Experiment: nitrate 2000 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tolC and Shew_0545 are separated by 263 nucleotides Shew_0545 and Shew_0546 overlap by 4 nucleotides Shew_0546 and Shew_0547 are separated by 9 nucleotides
Shew_0544: tolC - outer membrane channel protein (RefSeq), at 614,493 to 615,839
tolC
Shew_0545: Shew_0545 - ADP-ribose diphosphatase (RefSeq), at 616,103 to 616,711
_0545
Shew_0546: Shew_0546 - hypothetical protein (RefSeq), at 616,708 to 617,154
_0546
Shew_0547: Shew_0547 - calcineurin phosphoesterase C-terminal domain-containing protein (RefSeq), at 617,164 to 618,003
_0547
Position (kb) 616
617
618 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 615.922 kb on + strand at 615.992 kb on + strand at 616.296 kb on + strand, within Shew_0545 at 616.304 kb on - strand, within Shew_0545 at 616.347 kb on + strand, within Shew_0545 at 616.414 kb on - strand, within Shew_0545 at 616.439 kb on - strand, within Shew_0545 at 616.700 kb on - strand at 616.781 kb on - strand, within Shew_0546 at 616.795 kb on + strand, within Shew_0546 at 617.142 kb on + strand at 617.689 kb on + strand, within Shew_0547
Per-strain Table
Position Strand Gene LocusTag Fraction nitrate 2000 mM remove 615,922 + -4.1 615,992 + -0.1 616,296 + Shew_0545 0.32 +0.4 616,304 - Shew_0545 0.33 +0.7 616,347 + Shew_0545 0.40 -0.2 616,414 - Shew_0545 0.51 +0.3 616,439 - Shew_0545 0.55 +1.0 616,700 - +0.8 616,781 - Shew_0546 0.16 +0.4 616,795 + Shew_0546 0.19 +1.2 617,142 + +1.7 617,689 + Shew_0547 0.62 +1.1
Or see this region's nucleotide sequence