Experiment: Thallium(I) acetate 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2415 and Pf1N1B4_2416 are separated by 281 nucleotides Pf1N1B4_2416 and Pf1N1B4_2417 are separated by 170 nucleotides
Pf1N1B4_2415 - TldD family protein, Beta/Gamma-proteobacterial subgroup, at 2,736,823 to 2,738,265
_2415
Pf1N1B4_2416 - Choline dehydrogenase (EC 1.1.99.1), at 2,738,547 to 2,740,250
_2416
Pf1N1B4_2417 - Betaine aldehyde dehydrogenase (EC 1.2.1.8), at 2,740,421 to 2,741,893
_2417
Position (kb)
2738
2739
2740
2741 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2737.725 kb on - strand, within Pf1N1B4_2415 at 2737.725 kb on - strand, within Pf1N1B4_2415 at 2737.796 kb on + strand, within Pf1N1B4_2415 at 2737.892 kb on + strand, within Pf1N1B4_2415 at 2737.892 kb on + strand, within Pf1N1B4_2415 at 2737.892 kb on + strand, within Pf1N1B4_2415 at 2737.892 kb on + strand, within Pf1N1B4_2415 at 2737.940 kb on + strand, within Pf1N1B4_2415 at 2737.940 kb on + strand, within Pf1N1B4_2415 at 2737.940 kb on + strand, within Pf1N1B4_2415 at 2738.075 kb on + strand, within Pf1N1B4_2415 at 2738.189 kb on + strand at 2738.297 kb on + strand at 2738.297 kb on + strand at 2738.298 kb on - strand at 2738.298 kb on - strand at 2738.298 kb on - strand at 2738.298 kb on - strand at 2738.298 kb on - strand at 2738.298 kb on - strand at 2738.335 kb on + strand at 2738.340 kb on + strand at 2738.340 kb on + strand at 2738.341 kb on - strand at 2738.358 kb on - strand at 2738.378 kb on + strand at 2738.540 kb on - strand at 2738.540 kb on - strand at 2738.540 kb on - strand at 2738.548 kb on + strand at 2738.611 kb on + strand at 2738.612 kb on - strand at 2738.612 kb on - strand at 2738.612 kb on - strand at 2738.612 kb on - strand at 2738.612 kb on - strand at 2738.614 kb on + strand at 2738.614 kb on + strand at 2738.615 kb on - strand at 2738.615 kb on - strand at 2739.004 kb on + strand, within Pf1N1B4_2416 at 2739.005 kb on - strand, within Pf1N1B4_2416 at 2739.005 kb on - strand, within Pf1N1B4_2416 at 2739.005 kb on - strand, within Pf1N1B4_2416 at 2739.005 kb on - strand, within Pf1N1B4_2416 at 2739.005 kb on - strand, within Pf1N1B4_2416 at 2739.133 kb on + strand, within Pf1N1B4_2416 at 2739.133 kb on + strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.134 kb on - strand, within Pf1N1B4_2416 at 2739.138 kb on - strand, within Pf1N1B4_2416 at 2739.138 kb on - strand, within Pf1N1B4_2416 at 2739.329 kb on - strand, within Pf1N1B4_2416 at 2739.329 kb on - strand, within Pf1N1B4_2416 at 2739.329 kb on - strand, within Pf1N1B4_2416 at 2739.329 kb on - strand, within Pf1N1B4_2416 at 2739.337 kb on + strand, within Pf1N1B4_2416 at 2739.337 kb on + strand, within Pf1N1B4_2416 at 2739.536 kb on - strand, within Pf1N1B4_2416 at 2739.875 kb on - strand, within Pf1N1B4_2416 at 2739.958 kb on + strand, within Pf1N1B4_2416 at 2739.958 kb on + strand, within Pf1N1B4_2416 at 2739.958 kb on + strand, within Pf1N1B4_2416 at 2739.959 kb on - strand, within Pf1N1B4_2416 at 2739.959 kb on - strand, within Pf1N1B4_2416 at 2739.959 kb on - strand, within Pf1N1B4_2416 at 2739.959 kb on - strand, within Pf1N1B4_2416 at 2739.959 kb on - strand, within Pf1N1B4_2416 at 2739.959 kb on - strand, within Pf1N1B4_2416 at 2740.055 kb on - strand, within Pf1N1B4_2416 at 2740.231 kb on + strand at 2740.231 kb on + strand at 2740.231 kb on + strand at 2740.232 kb on - strand at 2740.414 kb on + strand at 2740.414 kb on + strand at 2740.414 kb on + strand at 2740.415 kb on - strand at 2740.422 kb on + strand at 2740.423 kb on - strand at 2740.423 kb on - strand at 2740.702 kb on - strand, within Pf1N1B4_2417 at 2740.876 kb on - strand, within Pf1N1B4_2417 at 2741.055 kb on + strand, within Pf1N1B4_2417 at 2741.055 kb on + strand, within Pf1N1B4_2417
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 0.008 mg/ml remove 2,737,725 - Pf1N1B4_2415 0.63 +0.6 2,737,725 - Pf1N1B4_2415 0.63 -0.8 2,737,796 + Pf1N1B4_2415 0.67 +0.3 2,737,892 + Pf1N1B4_2415 0.74 +0.3 2,737,892 + Pf1N1B4_2415 0.74 +1.0 2,737,892 + Pf1N1B4_2415 0.74 -0.1 2,737,892 + Pf1N1B4_2415 0.74 -0.7 2,737,940 + Pf1N1B4_2415 0.77 -0.7 2,737,940 + Pf1N1B4_2415 0.77 +0.1 2,737,940 + Pf1N1B4_2415 0.77 -2.0 2,738,075 + Pf1N1B4_2415 0.87 +1.3 2,738,189 + +1.6 2,738,297 + +0.2 2,738,297 + +1.3 2,738,298 - +0.9 2,738,298 - +0.3 2,738,298 - -0.2 2,738,298 - +0.1 2,738,298 - -0.4 2,738,298 - +0.3 2,738,335 + -1.7 2,738,340 + -1.1 2,738,340 + +0.3 2,738,341 - -0.3 2,738,358 - -1.3 2,738,378 + -0.9 2,738,540 - -0.7 2,738,540 - -0.5 2,738,540 - -0.7 2,738,548 + -0.6 2,738,611 + -0.7 2,738,612 - +0.3 2,738,612 - -2.3 2,738,612 - -1.3 2,738,612 - -1.3 2,738,612 - -2.7 2,738,614 + -1.5 2,738,614 + -0.7 2,738,615 - -1.5 2,738,615 - -1.3 2,739,004 + Pf1N1B4_2416 0.27 -0.7 2,739,005 - Pf1N1B4_2416 0.27 -2.7 2,739,005 - Pf1N1B4_2416 0.27 -2.5 2,739,005 - Pf1N1B4_2416 0.27 -2.7 2,739,005 - Pf1N1B4_2416 0.27 -2.0 2,739,005 - Pf1N1B4_2416 0.27 -0.7 2,739,133 + Pf1N1B4_2416 0.34 -0.4 2,739,133 + Pf1N1B4_2416 0.34 -1.9 2,739,134 - Pf1N1B4_2416 0.34 -1.9 2,739,134 - Pf1N1B4_2416 0.34 -0.4 2,739,134 - Pf1N1B4_2416 0.34 -2.6 2,739,134 - Pf1N1B4_2416 0.34 -2.0 2,739,134 - Pf1N1B4_2416 0.34 +0.6 2,739,134 - Pf1N1B4_2416 0.34 -1.7 2,739,138 - Pf1N1B4_2416 0.35 -1.7 2,739,138 - Pf1N1B4_2416 0.35 -2.0 2,739,329 - Pf1N1B4_2416 0.46 -0.0 2,739,329 - Pf1N1B4_2416 0.46 -1.4 2,739,329 - Pf1N1B4_2416 0.46 -2.5 2,739,329 - Pf1N1B4_2416 0.46 -0.8 2,739,337 + Pf1N1B4_2416 0.46 -2.3 2,739,337 + Pf1N1B4_2416 0.46 -2.0 2,739,536 - Pf1N1B4_2416 0.58 -1.3 2,739,875 - Pf1N1B4_2416 0.78 -1.7 2,739,958 + Pf1N1B4_2416 0.83 -3.1 2,739,958 + Pf1N1B4_2416 0.83 -0.7 2,739,958 + Pf1N1B4_2416 0.83 -1.7 2,739,959 - Pf1N1B4_2416 0.83 -0.7 2,739,959 - Pf1N1B4_2416 0.83 -1.0 2,739,959 - Pf1N1B4_2416 0.83 -0.7 2,739,959 - Pf1N1B4_2416 0.83 -1.7 2,739,959 - Pf1N1B4_2416 0.83 -1.5 2,739,959 - Pf1N1B4_2416 0.83 -1.0 2,740,055 - Pf1N1B4_2416 0.88 -3.4 2,740,231 + -0.4 2,740,231 + -2.3 2,740,231 + -1.7 2,740,232 - -1.8 2,740,414 + -0.7 2,740,414 + -1.3 2,740,414 + -1.7 2,740,415 - -0.9 2,740,422 + -2.0 2,740,423 - +0.3 2,740,423 - +0.7 2,740,702 - Pf1N1B4_2417 0.19 -1.3 2,740,876 - Pf1N1B4_2417 0.31 -0.7 2,741,055 + Pf1N1B4_2417 0.43 -1.3 2,741,055 + Pf1N1B4_2417 0.43 -0.7
Or see this region's nucleotide sequence