Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0620 and Shewana3_0621 are separated by 205 nucleotides Shewana3_0621 and Shewana3_0622 are separated by 171 nucleotides Shewana3_0622 and Shewana3_0623 are separated by 77 nucleotides
Shewana3_0620: Shewana3_0620 - diguanylate cyclase/phosphodiesterase (RefSeq), at 724,932 to 727,118
_0620
Shewana3_0621: Shewana3_0621 - hypothetical protein (RefSeq), at 727,324 to 727,881
_0621
Shewana3_0622: Shewana3_0622 - ATP-dependent helicase HrpB (RefSeq), at 728,053 to 730,560
_0622
Shewana3_0623: Shewana3_0623 - penicillin-binding protein 1B (RefSeq), at 730,638 to 732,932
_0623
Position (kb) 728
729
730
731 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5 at 727.071 kb on - strand at 727.087 kb on - strand at 727.088 kb on - strand at 727.088 kb on - strand at 727.095 kb on - strand at 727.410 kb on - strand, within Shewana3_0621 at 727.504 kb on - strand, within Shewana3_0621 at 727.627 kb on + strand, within Shewana3_0621 at 727.637 kb on - strand, within Shewana3_0621 at 727.659 kb on - strand, within Shewana3_0621 at 727.814 kb on - strand, within Shewana3_0621 at 727.864 kb on - strand at 727.999 kb on + strand at 727.999 kb on + strand at 728.007 kb on - strand at 728.166 kb on - strand at 728.171 kb on - strand at 728.300 kb on - strand at 728.327 kb on - strand, within Shewana3_0622 at 728.434 kb on - strand, within Shewana3_0622 at 728.437 kb on + strand, within Shewana3_0622 at 728.476 kb on + strand, within Shewana3_0622 at 728.577 kb on + strand, within Shewana3_0622 at 728.579 kb on - strand, within Shewana3_0622 at 728.634 kb on + strand, within Shewana3_0622 at 728.754 kb on + strand, within Shewana3_0622 at 728.756 kb on + strand, within Shewana3_0622 at 728.756 kb on + strand, within Shewana3_0622 at 728.824 kb on + strand, within Shewana3_0622 at 728.824 kb on + strand, within Shewana3_0622 at 728.829 kb on + strand, within Shewana3_0622 at 728.837 kb on - strand, within Shewana3_0622 at 728.837 kb on - strand, within Shewana3_0622 at 728.980 kb on + strand, within Shewana3_0622 at 728.981 kb on + strand, within Shewana3_0622 at 728.987 kb on + strand, within Shewana3_0622 at 729.145 kb on + strand, within Shewana3_0622 at 729.145 kb on + strand, within Shewana3_0622 at 729.153 kb on - strand, within Shewana3_0622 at 729.153 kb on - strand, within Shewana3_0622 at 729.192 kb on + strand, within Shewana3_0622 at 729.265 kb on + strand, within Shewana3_0622 at 729.376 kb on + strand, within Shewana3_0622 at 729.376 kb on + strand, within Shewana3_0622 at 729.409 kb on - strand, within Shewana3_0622 at 729.463 kb on + strand, within Shewana3_0622 at 729.481 kb on - strand, within Shewana3_0622 at 729.481 kb on - strand, within Shewana3_0622 at 729.631 kb on + strand, within Shewana3_0622 at 729.679 kb on + strand, within Shewana3_0622 at 730.016 kb on - strand, within Shewana3_0622 at 730.234 kb on - strand, within Shewana3_0622 at 730.447 kb on - strand at 730.561 kb on + strand at 730.686 kb on - strand at 730.786 kb on + strand at 730.865 kb on - strand at 730.874 kb on + strand, within Shewana3_0623 at 730.901 kb on - strand, within Shewana3_0623 at 731.037 kb on + strand, within Shewana3_0623 at 731.231 kb on - strand, within Shewana3_0623 at 731.292 kb on - strand, within Shewana3_0623 at 731.299 kb on + strand, within Shewana3_0623 at 731.376 kb on + strand, within Shewana3_0623 at 731.384 kb on - strand, within Shewana3_0623 at 731.455 kb on - strand, within Shewana3_0623 at 731.482 kb on + strand, within Shewana3_0623 at 731.482 kb on + strand, within Shewana3_0623 at 731.482 kb on + strand, within Shewana3_0623 at 731.509 kb on - strand, within Shewana3_0623 at 731.526 kb on + strand, within Shewana3_0623
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 727,071 - +0.9 727,087 - -1.0 727,088 - +0.3 727,088 - -0.0 727,095 - -0.2 727,410 - Shewana3_0621 0.15 -0.2 727,504 - Shewana3_0621 0.32 +1.3 727,627 + Shewana3_0621 0.54 -0.0 727,637 - Shewana3_0621 0.56 -0.4 727,659 - Shewana3_0621 0.60 -1.5 727,814 - Shewana3_0621 0.88 -1.3 727,864 - -1.0 727,999 + -1.0 727,999 + +0.3 728,007 - -0.1 728,166 - -0.2 728,171 - +0.8 728,300 - +0.3 728,327 - Shewana3_0622 0.11 +0.3 728,434 - Shewana3_0622 0.15 -1.2 728,437 + Shewana3_0622 0.15 -0.2 728,476 + Shewana3_0622 0.17 +0.1 728,577 + Shewana3_0622 0.21 +0.0 728,579 - Shewana3_0622 0.21 -0.5 728,634 + Shewana3_0622 0.23 -0.2 728,754 + Shewana3_0622 0.28 +0.4 728,756 + Shewana3_0622 0.28 -0.9 728,756 + Shewana3_0622 0.28 -0.0 728,824 + Shewana3_0622 0.31 +0.1 728,824 + Shewana3_0622 0.31 -0.5 728,829 + Shewana3_0622 0.31 -2.3 728,837 - Shewana3_0622 0.31 -0.2 728,837 - Shewana3_0622 0.31 +0.0 728,980 + Shewana3_0622 0.37 -0.2 728,981 + Shewana3_0622 0.37 -0.1 728,987 + Shewana3_0622 0.37 -0.4 729,145 + Shewana3_0622 0.44 -0.1 729,145 + Shewana3_0622 0.44 -0.5 729,153 - Shewana3_0622 0.44 -1.1 729,153 - Shewana3_0622 0.44 -0.0 729,192 + Shewana3_0622 0.45 +5.2 729,265 + Shewana3_0622 0.48 +0.9 729,376 + Shewana3_0622 0.53 +0.2 729,376 + Shewana3_0622 0.53 +0.4 729,409 - Shewana3_0622 0.54 -0.2 729,463 + Shewana3_0622 0.56 +0.2 729,481 - Shewana3_0622 0.57 -0.9 729,481 - Shewana3_0622 0.57 +2.6 729,631 + Shewana3_0622 0.63 -0.0 729,679 + Shewana3_0622 0.65 +1.0 730,016 - Shewana3_0622 0.78 -1.0 730,234 - Shewana3_0622 0.87 -0.1 730,447 - -0.8 730,561 + -0.3 730,686 - -1.4 730,786 + -1.3 730,865 - -1.2 730,874 + Shewana3_0623 0.10 +2.1 730,901 - Shewana3_0623 0.11 +0.3 731,037 + Shewana3_0623 0.17 -0.4 731,231 - Shewana3_0623 0.26 -1.2 731,292 - Shewana3_0623 0.28 +1.3 731,299 + Shewana3_0623 0.29 -0.6 731,376 + Shewana3_0623 0.32 -0.3 731,384 - Shewana3_0623 0.33 -0.7 731,455 - Shewana3_0623 0.36 +2.0 731,482 + Shewana3_0623 0.37 -0.7 731,482 + Shewana3_0623 0.37 -0.4 731,482 + Shewana3_0623 0.37 -0.7 731,509 - Shewana3_0623 0.38 -1.7 731,526 + Shewana3_0623 0.39 -0.5
Or see this region's nucleotide sequence