Experiment: H2 McCas, 5g/L bicarb, 40 psi H2CO2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS02310 and MMJJ_RS02315 are separated by 88 nucleotides MMJJ_RS02315 and MMJJ_RS02320 are separated by 119 nucleotides MMJJ_RS02320 and MMJJ_RS02325 are separated by 97 nucleotides
MMJJ_RS02310: MMJJ_RS02310 - ABC transporter ATP-binding protein, at 436,273 to 436,977
_RS02310
MMJJ_RS02315: MMJJ_RS02315 - magnesium chelatase, at 437,066 to 437,710
_RS02315
MMJJ_RS02320: MMJJ_RS02320 - VWA domain-containing protein, at 437,830 to 438,564
_RS02320
MMJJ_RS02325: MMJJ_RS02325 - hypothetical protein, at 438,662 to 439,618
_RS02325
Position (kb) 437
438
439 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 436.863 kb on - strand, within MMJJ_RS02310 at 436.863 kb on - strand, within MMJJ_RS02310 at 436.866 kb on - strand, within MMJJ_RS02310 at 436.872 kb on - strand, within MMJJ_RS02310 at 436.873 kb on + strand, within MMJJ_RS02310 at 436.873 kb on + strand, within MMJJ_RS02310 at 436.873 kb on + strand, within MMJJ_RS02310 at 436.898 kb on + strand, within MMJJ_RS02310 at 436.919 kb on - strand at 436.942 kb on + strand at 436.942 kb on + strand at 436.942 kb on + strand at 436.944 kb on + strand at 436.944 kb on + strand at 436.944 kb on + strand at 436.979 kb on + strand at 436.988 kb on + strand at 437.028 kb on - strand at 437.054 kb on - strand at 437.055 kb on + strand at 437.082 kb on + strand at 437.087 kb on + strand at 437.153 kb on + strand, within MMJJ_RS02315 at 437.175 kb on - strand, within MMJJ_RS02315 at 437.176 kb on + strand, within MMJJ_RS02315 at 437.185 kb on - strand, within MMJJ_RS02315 at 437.185 kb on - strand, within MMJJ_RS02315 at 437.186 kb on + strand, within MMJJ_RS02315 at 437.221 kb on + strand, within MMJJ_RS02315 at 437.221 kb on + strand, within MMJJ_RS02315 at 437.227 kb on - strand, within MMJJ_RS02315 at 437.240 kb on - strand, within MMJJ_RS02315 at 437.240 kb on - strand, within MMJJ_RS02315 at 437.290 kb on + strand, within MMJJ_RS02315 at 437.330 kb on - strand, within MMJJ_RS02315 at 437.339 kb on - strand, within MMJJ_RS02315 at 437.339 kb on - strand, within MMJJ_RS02315 at 437.340 kb on + strand, within MMJJ_RS02315 at 437.422 kb on + strand, within MMJJ_RS02315 at 437.446 kb on + strand, within MMJJ_RS02315 at 437.446 kb on + strand, within MMJJ_RS02315 at 437.481 kb on - strand, within MMJJ_RS02315 at 437.490 kb on - strand, within MMJJ_RS02315 at 437.527 kb on - strand, within MMJJ_RS02315 at 437.527 kb on - strand, within MMJJ_RS02315 at 437.536 kb on - strand, within MMJJ_RS02315 at 437.537 kb on + strand, within MMJJ_RS02315 at 437.550 kb on - strand, within MMJJ_RS02315 at 437.586 kb on - strand, within MMJJ_RS02315 at 437.587 kb on + strand, within MMJJ_RS02315 at 437.587 kb on + strand, within MMJJ_RS02315 at 437.591 kb on + strand, within MMJJ_RS02315 at 437.635 kb on - strand, within MMJJ_RS02315 at 437.688 kb on - strand at 437.708 kb on - strand at 437.770 kb on + strand at 437.879 kb on - strand at 437.918 kb on + strand, within MMJJ_RS02320 at 437.919 kb on - strand, within MMJJ_RS02320 at 437.919 kb on - strand, within MMJJ_RS02320 at 437.920 kb on + strand, within MMJJ_RS02320 at 437.920 kb on + strand, within MMJJ_RS02320 at 437.920 kb on + strand, within MMJJ_RS02320 at 437.954 kb on - strand, within MMJJ_RS02320 at 437.955 kb on + strand, within MMJJ_RS02320 at 438.158 kb on - strand, within MMJJ_RS02320 at 438.169 kb on - strand, within MMJJ_RS02320 at 438.170 kb on + strand, within MMJJ_RS02320 at 438.172 kb on + strand, within MMJJ_RS02320 at 438.210 kb on + strand, within MMJJ_RS02320 at 438.222 kb on - strand, within MMJJ_RS02320 at 438.223 kb on + strand, within MMJJ_RS02320 at 438.239 kb on - strand, within MMJJ_RS02320 at 438.240 kb on + strand, within MMJJ_RS02320 at 438.301 kb on + strand, within MMJJ_RS02320 at 438.312 kb on + strand, within MMJJ_RS02320 at 438.371 kb on - strand, within MMJJ_RS02320 at 438.371 kb on - strand, within MMJJ_RS02320 at 438.410 kb on - strand, within MMJJ_RS02320 at 438.410 kb on - strand, within MMJJ_RS02320 at 438.465 kb on - strand, within MMJJ_RS02320 at 438.471 kb on + strand, within MMJJ_RS02320 at 438.471 kb on + strand, within MMJJ_RS02320 at 438.473 kb on + strand, within MMJJ_RS02320 at 438.473 kb on + strand, within MMJJ_RS02320 at 438.486 kb on - strand, within MMJJ_RS02320 at 438.507 kb on - strand at 438.507 kb on - strand at 438.508 kb on + strand at 438.508 kb on + strand at 438.508 kb on + strand at 438.508 kb on + strand at 438.558 kb on - strand at 438.563 kb on + strand at 438.563 kb on + strand at 438.566 kb on - strand at 438.566 kb on - strand at 438.567 kb on + strand at 438.614 kb on - strand at 438.627 kb on + strand at 438.631 kb on - strand at 438.645 kb on - strand at 438.690 kb on + strand at 438.692 kb on - strand at 438.692 kb on - strand at 438.693 kb on + strand at 438.695 kb on - strand at 438.695 kb on - strand at 438.696 kb on + strand at 438.696 kb on + strand at 438.696 kb on + strand at 438.697 kb on - strand at 438.712 kb on - strand at 438.712 kb on - strand at 438.713 kb on + strand at 438.715 kb on - strand at 438.715 kb on - strand at 438.715 kb on - strand at 438.716 kb on + strand at 438.716 kb on + strand at 438.716 kb on + strand at 438.796 kb on + strand, within MMJJ_RS02325 at 438.812 kb on - strand, within MMJJ_RS02325 at 438.814 kb on - strand, within MMJJ_RS02325 at 438.815 kb on + strand, within MMJJ_RS02325 at 438.830 kb on - strand, within MMJJ_RS02325 at 438.914 kb on - strand, within MMJJ_RS02325 at 438.960 kb on - strand, within MMJJ_RS02325 at 439.143 kb on - strand, within MMJJ_RS02325 at 439.149 kb on + strand, within MMJJ_RS02325 at 439.154 kb on + strand, within MMJJ_RS02325 at 439.176 kb on - strand, within MMJJ_RS02325 at 439.177 kb on + strand, within MMJJ_RS02325 at 439.181 kb on - strand, within MMJJ_RS02325 at 439.182 kb on + strand, within MMJJ_RS02325 at 439.189 kb on - strand, within MMJJ_RS02325 at 439.189 kb on - strand, within MMJJ_RS02325 at 439.228 kb on - strand, within MMJJ_RS02325 at 439.229 kb on + strand, within MMJJ_RS02325 at 439.229 kb on + strand, within MMJJ_RS02325 at 439.229 kb on + strand, within MMJJ_RS02325 at 439.230 kb on - strand, within MMJJ_RS02325 at 439.230 kb on - strand, within MMJJ_RS02325 at 439.230 kb on - strand, within MMJJ_RS02325 at 439.230 kb on - strand, within MMJJ_RS02325 at 439.230 kb on - strand, within MMJJ_RS02325 at 439.231 kb on + strand, within MMJJ_RS02325 at 439.231 kb on + strand, within MMJJ_RS02325 at 439.231 kb on + strand, within MMJJ_RS02325 at 439.232 kb on - strand, within MMJJ_RS02325 at 439.232 kb on - strand, within MMJJ_RS02325 at 439.233 kb on + strand, within MMJJ_RS02325 at 439.233 kb on + strand, within MMJJ_RS02325 at 439.233 kb on + strand, within MMJJ_RS02325 at 439.233 kb on + strand, within MMJJ_RS02325 at 439.233 kb on + strand, within MMJJ_RS02325 at 439.234 kb on - strand, within MMJJ_RS02325 at 439.234 kb on - strand, within MMJJ_RS02325 at 439.358 kb on + strand, within MMJJ_RS02325 at 439.363 kb on - strand, within MMJJ_RS02325 at 439.363 kb on - strand, within MMJJ_RS02325 at 439.363 kb on - strand, within MMJJ_RS02325 at 439.363 kb on - strand, within MMJJ_RS02325 at 439.363 kb on - strand, within MMJJ_RS02325 at 439.364 kb on + strand, within MMJJ_RS02325 at 439.364 kb on + strand, within MMJJ_RS02325 at 439.365 kb on - strand, within MMJJ_RS02325 at 439.365 kb on - strand, within MMJJ_RS02325 at 439.380 kb on + strand at 439.380 kb on + strand, within MMJJ_RS02325 at 439.386 kb on - strand, within MMJJ_RS02325 at 439.425 kb on - strand, within MMJJ_RS02325 at 439.426 kb on + strand, within MMJJ_RS02325 at 439.428 kb on + strand, within MMJJ_RS02325 at 439.448 kb on - strand, within MMJJ_RS02325 at 439.449 kb on + strand, within MMJJ_RS02325 at 439.467 kb on - strand, within MMJJ_RS02325 at 439.481 kb on - strand, within MMJJ_RS02325 at 439.482 kb on + strand, within MMJJ_RS02325 at 439.482 kb on + strand, within MMJJ_RS02325 at 439.494 kb on + strand, within MMJJ_RS02325 at 439.502 kb on + strand, within MMJJ_RS02325 at 439.506 kb on + strand, within MMJJ_RS02325 at 439.531 kb on - strand at 439.532 kb on + strand at 439.551 kb on + strand at 439.556 kb on - strand at 439.557 kb on + strand at 439.557 kb on + strand at 439.557 kb on + strand at 439.561 kb on - strand at 439.561 kb on - strand at 439.562 kb on + strand at 439.562 kb on + strand at 439.563 kb on - strand at 439.563 kb on - strand at 439.564 kb on + strand at 439.564 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 5g/L bicarb, 40 psi H2CO2 remove 436,863 - MMJJ_RS02310 0.84 -2.8 436,863 - MMJJ_RS02310 0.84 -0.3 436,866 - MMJJ_RS02310 0.84 -0.5 436,872 - MMJJ_RS02310 0.85 -0.3 436,873 + MMJJ_RS02310 0.85 +0.2 436,873 + MMJJ_RS02310 0.85 -0.1 436,873 + MMJJ_RS02310 0.85 -0.3 436,898 + MMJJ_RS02310 0.89 +0.2 436,919 - +0.2 436,942 + -0.5 436,942 + +0.3 436,942 + -0.8 436,944 + -0.3 436,944 + +0.4 436,944 + -0.1 436,979 + -0.1 436,988 + +0.3 437,028 - -0.4 437,054 - -0.6 437,055 + -0.3 437,082 + -0.7 437,087 + +0.3 437,153 + MMJJ_RS02315 0.13 +1.2 437,175 - MMJJ_RS02315 0.17 -0.5 437,176 + MMJJ_RS02315 0.17 +0.0 437,185 - MMJJ_RS02315 0.18 +0.3 437,185 - MMJJ_RS02315 0.18 +0.0 437,186 + MMJJ_RS02315 0.19 -0.0 437,221 + MMJJ_RS02315 0.24 -0.0 437,221 + MMJJ_RS02315 0.24 -0.5 437,227 - MMJJ_RS02315 0.25 +1.1 437,240 - MMJJ_RS02315 0.27 +0.8 437,240 - MMJJ_RS02315 0.27 -0.3 437,290 + MMJJ_RS02315 0.35 +0.3 437,330 - MMJJ_RS02315 0.41 -0.4 437,339 - MMJJ_RS02315 0.42 -0.2 437,339 - MMJJ_RS02315 0.42 -0.2 437,340 + MMJJ_RS02315 0.42 -0.2 437,422 + MMJJ_RS02315 0.55 +0.1 437,446 + MMJJ_RS02315 0.59 -0.0 437,446 + MMJJ_RS02315 0.59 +0.3 437,481 - MMJJ_RS02315 0.64 +0.1 437,490 - MMJJ_RS02315 0.66 +0.2 437,527 - MMJJ_RS02315 0.71 -0.1 437,527 - MMJJ_RS02315 0.71 +0.2 437,536 - MMJJ_RS02315 0.73 +0.3 437,537 + MMJJ_RS02315 0.73 +0.2 437,550 - MMJJ_RS02315 0.75 +0.2 437,586 - MMJJ_RS02315 0.81 -1.0 437,587 + MMJJ_RS02315 0.81 +0.4 437,587 + MMJJ_RS02315 0.81 -0.7 437,591 + MMJJ_RS02315 0.81 -0.2 437,635 - MMJJ_RS02315 0.88 +0.1 437,688 - -0.1 437,708 - +0.2 437,770 + -0.2 437,879 - +0.6 437,918 + MMJJ_RS02320 0.12 +0.7 437,919 - MMJJ_RS02320 0.12 +1.0 437,919 - MMJJ_RS02320 0.12 -1.0 437,920 + MMJJ_RS02320 0.12 +0.9 437,920 + MMJJ_RS02320 0.12 -0.3 437,920 + MMJJ_RS02320 0.12 -0.1 437,954 - MMJJ_RS02320 0.17 +0.0 437,955 + MMJJ_RS02320 0.17 +0.8 438,158 - MMJJ_RS02320 0.45 +0.1 438,169 - MMJJ_RS02320 0.46 +0.4 438,170 + MMJJ_RS02320 0.46 +0.5 438,172 + MMJJ_RS02320 0.47 -0.1 438,210 + MMJJ_RS02320 0.52 +0.5 438,222 - MMJJ_RS02320 0.53 -0.4 438,223 + MMJJ_RS02320 0.53 +0.8 438,239 - MMJJ_RS02320 0.56 -0.1 438,240 + MMJJ_RS02320 0.56 -0.4 438,301 + MMJJ_RS02320 0.64 -1.1 438,312 + MMJJ_RS02320 0.66 +0.1 438,371 - MMJJ_RS02320 0.74 +0.6 438,371 - MMJJ_RS02320 0.74 +0.0 438,410 - MMJJ_RS02320 0.79 -0.1 438,410 - MMJJ_RS02320 0.79 -0.7 438,465 - MMJJ_RS02320 0.86 -0.3 438,471 + MMJJ_RS02320 0.87 +0.3 438,471 + MMJJ_RS02320 0.87 +0.1 438,473 + MMJJ_RS02320 0.87 +0.4 438,473 + MMJJ_RS02320 0.87 +1.2 438,486 - MMJJ_RS02320 0.89 -3.2 438,507 - +0.1 438,507 - -0.6 438,508 + +0.3 438,508 + -0.0 438,508 + +0.3 438,508 + +0.0 438,558 - +0.1 438,563 + -0.5 438,563 + +0.4 438,566 - -0.2 438,566 - -0.1 438,567 + +0.5 438,614 - +0.2 438,627 + -0.2 438,631 - -0.9 438,645 - +0.1 438,690 + +0.3 438,692 - +0.3 438,692 - -0.1 438,693 + -0.2 438,695 - -0.1 438,695 - -0.2 438,696 + +0.8 438,696 + -0.8 438,696 + -0.3 438,697 - -0.8 438,712 - +1.9 438,712 - -0.7 438,713 + +0.1 438,715 - -0.7 438,715 - -0.3 438,715 - -1.0 438,716 + +0.1 438,716 + -0.1 438,716 + +0.2 438,796 + MMJJ_RS02325 0.14 -0.5 438,812 - MMJJ_RS02325 0.16 -0.6 438,814 - MMJJ_RS02325 0.16 -0.0 438,815 + MMJJ_RS02325 0.16 +0.3 438,830 - MMJJ_RS02325 0.18 -0.2 438,914 - MMJJ_RS02325 0.26 -0.0 438,960 - MMJJ_RS02325 0.31 +0.2 439,143 - MMJJ_RS02325 0.50 +0.1 439,149 + MMJJ_RS02325 0.51 -0.3 439,154 + MMJJ_RS02325 0.51 -1.4 439,176 - MMJJ_RS02325 0.54 +0.1 439,177 + MMJJ_RS02325 0.54 +0.0 439,181 - MMJJ_RS02325 0.54 -2.2 439,182 + MMJJ_RS02325 0.54 +0.3 439,189 - MMJJ_RS02325 0.55 -0.0 439,189 - MMJJ_RS02325 0.55 +0.2 439,228 - MMJJ_RS02325 0.59 +0.5 439,229 + MMJJ_RS02325 0.59 +0.0 439,229 + MMJJ_RS02325 0.59 -0.2 439,229 + MMJJ_RS02325 0.59 +0.2 439,230 - MMJJ_RS02325 0.59 +0.9 439,230 - MMJJ_RS02325 0.59 -0.6 439,230 - MMJJ_RS02325 0.59 +0.6 439,230 - MMJJ_RS02325 0.59 +0.9 439,230 - MMJJ_RS02325 0.59 -0.2 439,231 + MMJJ_RS02325 0.59 +0.6 439,231 + MMJJ_RS02325 0.59 -0.1 439,231 + MMJJ_RS02325 0.59 -0.4 439,232 - MMJJ_RS02325 0.60 +0.8 439,232 - MMJJ_RS02325 0.60 +0.2 439,233 + MMJJ_RS02325 0.60 -0.4 439,233 + MMJJ_RS02325 0.60 -1.5 439,233 + MMJJ_RS02325 0.60 -0.8 439,233 + MMJJ_RS02325 0.60 -0.6 439,233 + MMJJ_RS02325 0.60 -0.4 439,234 - MMJJ_RS02325 0.60 -0.6 439,234 - MMJJ_RS02325 0.60 -0.7 439,358 + MMJJ_RS02325 0.73 -0.0 439,363 - MMJJ_RS02325 0.73 +0.1 439,363 - MMJJ_RS02325 0.73 -0.2 439,363 - MMJJ_RS02325 0.73 +0.2 439,363 - MMJJ_RS02325 0.73 +0.5 439,363 - MMJJ_RS02325 0.73 -0.1 439,364 + MMJJ_RS02325 0.73 +0.1 439,364 + MMJJ_RS02325 0.73 -0.7 439,365 - MMJJ_RS02325 0.73 +0.0 439,365 - MMJJ_RS02325 0.73 +0.8 439,380 + +0.8 439,380 + MMJJ_RS02325 0.75 -1.3 439,386 - MMJJ_RS02325 0.76 +0.8 439,425 - MMJJ_RS02325 0.80 -0.1 439,426 + MMJJ_RS02325 0.80 +0.4 439,428 + MMJJ_RS02325 0.80 -0.6 439,448 - MMJJ_RS02325 0.82 -0.3 439,449 + MMJJ_RS02325 0.82 +0.3 439,467 - MMJJ_RS02325 0.84 +0.2 439,481 - MMJJ_RS02325 0.86 -0.3 439,482 + MMJJ_RS02325 0.86 -0.4 439,482 + MMJJ_RS02325 0.86 -0.5 439,494 + MMJJ_RS02325 0.87 +0.2 439,502 + MMJJ_RS02325 0.88 -0.8 439,506 + MMJJ_RS02325 0.88 -1.4 439,531 - +0.1 439,532 + -0.5 439,551 + +1.3 439,556 - -2.2 439,557 + -0.2 439,557 + +0.2 439,557 + -0.2 439,561 - -0.2 439,561 - -1.7 439,562 + -0.1 439,562 + -0.0 439,563 - +0.4 439,563 - -0.3 439,564 + -0.0 439,564 + +0.2
Or see this region's nucleotide sequence