Experiment: Thallium(I) acetate 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5013 and Pf1N1B4_5014 overlap by 4 nucleotides Pf1N1B4_5014 and Pf1N1B4_5015 are separated by 129 nucleotides
Pf1N1B4_5013 - Cointegrate resolution protein T, at 5,564,091 to 5,565,110
_5013
Pf1N1B4_5014 - Glutathione reductase (EC 1.8.1.7), at 5,565,107 to 5,566,465
_5014
Pf1N1B4_5015 - UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9), at 5,566,595 to 5,567,434
_5015
Position (kb) 5565
5566
5567 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5564.228 kb on + strand, within Pf1N1B4_5013 at 5564.794 kb on + strand, within Pf1N1B4_5013 at 5565.463 kb on - strand at 5565.546 kb on - strand, within Pf1N1B4_5014 at 5565.578 kb on - strand at 5565.578 kb on - strand, within Pf1N1B4_5014 at 5565.719 kb on - strand, within Pf1N1B4_5014 at 5565.719 kb on - strand, within Pf1N1B4_5014 at 5565.940 kb on - strand, within Pf1N1B4_5014 at 5566.443 kb on + strand at 5566.443 kb on + strand at 5566.443 kb on + strand at 5566.443 kb on + strand at 5566.443 kb on + strand at 5566.443 kb on + strand at 5566.444 kb on - strand at 5566.444 kb on - strand at 5566.458 kb on + strand at 5566.493 kb on + strand at 5566.493 kb on + strand at 5566.528 kb on + strand at 5566.528 kb on + strand at 5566.529 kb on - strand at 5566.529 kb on - strand at 5566.529 kb on - strand at 5566.599 kb on + strand at 5566.653 kb on + strand at 5566.654 kb on - strand at 5566.654 kb on - strand at 5566.654 kb on - strand at 5566.654 kb on - strand at 5566.698 kb on + strand, within Pf1N1B4_5015 at 5566.926 kb on + strand, within Pf1N1B4_5015 at 5566.926 kb on + strand, within Pf1N1B4_5015 at 5566.927 kb on - strand at 5566.990 kb on - strand, within Pf1N1B4_5015 at 5566.990 kb on - strand, within Pf1N1B4_5015 at 5566.990 kb on - strand, within Pf1N1B4_5015 at 5567.140 kb on - strand, within Pf1N1B4_5015 at 5567.140 kb on - strand, within Pf1N1B4_5015 at 5567.185 kb on - strand, within Pf1N1B4_5015 at 5567.185 kb on - strand, within Pf1N1B4_5015 at 5567.226 kb on + strand, within Pf1N1B4_5015 at 5567.227 kb on - strand, within Pf1N1B4_5015 at 5567.227 kb on - strand, within Pf1N1B4_5015 at 5567.434 kb on + strand at 5567.434 kb on + strand at 5567.434 kb on + strand at 5567.436 kb on + strand at 5567.436 kb on + strand at 5567.436 kb on + strand at 5567.437 kb on - strand at 5567.437 kb on - strand at 5567.437 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 0.008 mg/ml remove 5,564,228 + Pf1N1B4_5013 0.13 +0.5 5,564,794 + Pf1N1B4_5013 0.69 +0.1 5,565,463 - -2.5 5,565,546 - Pf1N1B4_5014 0.32 -1.5 5,565,578 - -2.1 5,565,578 - Pf1N1B4_5014 0.35 -1.1 5,565,719 - Pf1N1B4_5014 0.45 -0.7 5,565,719 - Pf1N1B4_5014 0.45 +1.1 5,565,940 - Pf1N1B4_5014 0.61 -0.5 5,566,443 + -0.5 5,566,443 + +0.2 5,566,443 + +1.1 5,566,443 + -2.1 5,566,443 + +0.8 5,566,443 + -1.1 5,566,444 - +0.3 5,566,444 - +0.5 5,566,458 + +1.1 5,566,493 + +0.7 5,566,493 + +0.5 5,566,528 + +0.5 5,566,528 + -0.4 5,566,529 - +0.8 5,566,529 - +0.5 5,566,529 - -1.1 5,566,599 + +0.5 5,566,653 + +1.5 5,566,654 - +1.5 5,566,654 - -0.5 5,566,654 - -0.9 5,566,654 - +0.5 5,566,698 + Pf1N1B4_5015 0.12 -0.3 5,566,926 + Pf1N1B4_5015 0.39 -0.5 5,566,926 + Pf1N1B4_5015 0.39 +0.5 5,566,927 - -0.1 5,566,990 - Pf1N1B4_5015 0.47 +0.2 5,566,990 - Pf1N1B4_5015 0.47 +0.1 5,566,990 - Pf1N1B4_5015 0.47 +3.1 5,567,140 - Pf1N1B4_5015 0.65 +1.1 5,567,140 - Pf1N1B4_5015 0.65 +1.5 5,567,185 - Pf1N1B4_5015 0.70 +0.6 5,567,185 - Pf1N1B4_5015 0.70 +0.5 5,567,226 + Pf1N1B4_5015 0.75 -0.4 5,567,227 - Pf1N1B4_5015 0.75 +1.3 5,567,227 - Pf1N1B4_5015 0.75 +0.8 5,567,434 + +0.3 5,567,434 + +0.6 5,567,434 + +1.1 5,567,436 + +0.9 5,567,436 + -0.3 5,567,436 + +0.4 5,567,437 - -1.3 5,567,437 - +1.5 5,567,437 - +0.2
Or see this region's nucleotide sequence