Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03445 and MMP_RS03450 are separated by 74 nucleotides MMP_RS03450 and MMP_RS03455 are separated by 97 nucleotides MMP_RS03455 and MMP_RS03460 are separated by 91 nucleotides
MMP_RS03445: MMP_RS03445 - methanogenesis marker 17 protein, at 647,049 to 647,600
_RS03445
MMP_RS03450: MMP_RS03450 - glycine synthesis protein GlyXS <small>(from data)</small>, at 647,675 to 647,947
_RS03450
MMP_RS03455: MMP_RS03455 - radical SAM protein, at 648,045 to 648,938
_RS03455
MMP_RS03460: MMP_RS03460 - U32 family peptidase, at 649,030 to 650,241
_RS03460
Position (kb) 648
649 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1 at 647.055 kb on - strand at 647.407 kb on - strand, within MMP_RS03445 at 647.695 kb on - strand at 647.797 kb on - strand, within MMP_RS03450 at 647.797 kb on - strand, within MMP_RS03450 at 647.852 kb on - strand, within MMP_RS03450 at 647.951 kb on - strand at 647.951 kb on - strand at 648.008 kb on - strand at 648.008 kb on - strand at 648.009 kb on + strand at 648.015 kb on - strand at 648.086 kb on - strand at 648.087 kb on + strand at 648.117 kb on - strand at 648.186 kb on + strand at 648.261 kb on - strand at 648.262 kb on + strand at 648.262 kb on + strand at 648.508 kb on + strand at 648.718 kb on - strand at 648.902 kb on - strand at 648.903 kb on + strand at 648.905 kb on - strand at 648.927 kb on + strand at 649.014 kb on - strand at 649.019 kb on - strand at 649.032 kb on + strand at 649.042 kb on - strand at 649.043 kb on + strand at 649.043 kb on + strand at 649.050 kb on + strand at 649.116 kb on - strand at 649.173 kb on - strand, within MMP_RS03460 at 649.173 kb on - strand, within MMP_RS03460 at 649.222 kb on + strand, within MMP_RS03460 at 649.252 kb on + strand, within MMP_RS03460 at 649.339 kb on + strand, within MMP_RS03460 at 649.367 kb on - strand, within MMP_RS03460 at 649.405 kb on - strand, within MMP_RS03460 at 649.424 kb on - strand, within MMP_RS03460 at 649.425 kb on + strand, within MMP_RS03460 at 649.485 kb on + strand, within MMP_RS03460 at 649.535 kb on + strand, within MMP_RS03460 at 649.693 kb on + strand, within MMP_RS03460 at 649.696 kb on - strand, within MMP_RS03460 at 649.768 kb on - strand, within MMP_RS03460 at 649.769 kb on + strand, within MMP_RS03460
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 & Casamino Acid FREE remove 647,055 - -2.6 647,407 - MMP_RS03445 0.65 -3.5 647,695 - -4.1 647,797 - MMP_RS03450 0.45 -4.5 647,797 - MMP_RS03450 0.45 -5.9 647,852 - MMP_RS03450 0.65 -5.7 647,951 - -5.3 647,951 - -4.3 648,008 - -5.7 648,008 - -6.5 648,009 + -6.1 648,015 - -4.9 648,086 - -0.3 648,087 + -0.2 648,117 - -0.1 648,186 + -4.2 648,261 - -2.2 648,262 + -2.8 648,262 + -4.3 648,508 + -4.3 648,718 - -3.8 648,902 - -4.4 648,903 + -2.8 648,905 - -2.1 648,927 + -2.5 649,014 - -0.8 649,019 - +0.0 649,032 + -0.0 649,042 - +0.3 649,043 + +0.3 649,043 + +0.1 649,050 + +0.2 649,116 - -0.4 649,173 - MMP_RS03460 0.12 +0.4 649,173 - MMP_RS03460 0.12 +0.0 649,222 + MMP_RS03460 0.16 +0.3 649,252 + MMP_RS03460 0.18 +0.0 649,339 + MMP_RS03460 0.25 -0.4 649,367 - MMP_RS03460 0.28 +0.1 649,405 - MMP_RS03460 0.31 -0.3 649,424 - MMP_RS03460 0.33 -0.3 649,425 + MMP_RS03460 0.33 -0.3 649,485 + MMP_RS03460 0.38 -0.6 649,535 + MMP_RS03460 0.42 -0.1 649,693 + MMP_RS03460 0.55 -0.7 649,696 - MMP_RS03460 0.55 +0.2 649,768 - MMP_RS03460 0.61 +0.1 649,769 + MMP_RS03460 0.61 +0.8
Or see this region's nucleotide sequence