Experiment: Paraquat dichloride 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0028 and Shewana3_0029 are separated by 54 nucleotides Shewana3_0029 and Shewana3_0030 are separated by 198 nucleotides Shewana3_0030 and Shewana3_0031 are separated by 46 nucleotides
Shewana3_0028: Shewana3_0028 - protoporphyrinogen oxidase (RefSeq), at 35,517 to 36,041
_0028
Shewana3_0029: Shewana3_0029 - ArsR family transcriptional regulator (RefSeq), at 36,096 to 36,404
_0029
Shewana3_0030: Shewana3_0030 - protoporphyrinogen oxidase (RefSeq), at 36,603 to 37,157
_0030
Shewana3_0031: Shewana3_0031 - TrkH family potassium uptake protein (RefSeq), at 37,204 to 38,646
_0031
Position (kb) 36
37
38 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7 at 35.621 kb on - strand, within Shewana3_0028 at 35.631 kb on - strand, within Shewana3_0028 at 35.640 kb on + strand, within Shewana3_0028 at 35.640 kb on + strand, within Shewana3_0028 at 35.640 kb on + strand, within Shewana3_0028 at 35.641 kb on + strand, within Shewana3_0028 at 35.648 kb on - strand, within Shewana3_0028 at 35.649 kb on - strand, within Shewana3_0028 at 35.674 kb on - strand, within Shewana3_0028 at 35.742 kb on + strand, within Shewana3_0028 at 35.742 kb on + strand, within Shewana3_0028 at 35.750 kb on - strand, within Shewana3_0028 at 35.853 kb on - strand, within Shewana3_0028 at 35.925 kb on + strand, within Shewana3_0028 at 35.975 kb on - strand, within Shewana3_0028 at 36.060 kb on - strand at 36.060 kb on - strand at 36.078 kb on - strand at 36.092 kb on - strand at 36.115 kb on - strand at 36.159 kb on - strand, within Shewana3_0029 at 36.159 kb on - strand, within Shewana3_0029 at 36.159 kb on - strand, within Shewana3_0029 at 36.170 kb on - strand, within Shewana3_0029 at 36.170 kb on - strand, within Shewana3_0029 at 36.593 kb on + strand at 36.691 kb on + strand, within Shewana3_0030 at 36.696 kb on + strand, within Shewana3_0030 at 36.775 kb on + strand, within Shewana3_0030 at 36.884 kb on + strand, within Shewana3_0030 at 37.007 kb on - strand, within Shewana3_0030 at 37.008 kb on + strand, within Shewana3_0030 at 37.114 kb on - strand at 37.122 kb on + strand at 37.228 kb on + strand at 37.236 kb on - strand at 37.347 kb on + strand at 37.461 kb on + strand, within Shewana3_0031 at 37.461 kb on + strand, within Shewana3_0031 at 37.623 kb on - strand, within Shewana3_0031 at 37.739 kb on - strand, within Shewana3_0031 at 37.834 kb on + strand, within Shewana3_0031 at 38.063 kb on + strand, within Shewana3_0031
Per-strain Table
Position Strand Gene LocusTag Fraction Paraquat dichloride 0.008 mg/ml remove 35,621 - Shewana3_0028 0.20 +0.8 35,631 - Shewana3_0028 0.22 +1.2 35,640 + Shewana3_0028 0.23 +0.8 35,640 + Shewana3_0028 0.23 +1.6 35,640 + Shewana3_0028 0.23 +3.6 35,641 + Shewana3_0028 0.24 +1.1 35,648 - Shewana3_0028 0.25 +3.0 35,649 - Shewana3_0028 0.25 -0.4 35,674 - Shewana3_0028 0.30 +1.0 35,742 + Shewana3_0028 0.43 +1.5 35,742 + Shewana3_0028 0.43 +1.3 35,750 - Shewana3_0028 0.44 -0.1 35,853 - Shewana3_0028 0.64 +1.4 35,925 + Shewana3_0028 0.78 -0.4 35,975 - Shewana3_0028 0.87 +0.9 36,060 - +0.3 36,060 - -0.0 36,078 - -0.1 36,092 - +0.0 36,115 - -0.8 36,159 - Shewana3_0029 0.20 -0.2 36,159 - Shewana3_0029 0.20 -0.1 36,159 - Shewana3_0029 0.20 -0.4 36,170 - Shewana3_0029 0.24 -1.7 36,170 - Shewana3_0029 0.24 -1.5 36,593 + -2.0 36,691 + Shewana3_0030 0.16 +0.1 36,696 + Shewana3_0030 0.17 +0.2 36,775 + Shewana3_0030 0.31 +0.7 36,884 + Shewana3_0030 0.51 +0.6 37,007 - Shewana3_0030 0.73 -0.2 37,008 + Shewana3_0030 0.73 -0.8 37,114 - +0.0 37,122 + -0.0 37,228 + -3.2 37,236 - +7.1 37,347 + -1.0 37,461 + Shewana3_0031 0.18 -0.2 37,461 + Shewana3_0031 0.18 -0.9 37,623 - Shewana3_0031 0.29 -0.6 37,739 - Shewana3_0031 0.37 -0.7 37,834 + Shewana3_0031 0.44 -1.6 38,063 + Shewana3_0031 0.60 -0.6
Or see this region's nucleotide sequence