Experiment: nitrate 2000 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1959 and Shew_1960 are separated by 38 nucleotides Shew_1960 and Shew_1961 are separated by 162 nucleotides Shew_1961 and Shew_1962 are separated by 107 nucleotides
Shew_1959: Shew_1959 - short chain dehydrogenase (RefSeq), at 2,264,022 to 2,264,837
_1959
Shew_1960: Shew_1960 - hypothetical protein (RefSeq), at 2,264,876 to 2,265,418
_1960
Shew_1961: Shew_1961 - acyl-CoA dehydrogenase domain-containing protein (RefSeq), at 2,265,581 to 2,267,332
_1961
Shew_1962: Shew_1962 - D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase (RefSeq), at 2,267,440 to 2,269,071
_1962
Position (kb) 2265
2266
2267
2268 Strain fitness (log2 ratio) -1
0
1
2 at 2264.915 kb on - strand at 2265.246 kb on - strand, within Shew_1960 at 2265.783 kb on + strand, within Shew_1961 at 2266.095 kb on + strand, within Shew_1961 at 2266.496 kb on - strand, within Shew_1961 at 2267.254 kb on + strand at 2267.262 kb on - strand at 2267.897 kb on - strand, within Shew_1962 at 2267.978 kb on + strand, within Shew_1962
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 2000 mM remove 2,264,915 - +0.8 2,265,246 - Shew_1960 0.68 -0.6 2,265,783 + Shew_1961 0.12 +1.4 2,266,095 + Shew_1961 0.29 +1.5 2,266,496 - Shew_1961 0.52 +0.3 2,267,254 + +0.1 2,267,262 - -0.1 2,267,897 - Shew_1962 0.28 +1.9 2,267,978 + Shew_1962 0.33 +0.3
Or see this region's nucleotide sequence