Experiment: Thallium(I) acetate 0.008 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1473 and Pf1N1B4_1474 are separated by 316 nucleotides Pf1N1B4_1474 and Pf1N1B4_1475 are separated by 216 nucleotides Pf1N1B4_1475 and Pf1N1B4_1476 overlap by 6 nucleotides
Pf1N1B4_1473 - ADP-heptose--lipooligosaccharide heptosyltransferase II (EC 2.4.1.-), at 1,687,770 to 1,688,804
_1473
Pf1N1B4_1474 - Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42), at 1,689,121 to 1,692,060
_1474
Pf1N1B4_1475 - FIG00962376: hypothetical protein, at 1,692,277 to 1,692,450
_1475
Pf1N1B4_1476 - Pyruvate dehydrogenase E1 component (EC 1.2.4.1), at 1,692,445 to 1,695,090
_1476
Position (kb)
1689
1690
1691
1692
1693 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1688.835 kb on - strand at 1688.922 kb on - strand at 1688.922 kb on - strand at 1688.922 kb on - strand at 1688.922 kb on - strand at 1688.925 kb on - strand at 1689.194 kb on - strand at 1689.248 kb on + strand at 1689.249 kb on - strand at 1689.249 kb on - strand at 1689.310 kb on + strand at 1689.310 kb on + strand at 1689.311 kb on - strand at 1689.311 kb on - strand at 1689.378 kb on - strand at 1689.387 kb on + strand at 1689.405 kb on + strand at 1689.749 kb on + strand, within Pf1N1B4_1474 at 1689.901 kb on - strand, within Pf1N1B4_1474 at 1689.901 kb on - strand, within Pf1N1B4_1474 at 1689.993 kb on - strand, within Pf1N1B4_1474 at 1690.377 kb on - strand, within Pf1N1B4_1474 at 1690.739 kb on + strand, within Pf1N1B4_1474 at 1690.785 kb on + strand, within Pf1N1B4_1474 at 1690.786 kb on - strand, within Pf1N1B4_1474 at 1690.957 kb on + strand, within Pf1N1B4_1474 at 1690.958 kb on - strand, within Pf1N1B4_1474 at 1690.960 kb on + strand, within Pf1N1B4_1474 at 1690.961 kb on - strand, within Pf1N1B4_1474 at 1691.268 kb on - strand, within Pf1N1B4_1474 at 1691.268 kb on - strand, within Pf1N1B4_1474 at 1691.271 kb on - strand, within Pf1N1B4_1474 at 1691.271 kb on - strand, within Pf1N1B4_1474 at 1691.271 kb on - strand, within Pf1N1B4_1474 at 1691.603 kb on + strand, within Pf1N1B4_1474 at 1691.603 kb on + strand, within Pf1N1B4_1474 at 1691.604 kb on - strand, within Pf1N1B4_1474 at 1691.604 kb on - strand, within Pf1N1B4_1474 at 1691.604 kb on - strand, within Pf1N1B4_1474 at 1691.604 kb on - strand, within Pf1N1B4_1474 at 1692.026 kb on - strand at 1692.026 kb on - strand at 1692.066 kb on - strand at 1692.096 kb on + strand at 1692.096 kb on + strand at 1692.097 kb on - strand at 1692.097 kb on - strand at 1692.097 kb on - strand at 1692.228 kb on + strand at 1692.252 kb on + strand at 1692.275 kb on + strand at 1692.288 kb on + strand at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.347 kb on + strand, within Pf1N1B4_1475 at 1692.348 kb on - strand, within Pf1N1B4_1475 at 1692.370 kb on + strand, within Pf1N1B4_1475 at 1692.370 kb on + strand, within Pf1N1B4_1475 at 1692.370 kb on + strand, within Pf1N1B4_1475 at 1692.370 kb on + strand, within Pf1N1B4_1475 at 1692.370 kb on + strand, within Pf1N1B4_1475 at 1692.796 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Thallium(I) acetate 0.008 mg/ml remove 1,688,835 - +0.1 1,688,922 - -1.6 1,688,922 - -1.0 1,688,922 - -1.2 1,688,922 - +0.6 1,688,925 - +0.1 1,689,194 - -0.2 1,689,248 + +0.7 1,689,249 - -0.2 1,689,249 - -2.2 1,689,310 + -1.6 1,689,310 + -1.5 1,689,311 - +0.4 1,689,311 - +1.4 1,689,378 - +2.2 1,689,387 + +0.4 1,689,405 + -0.1 1,689,749 + Pf1N1B4_1474 0.21 -0.1 1,689,901 - Pf1N1B4_1474 0.27 +0.4 1,689,901 - Pf1N1B4_1474 0.27 -0.6 1,689,993 - Pf1N1B4_1474 0.30 +1.4 1,690,377 - Pf1N1B4_1474 0.43 -0.6 1,690,739 + Pf1N1B4_1474 0.55 +0.9 1,690,785 + Pf1N1B4_1474 0.57 -2.0 1,690,786 - Pf1N1B4_1474 0.57 -1.6 1,690,957 + Pf1N1B4_1474 0.62 +0.7 1,690,958 - Pf1N1B4_1474 0.62 +0.4 1,690,960 + Pf1N1B4_1474 0.63 -2.0 1,690,961 - Pf1N1B4_1474 0.63 +0.4 1,691,268 - Pf1N1B4_1474 0.73 +1.7 1,691,268 - Pf1N1B4_1474 0.73 -0.6 1,691,271 - Pf1N1B4_1474 0.73 -1.4 1,691,271 - Pf1N1B4_1474 0.73 -0.6 1,691,271 - Pf1N1B4_1474 0.73 -1.2 1,691,603 + Pf1N1B4_1474 0.84 +0.4 1,691,603 + Pf1N1B4_1474 0.84 -0.5 1,691,604 - Pf1N1B4_1474 0.84 -0.2 1,691,604 - Pf1N1B4_1474 0.84 -2.0 1,691,604 - Pf1N1B4_1474 0.84 +0.4 1,691,604 - Pf1N1B4_1474 0.84 -2.0 1,692,026 - -0.4 1,692,026 - -0.8 1,692,066 - -1.6 1,692,096 + -1.1 1,692,096 + -1.5 1,692,097 - -2.1 1,692,097 - -1.9 1,692,097 - -1.0 1,692,228 + +0.9 1,692,252 + +0.4 1,692,275 + -0.2 1,692,288 + -0.0 1,692,347 + Pf1N1B4_1475 0.40 +0.4 1,692,347 + Pf1N1B4_1475 0.40 -1.2 1,692,347 + Pf1N1B4_1475 0.40 -1.6 1,692,347 + Pf1N1B4_1475 0.40 -0.1 1,692,347 + Pf1N1B4_1475 0.40 -1.6 1,692,347 + Pf1N1B4_1475 0.40 -1.0 1,692,347 + Pf1N1B4_1475 0.40 +0.8 1,692,347 + Pf1N1B4_1475 0.40 -2.1 1,692,347 + Pf1N1B4_1475 0.40 -2.6 1,692,347 + Pf1N1B4_1475 0.40 +0.1 1,692,347 + Pf1N1B4_1475 0.40 -1.2 1,692,347 + Pf1N1B4_1475 0.40 -0.8 1,692,347 + Pf1N1B4_1475 0.40 -2.0 1,692,347 + Pf1N1B4_1475 0.40 -3.0 1,692,348 - Pf1N1B4_1475 0.41 +1.1 1,692,370 + Pf1N1B4_1475 0.53 -2.0 1,692,370 + Pf1N1B4_1475 0.53 -0.5 1,692,370 + Pf1N1B4_1475 0.53 -1.2 1,692,370 + Pf1N1B4_1475 0.53 +1.7 1,692,370 + Pf1N1B4_1475 0.53 -0.6 1,692,796 + -1.2
Or see this region's nucleotide sequence