Experiment: nitrate 2000 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1756 and Shew_1757 are separated by 2 nucleotides Shew_1757 and Shew_1758 overlap by 4 nucleotides Shew_1758 and Shew_1759 are separated by 2 nucleotides
Shew_1756: Shew_1756 - hydrogenase expression/formation protein HypE (RefSeq), at 2,006,941 to 2,007,951
_1756
Shew_1757: Shew_1757 - hydrogenase expression/formation protein HypD (RefSeq), at 2,007,954 to 2,009,069
_1757
Shew_1758: Shew_1758 - hydrogenase assembly chaperone hypC/hupF (RefSeq), at 2,009,066 to 2,009,314
_1758
Shew_1759: Shew_1759 - hydrogenase accessory protein HypB (RefSeq), at 2,009,317 to 2,010,150
_1759
Position (kb)
2007
2008
2009
2010 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2006.968 kb on - strand at 2007.120 kb on + strand, within Shew_1756 at 2007.195 kb on - strand, within Shew_1756 at 2007.969 kb on - strand at 2008.316 kb on - strand, within Shew_1757 at 2008.346 kb on + strand, within Shew_1757 at 2009.124 kb on - strand, within Shew_1758 at 2009.148 kb on + strand, within Shew_1758 at 2009.414 kb on + strand, within Shew_1759 at 2009.551 kb on + strand, within Shew_1759 at 2009.561 kb on + strand, within Shew_1759 at 2009.569 kb on - strand, within Shew_1759 at 2009.632 kb on + strand, within Shew_1759 at 2009.637 kb on + strand, within Shew_1759 at 2010.021 kb on + strand, within Shew_1759 at 2010.021 kb on + strand, within Shew_1759
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 2000 mM remove 2,006,968 - +0.9 2,007,120 + Shew_1756 0.18 +0.4 2,007,195 - Shew_1756 0.25 +0.0 2,007,969 - -0.0 2,008,316 - Shew_1757 0.32 -0.5 2,008,346 + Shew_1757 0.35 -1.4 2,009,124 - Shew_1758 0.23 +0.1 2,009,148 + Shew_1758 0.33 +0.6 2,009,414 + Shew_1759 0.12 +0.5 2,009,551 + Shew_1759 0.28 +3.3 2,009,561 + Shew_1759 0.29 -0.4 2,009,569 - Shew_1759 0.30 -0.1 2,009,632 + Shew_1759 0.38 -0.5 2,009,637 + Shew_1759 0.38 +1.4 2,010,021 + Shew_1759 0.84 -0.3 2,010,021 + Shew_1759 0.84 -0.2
Or see this region's nucleotide sequence