Experiment: Thallium(I) acetate 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2046 and Pf1N1B4_2047 overlap by 1 nucleotides Pf1N1B4_2047 and Pf1N1B4_2048 are separated by 186 nucleotides Pf1N1B4_2048 and Pf1N1B4_2049 overlap by 4 nucleotides Pf1N1B4_2049 and Pf1N1B4_2050 are separated by 19 nucleotides Pf1N1B4_2050 and Pf1N1B4_2051 are separated by 192 nucleotides Pf1N1B4_2051 and Pf1N1B4_2052 are separated by 99 nucleotides
Pf1N1B4_2046 - 4-hydroxybenzoate polyprenyltransferase (EC 2.5.1.39), at 2,334,561 to 2,335,451
_2046
Pf1N1B4_2047 - Chorismate--pyruvate lyase (EC 4.1.3.40), at 2,335,451 to 2,335,885
_2047
Pf1N1B4_2048 - hypothetical protein, at 2,336,072 to 2,336,218
_2048
Pf1N1B4_2049 - Rubredoxin, at 2,336,215 to 2,336,382
_2049
Pf1N1B4_2050 - Rubredoxin-NAD(+) reductase (EC 1.18.1.1), at 2,336,402 to 2,337,550
_2050
Pf1N1B4_2051 - DNA-binding protein HU-alpha, at 2,337,743 to 2,338,018
_2051
Pf1N1B4_2052 - hypothetical protein, at 2,338,118 to 2,338,327
_2052
Position (kb)
2336
2337
2338 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2335.687 kb on + strand, within Pf1N1B4_2047 at 2335.687 kb on + strand, within Pf1N1B4_2047 at 2335.687 kb on + strand, within Pf1N1B4_2047 at 2335.687 kb on + strand, within Pf1N1B4_2047 at 2335.687 kb on + strand, within Pf1N1B4_2047 at 2335.767 kb on + strand, within Pf1N1B4_2047 at 2335.767 kb on + strand, within Pf1N1B4_2047 at 2335.767 kb on + strand, within Pf1N1B4_2047 at 2335.767 kb on + strand, within Pf1N1B4_2047 at 2335.767 kb on + strand, within Pf1N1B4_2047 at 2335.768 kb on - strand, within Pf1N1B4_2047 at 2335.768 kb on - strand at 2335.922 kb on + strand at 2335.922 kb on + strand at 2335.936 kb on + strand at 2335.936 kb on + strand at 2335.936 kb on + strand at 2335.936 kb on + strand at 2335.936 kb on + strand at 2335.937 kb on - strand at 2336.052 kb on + strand at 2336.052 kb on + strand at 2336.052 kb on + strand at 2336.052 kb on + strand at 2336.052 kb on + strand at 2336.052 kb on + strand at 2336.053 kb on - strand at 2336.130 kb on + strand, within Pf1N1B4_2048 at 2336.130 kb on + strand, within Pf1N1B4_2048 at 2336.130 kb on + strand, within Pf1N1B4_2048 at 2336.130 kb on + strand, within Pf1N1B4_2048 at 2336.208 kb on + strand at 2336.209 kb on - strand at 2336.209 kb on - strand at 2336.251 kb on + strand, within Pf1N1B4_2049 at 2336.251 kb on + strand, within Pf1N1B4_2049 at 2336.252 kb on - strand at 2336.252 kb on - strand, within Pf1N1B4_2049 at 2336.252 kb on - strand at 2336.252 kb on - strand, within Pf1N1B4_2049 at 2336.380 kb on + strand at 2336.380 kb on + strand at 2336.380 kb on + strand at 2336.381 kb on - strand at 2336.381 kb on - strand at 2336.381 kb on - strand at 2336.381 kb on - strand at 2336.522 kb on + strand, within Pf1N1B4_2050 at 2336.560 kb on + strand, within Pf1N1B4_2050 at 2336.560 kb on + strand, within Pf1N1B4_2050 at 2336.560 kb on + strand, within Pf1N1B4_2050 at 2336.665 kb on + strand, within Pf1N1B4_2050 at 2336.699 kb on + strand, within Pf1N1B4_2050 at 2336.699 kb on + strand at 2336.699 kb on + strand, within Pf1N1B4_2050 at 2336.700 kb on - strand, within Pf1N1B4_2050 at 2336.700 kb on - strand, within Pf1N1B4_2050 at 2336.798 kb on + strand, within Pf1N1B4_2050 at 2336.799 kb on - strand, within Pf1N1B4_2050 at 2336.901 kb on - strand, within Pf1N1B4_2050 at 2337.096 kb on + strand, within Pf1N1B4_2050 at 2337.096 kb on + strand, within Pf1N1B4_2050 at 2337.096 kb on + strand, within Pf1N1B4_2050 at 2337.096 kb on + strand, within Pf1N1B4_2050 at 2337.096 kb on + strand, within Pf1N1B4_2050 at 2337.097 kb on - strand, within Pf1N1B4_2050 at 2337.097 kb on - strand, within Pf1N1B4_2050 at 2337.097 kb on - strand, within Pf1N1B4_2050 at 2337.097 kb on - strand, within Pf1N1B4_2050 at 2337.097 kb on - strand, within Pf1N1B4_2050 at 2337.097 kb on - strand, within Pf1N1B4_2050 at 2337.097 kb on - strand, within Pf1N1B4_2050 at 2337.097 kb on - strand, within Pf1N1B4_2050 at 2337.212 kb on + strand, within Pf1N1B4_2050 at 2337.212 kb on + strand, within Pf1N1B4_2050 at 2337.257 kb on + strand, within Pf1N1B4_2050 at 2337.257 kb on + strand, within Pf1N1B4_2050 at 2337.257 kb on + strand, within Pf1N1B4_2050 at 2337.257 kb on + strand, within Pf1N1B4_2050 at 2337.257 kb on + strand, within Pf1N1B4_2050 at 2337.257 kb on + strand, within Pf1N1B4_2050 at 2337.257 kb on + strand, within Pf1N1B4_2050 at 2337.258 kb on - strand, within Pf1N1B4_2050 at 2337.258 kb on - strand, within Pf1N1B4_2050 at 2337.258 kb on - strand, within Pf1N1B4_2050 at 2337.258 kb on - strand, within Pf1N1B4_2050 at 2337.317 kb on - strand, within Pf1N1B4_2050 at 2337.317 kb on - strand, within Pf1N1B4_2050 at 2337.329 kb on + strand, within Pf1N1B4_2050 at 2337.472 kb on - strand at 2337.479 kb on + strand at 2337.548 kb on + strand at 2337.549 kb on - strand at 2337.552 kb on + strand at 2337.622 kb on - strand at 2337.659 kb on + strand at 2337.710 kb on + strand at 2337.710 kb on + strand at 2337.711 kb on - strand at 2337.711 kb on - strand at 2337.711 kb on - strand at 2337.711 kb on - strand at 2337.711 kb on - strand at 2337.741 kb on + strand at 2337.741 kb on + strand at 2337.741 kb on + strand at 2337.748 kb on + strand at 2338.016 kb on + strand at 2338.139 kb on + strand, within Pf1N1B4_2052 at 2338.179 kb on + strand, within Pf1N1B4_2052 at 2338.179 kb on + strand, within Pf1N1B4_2052 at 2338.180 kb on - strand, within Pf1N1B4_2052 at 2338.180 kb on - strand, within Pf1N1B4_2052 at 2338.257 kb on + strand, within Pf1N1B4_2052 at 2338.257 kb on + strand, within Pf1N1B4_2052 at 2338.258 kb on - strand, within Pf1N1B4_2052 at 2338.258 kb on - strand, within Pf1N1B4_2052 at 2338.258 kb on - strand, within Pf1N1B4_2052 at 2338.266 kb on + strand, within Pf1N1B4_2052 at 2338.281 kb on - strand, within Pf1N1B4_2052 at 2338.281 kb on - strand, within Pf1N1B4_2052 at 2338.281 kb on - strand, within Pf1N1B4_2052 at 2338.281 kb on - strand, within Pf1N1B4_2052 at 2338.281 kb on - strand, within Pf1N1B4_2052 at 2338.361 kb on + strand at 2338.362 kb on - strand at 2338.415 kb on + strand at 2338.416 kb on - strand at 2338.416 kb on - strand at 2338.416 kb on - strand at 2338.416 kb on - strand at 2338.416 kb on - strand at 2338.416 kb on - strand at 2338.461 kb on + strand at 2338.461 kb on + strand at 2338.461 kb on + strand at 2338.462 kb on - strand at 2338.462 kb on - strand at 2338.462 kb on - strand at 2338.462 kb on - strand at 2338.462 kb on - strand at 2338.462 kb on - strand at 2338.462 kb on - strand at 2338.495 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 0.008 mg/ml remove 2,335,687 + Pf1N1B4_2047 0.54 +0.5 2,335,687 + Pf1N1B4_2047 0.54 +2.3 2,335,687 + Pf1N1B4_2047 0.54 +1.8 2,335,687 + Pf1N1B4_2047 0.54 +1.0 2,335,687 + Pf1N1B4_2047 0.54 +2.2 2,335,767 + Pf1N1B4_2047 0.73 +2.0 2,335,767 + Pf1N1B4_2047 0.73 +2.6 2,335,767 + Pf1N1B4_2047 0.73 +0.5 2,335,767 + Pf1N1B4_2047 0.73 +0.5 2,335,767 + Pf1N1B4_2047 0.73 -0.3 2,335,768 - Pf1N1B4_2047 0.73 -0.2 2,335,768 - +1.5 2,335,922 + +3.3 2,335,922 + +0.2 2,335,936 + +2.6 2,335,936 + +2.5 2,335,936 + +2.3 2,335,936 + +3.1 2,335,936 + +1.3 2,335,937 - -3.2 2,336,052 + +1.8 2,336,052 + +0.8 2,336,052 + +1.1 2,336,052 + +1.4 2,336,052 + +1.9 2,336,052 + +1.1 2,336,053 - -0.9 2,336,130 + Pf1N1B4_2048 0.39 -0.3 2,336,130 + Pf1N1B4_2048 0.39 -1.5 2,336,130 + Pf1N1B4_2048 0.39 +0.7 2,336,130 + Pf1N1B4_2048 0.39 +0.5 2,336,208 + +1.1 2,336,209 - -0.3 2,336,209 - +0.5 2,336,251 + Pf1N1B4_2049 0.21 -0.8 2,336,251 + Pf1N1B4_2049 0.21 +0.5 2,336,252 - -1.1 2,336,252 - Pf1N1B4_2049 0.22 -0.8 2,336,252 - +0.5 2,336,252 - Pf1N1B4_2049 0.22 +0.9 2,336,380 + +0.5 2,336,380 + -0.9 2,336,380 + -0.5 2,336,381 - -2.5 2,336,381 - -1.9 2,336,381 - +1.5 2,336,381 - +0.1 2,336,522 + Pf1N1B4_2050 0.10 -1.1 2,336,560 + Pf1N1B4_2050 0.14 +2.5 2,336,560 + Pf1N1B4_2050 0.14 -0.4 2,336,560 + Pf1N1B4_2050 0.14 -0.5 2,336,665 + Pf1N1B4_2050 0.23 -0.1 2,336,699 + Pf1N1B4_2050 0.26 +1.5 2,336,699 + -0.5 2,336,699 + Pf1N1B4_2050 0.26 -0.3 2,336,700 - Pf1N1B4_2050 0.26 +0.6 2,336,700 - Pf1N1B4_2050 0.26 -1.5 2,336,798 + Pf1N1B4_2050 0.34 +0.1 2,336,799 - Pf1N1B4_2050 0.35 +0.0 2,336,901 - Pf1N1B4_2050 0.43 -0.1 2,337,096 + Pf1N1B4_2050 0.60 -0.1 2,337,096 + Pf1N1B4_2050 0.60 -0.1 2,337,096 + Pf1N1B4_2050 0.60 -0.9 2,337,096 + Pf1N1B4_2050 0.60 -1.1 2,337,096 + Pf1N1B4_2050 0.60 -2.5 2,337,097 - Pf1N1B4_2050 0.60 -0.3 2,337,097 - Pf1N1B4_2050 0.60 -0.5 2,337,097 - Pf1N1B4_2050 0.60 +0.5 2,337,097 - Pf1N1B4_2050 0.60 -1.1 2,337,097 - Pf1N1B4_2050 0.60 -1.9 2,337,097 - Pf1N1B4_2050 0.60 -0.5 2,337,097 - Pf1N1B4_2050 0.60 -0.2 2,337,097 - Pf1N1B4_2050 0.60 -1.0 2,337,212 + Pf1N1B4_2050 0.70 -0.9 2,337,212 + Pf1N1B4_2050 0.70 +1.8 2,337,257 + Pf1N1B4_2050 0.74 +0.3 2,337,257 + Pf1N1B4_2050 0.74 -1.5 2,337,257 + Pf1N1B4_2050 0.74 +0.5 2,337,257 + Pf1N1B4_2050 0.74 -0.5 2,337,257 + Pf1N1B4_2050 0.74 -0.5 2,337,257 + Pf1N1B4_2050 0.74 +0.5 2,337,257 + Pf1N1B4_2050 0.74 -0.7 2,337,258 - Pf1N1B4_2050 0.74 -0.2 2,337,258 - Pf1N1B4_2050 0.74 -0.9 2,337,258 - Pf1N1B4_2050 0.74 +0.9 2,337,258 - Pf1N1B4_2050 0.74 +0.1 2,337,317 - Pf1N1B4_2050 0.80 -0.2 2,337,317 - Pf1N1B4_2050 0.80 -1.5 2,337,329 + Pf1N1B4_2050 0.81 -1.5 2,337,472 - -0.0 2,337,479 + -0.9 2,337,548 + -1.2 2,337,549 - -0.5 2,337,552 + +0.2 2,337,622 - +1.1 2,337,659 + +0.3 2,337,710 + -0.1 2,337,710 + -0.0 2,337,711 - +0.5 2,337,711 - -0.2 2,337,711 - -0.1 2,337,711 - -1.1 2,337,711 - -0.5 2,337,741 + +0.1 2,337,741 + -0.1 2,337,741 + +0.9 2,337,748 + -0.2 2,338,016 + +1.0 2,338,139 + Pf1N1B4_2052 0.10 +0.2 2,338,179 + Pf1N1B4_2052 0.29 +2.1 2,338,179 + Pf1N1B4_2052 0.29 +1.0 2,338,180 - Pf1N1B4_2052 0.30 +0.8 2,338,180 - Pf1N1B4_2052 0.30 -0.5 2,338,257 + Pf1N1B4_2052 0.66 +0.5 2,338,257 + Pf1N1B4_2052 0.66 +1.1 2,338,258 - Pf1N1B4_2052 0.67 +0.5 2,338,258 - Pf1N1B4_2052 0.67 -0.9 2,338,258 - Pf1N1B4_2052 0.67 -0.0 2,338,266 + Pf1N1B4_2052 0.70 -0.6 2,338,281 - Pf1N1B4_2052 0.78 +1.3 2,338,281 - Pf1N1B4_2052 0.78 +0.5 2,338,281 - Pf1N1B4_2052 0.78 +1.3 2,338,281 - Pf1N1B4_2052 0.78 +0.5 2,338,281 - Pf1N1B4_2052 0.78 +0.5 2,338,361 + +0.1 2,338,362 - +1.9 2,338,415 + -0.0 2,338,416 - +0.5 2,338,416 - +0.1 2,338,416 - -0.5 2,338,416 - +0.5 2,338,416 - -1.9 2,338,416 - +2.5 2,338,461 + +0.3 2,338,461 + +0.5 2,338,461 + -1.3 2,338,462 - +0.4 2,338,462 - +1.1 2,338,462 - -0.1 2,338,462 - +1.7 2,338,462 - +0.2 2,338,462 - +0.5 2,338,462 - +2.1 2,338,495 + +1.8
Or see this region's nucleotide sequence