Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0253 and Shewana3_0254 are separated by 302 nucleotides Shewana3_0254 and Shewana3_0255 are separated by 50 nucleotides Shewana3_0255 and Shewana3_0256 are separated by 38 nucleotides Shewana3_0256 and Shewana3_0257 are separated by 186 nucleotides
Shewana3_0253: Shewana3_0253 - two component transcriptional regulator (RefSeq), at 285,338 to 286,024
_0253
Shewana3_0254: Shewana3_0254 - protein of unknown function, Spy-related (RefSeq), at 286,327 to 286,818
_0254
Shewana3_0255: Shewana3_0255 - cation diffusion facilitator family transporter (RefSeq), at 286,869 to 287,768
_0255
Shewana3_0256: Shewana3_0256 - hypothetical protein (RefSeq), at 287,807 to 288,217
_0256
Shewana3_0257: Shewana3_0257 - outer membrane protein, putative (RefSeq), at 288,404 to 288,817
_0257
Position (kb) 286
287
288 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4
5
6 at 285.907 kb on + strand, within Shewana3_0253 at 285.907 kb on + strand, within Shewana3_0253 at 285.907 kb on + strand, within Shewana3_0253 at 285.915 kb on - strand, within Shewana3_0253 at 285.915 kb on - strand, within Shewana3_0253 at 285.915 kb on - strand, within Shewana3_0253 at 285.915 kb on - strand, within Shewana3_0253 at 285.927 kb on + strand, within Shewana3_0253 at 285.943 kb on + strand, within Shewana3_0253 at 286.049 kb on + strand at 286.058 kb on + strand at 286.125 kb on + strand at 286.195 kb on - strand at 286.226 kb on - strand at 286.282 kb on - strand at 286.286 kb on + strand at 286.434 kb on + strand, within Shewana3_0254 at 286.676 kb on + strand, within Shewana3_0254 at 286.751 kb on + strand, within Shewana3_0254 at 286.751 kb on + strand, within Shewana3_0254 at 286.812 kb on + strand at 286.850 kb on - strand at 286.947 kb on - strand at 286.967 kb on + strand, within Shewana3_0255 at 287.034 kb on + strand, within Shewana3_0255 at 287.107 kb on - strand, within Shewana3_0255 at 287.486 kb on - strand, within Shewana3_0255 at 287.494 kb on + strand, within Shewana3_0255 at 287.502 kb on - strand, within Shewana3_0255 at 287.589 kb on - strand, within Shewana3_0255 at 287.599 kb on - strand, within Shewana3_0255 at 287.719 kb on - strand at 287.744 kb on + strand at 287.744 kb on + strand at 287.809 kb on + strand at 287.841 kb on - strand at 287.886 kb on + strand, within Shewana3_0256 at 287.921 kb on + strand, within Shewana3_0256 at 287.988 kb on + strand, within Shewana3_0256 at 287.988 kb on + strand, within Shewana3_0256 at 287.988 kb on + strand, within Shewana3_0256 at 287.988 kb on + strand, within Shewana3_0256 at 287.988 kb on + strand, within Shewana3_0256 at 287.988 kb on + strand, within Shewana3_0256 at 287.988 kb on + strand, within Shewana3_0256 at 287.996 kb on - strand, within Shewana3_0256 at 287.996 kb on - strand, within Shewana3_0256 at 287.996 kb on - strand, within Shewana3_0256 at 287.996 kb on - strand, within Shewana3_0256 at 287.996 kb on - strand, within Shewana3_0256 at 287.996 kb on - strand, within Shewana3_0256 at 288.055 kb on - strand, within Shewana3_0256 at 288.093 kb on + strand, within Shewana3_0256 at 288.101 kb on - strand, within Shewana3_0256 at 288.101 kb on - strand, within Shewana3_0256 at 288.102 kb on + strand, within Shewana3_0256 at 288.102 kb on + strand, within Shewana3_0256 at 288.110 kb on - strand, within Shewana3_0256 at 288.138 kb on + strand, within Shewana3_0256 at 288.157 kb on - strand, within Shewana3_0256 at 288.157 kb on - strand, within Shewana3_0256 at 288.384 kb on - strand at 288.389 kb on - strand at 288.460 kb on + strand, within Shewana3_0257 at 288.473 kb on + strand, within Shewana3_0257 at 288.518 kb on + strand, within Shewana3_0257 at 288.519 kb on + strand, within Shewana3_0257 at 288.526 kb on - strand, within Shewana3_0257 at 288.526 kb on - strand, within Shewana3_0257 at 288.528 kb on - strand, within Shewana3_0257 at 288.550 kb on - strand, within Shewana3_0257 at 288.562 kb on - strand, within Shewana3_0257 at 288.706 kb on + strand, within Shewana3_0257
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 285,907 + Shewana3_0253 0.83 +0.1 285,907 + Shewana3_0253 0.83 -0.9 285,907 + Shewana3_0253 0.83 +0.2 285,915 - Shewana3_0253 0.84 +0.1 285,915 - Shewana3_0253 0.84 +0.7 285,915 - Shewana3_0253 0.84 +1.1 285,915 - Shewana3_0253 0.84 -0.4 285,927 + Shewana3_0253 0.86 +0.3 285,943 + Shewana3_0253 0.88 -0.7 286,049 + -0.1 286,058 + -0.5 286,125 + +0.4 286,195 - +0.8 286,226 - -1.0 286,282 - -0.2 286,286 + -0.4 286,434 + Shewana3_0254 0.22 -1.1 286,676 + Shewana3_0254 0.71 +1.5 286,751 + Shewana3_0254 0.86 +0.7 286,751 + Shewana3_0254 0.86 +0.2 286,812 + +0.0 286,850 - +1.3 286,947 - +0.2 286,967 + Shewana3_0255 0.11 +0.4 287,034 + Shewana3_0255 0.18 +0.9 287,107 - Shewana3_0255 0.26 +1.1 287,486 - Shewana3_0255 0.69 +3.3 287,494 + Shewana3_0255 0.69 +0.2 287,502 - Shewana3_0255 0.70 +1.1 287,589 - Shewana3_0255 0.80 +0.1 287,599 - Shewana3_0255 0.81 +3.8 287,719 - -0.6 287,744 + -1.0 287,744 + +1.7 287,809 + +1.0 287,841 - -0.8 287,886 + Shewana3_0256 0.19 -0.3 287,921 + Shewana3_0256 0.28 -0.2 287,988 + Shewana3_0256 0.44 -1.9 287,988 + Shewana3_0256 0.44 -1.2 287,988 + Shewana3_0256 0.44 +0.3 287,988 + Shewana3_0256 0.44 -0.2 287,988 + Shewana3_0256 0.44 -0.5 287,988 + Shewana3_0256 0.44 -0.6 287,988 + Shewana3_0256 0.44 -0.3 287,996 - Shewana3_0256 0.46 +0.9 287,996 - Shewana3_0256 0.46 -1.6 287,996 - Shewana3_0256 0.46 -0.2 287,996 - Shewana3_0256 0.46 -0.1 287,996 - Shewana3_0256 0.46 -0.4 287,996 - Shewana3_0256 0.46 -1.0 288,055 - Shewana3_0256 0.60 -0.0 288,093 + Shewana3_0256 0.70 -0.6 288,101 - Shewana3_0256 0.72 -0.5 288,101 - Shewana3_0256 0.72 -0.8 288,102 + Shewana3_0256 0.72 -0.6 288,102 + Shewana3_0256 0.72 -0.0 288,110 - Shewana3_0256 0.74 +0.0 288,138 + Shewana3_0256 0.81 -0.3 288,157 - Shewana3_0256 0.85 -0.5 288,157 - Shewana3_0256 0.85 -0.1 288,384 - +6.3 288,389 - +0.2 288,460 + Shewana3_0257 0.14 -0.5 288,473 + Shewana3_0257 0.17 -0.4 288,518 + Shewana3_0257 0.28 -0.4 288,519 + Shewana3_0257 0.28 -0.8 288,526 - Shewana3_0257 0.29 -1.0 288,526 - Shewana3_0257 0.29 +1.1 288,528 - Shewana3_0257 0.30 -1.0 288,550 - Shewana3_0257 0.35 -1.6 288,562 - Shewana3_0257 0.38 +4.4 288,706 + Shewana3_0257 0.73 -1.5
Or see this region's nucleotide sequence