Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS04130 and MMJJ_RS04135 are separated by 131 nucleotides MMJJ_RS04135 and MMJJ_RS04140 are separated by 15 nucleotides
MMJJ_RS04130: MMJJ_RS04130 - anaerobic ribonucleoside-triphosphate reductase, at 766,183 to 768,495
_RS04130
MMJJ_RS04135: MMJJ_RS04135 - 7-cyano-7-deazaguanine synthase QueC, at 768,627 to 769,328
_RS04135
MMJJ_RS04140: MMJJ_RS04140 - iron-containing alcohol dehydrogenase, at 769,344 to 770,348
_RS04140
Position (kb) 768
769
770 Strain fitness (log2 ratio) -1
0
1 at 768.471 kb on - strand at 768.472 kb on + strand at 768.504 kb on + strand at 768.519 kb on - strand at 768.558 kb on + strand at 768.558 kb on + strand at 768.558 kb on + strand at 768.563 kb on + strand at 768.589 kb on + strand at 768.590 kb on - strand at 768.590 kb on - strand at 768.591 kb on + strand at 768.602 kb on + strand at 768.606 kb on - strand at 768.607 kb on + strand at 768.607 kb on + strand at 768.611 kb on - strand at 768.636 kb on + strand at 768.636 kb on + strand at 768.643 kb on - strand at 768.644 kb on + strand at 768.644 kb on + strand at 768.671 kb on + strand at 768.717 kb on + strand, within MMJJ_RS04135 at 768.717 kb on + strand, within MMJJ_RS04135 at 768.743 kb on + strand, within MMJJ_RS04135 at 768.743 kb on + strand, within MMJJ_RS04135 at 768.755 kb on + strand, within MMJJ_RS04135 at 768.755 kb on + strand, within MMJJ_RS04135 at 768.772 kb on - strand, within MMJJ_RS04135 at 768.784 kb on - strand, within MMJJ_RS04135 at 768.785 kb on + strand, within MMJJ_RS04135 at 768.803 kb on + strand, within MMJJ_RS04135 at 769.083 kb on - strand, within MMJJ_RS04135 at 769.088 kb on - strand, within MMJJ_RS04135 at 769.094 kb on + strand, within MMJJ_RS04135 at 769.121 kb on + strand, within MMJJ_RS04135 at 769.122 kb on - strand, within MMJJ_RS04135 at 769.123 kb on + strand, within MMJJ_RS04135 at 769.198 kb on + strand, within MMJJ_RS04135 at 769.203 kb on - strand, within MMJJ_RS04135 at 769.204 kb on + strand, within MMJJ_RS04135 at 769.237 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 768,471 - -0.1 768,472 + +0.6 768,504 + +0.2 768,519 - +0.1 768,558 + +0.4 768,558 + +0.3 768,558 + +0.0 768,563 + +1.2 768,589 + -0.5 768,590 - +0.2 768,590 - +0.6 768,591 + +0.1 768,602 + -0.0 768,606 - +0.5 768,607 + +0.1 768,607 + +0.3 768,611 - +0.1 768,636 + -0.4 768,636 + +0.1 768,643 - -1.4 768,644 + -0.4 768,644 + +0.5 768,671 + +0.9 768,717 + MMJJ_RS04135 0.13 +0.4 768,717 + MMJJ_RS04135 0.13 -0.7 768,743 + MMJJ_RS04135 0.17 +0.3 768,743 + MMJJ_RS04135 0.17 +0.1 768,755 + MMJJ_RS04135 0.18 -1.3 768,755 + MMJJ_RS04135 0.18 +0.0 768,772 - MMJJ_RS04135 0.21 -0.2 768,784 - MMJJ_RS04135 0.22 -0.1 768,785 + MMJJ_RS04135 0.23 +0.2 768,803 + MMJJ_RS04135 0.25 +0.4 769,083 - MMJJ_RS04135 0.65 +0.9 769,088 - MMJJ_RS04135 0.66 +0.5 769,094 + MMJJ_RS04135 0.67 +0.8 769,121 + MMJJ_RS04135 0.70 -0.0 769,122 - MMJJ_RS04135 0.71 -0.5 769,123 + MMJJ_RS04135 0.71 +0.2 769,198 + MMJJ_RS04135 0.81 +0.4 769,203 - MMJJ_RS04135 0.82 +0.6 769,204 + MMJJ_RS04135 0.82 +0.4 769,237 - +0.9
Or see this region's nucleotide sequence