nucleotides Shewana3_1227 and Shewana3_1228 overlap by 4 nucleotides Shewana3_1228 and ispG are separated by 42 nucleotides
Shewana3_1226: Shewana3_1226 - hypothetical protein (RefSeq), at 1,429,449 to 1,430,570
_1226
Shewana3_1227: Shewana3_1227 - type IV pilus biogenesis/stability protein PilW (RefSeq), at 1,430,603 to 1,431,391
_1227
Shewana3_1228: Shewana3_1228 - hypothetical protein (RefSeq), at 1,431,388 to 1,432,515
_1228
Shewana3_1229: ispG - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (RefSeq), at 1,432,558 to 1,433,673
ispG
Position (kb)
1431
1432
1433 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1430.821 kb on - strand, within Shewana3_1227 at 1430.925 kb on - strand, within Shewana3_1227 at 1431.499 kb on + strand at 1431.941 kb on + strand, within Shewana3_1228 at 1432.157 kb on + strand, within Shewana3_1228 at 1432.192 kb on + strand, within Shewana3_1228 at 1432.192 kb on + strand, within Shewana3_1228 at 1432.224 kb on + strand, within Shewana3_1228 at 1432.226 kb on + strand, within Shewana3_1228 at 1432.243 kb on + strand, within Shewana3_1228 at 1432.248 kb on + strand, within Shewana3_1228 at 1432.290 kb on + strand, within Shewana3_1228 at 1432.349 kb on + strand, within Shewana3_1228 at 1432.377 kb on + strand, within Shewana3_1228
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 1,430,821 - Shewana3_1227 0.28 +0.7 1,430,925 - Shewana3_1227 0.41 +1.3 1,431,499 + +0.1 1,431,941 + Shewana3_1228 0.49 +2.9 1,432,157 + Shewana3_1228 0.68 +2.5 1,432,192 + Shewana3_1228 0.71 +1.1 1,432,192 + Shewana3_1228 0.71 +0.3 1,432,224 + Shewana3_1228 0.74 +2.0 1,432,226 + Shewana3_1228 0.74 +2.0 1,432,243 + Shewana3_1228 0.76 +0.8 1,432,248 + Shewana3_1228 0.76 +2.0 1,432,290 + Shewana3_1228 0.80 +3.0 1,432,349 + Shewana3_1228 0.85 +3.1 1,432,377 + Shewana3_1228 0.88 +1.2
Or see this region's nucleotide sequence