Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25200 and CA265_RS25205 are separated by 53 nucleotides CA265_RS25205 and CA265_RS25210 are separated by 318 nucleotides CA265_RS25210 and CA265_RS25215 are separated by 217 nucleotides
CA265_RS25200: CA265_RS25200 - hypothetical protein, at 6,135,090 to 6,135,569
_RS25200
CA265_RS25205: CA265_RS25205 - hypothetical protein, at 6,135,623 to 6,136,072
_RS25205
CA265_RS25210: CA265_RS25210 - lipid-binding protein, at 6,136,391 to 6,136,978
_RS25210
CA265_RS25215: CA265_RS25215 - MBL fold hydrolase, at 6,137,196 to 6,137,966
_RS25215
Position (kb)
6136
6137 Strain fitness (log2 ratio)
-1
0
1
2
3
4 at 6135.419 kb on - strand, within CA265_RS25200 at 6135.458 kb on + strand, within CA265_RS25200 at 6135.458 kb on + strand, within CA265_RS25200 at 6135.460 kb on + strand, within CA265_RS25200 at 6135.515 kb on + strand, within CA265_RS25200 at 6135.576 kb on + strand at 6135.583 kb on - strand at 6135.594 kb on + strand at 6135.604 kb on + strand at 6135.646 kb on + strand at 6135.659 kb on + strand at 6135.668 kb on - strand, within CA265_RS25205 at 6135.694 kb on + strand, within CA265_RS25205 at 6135.736 kb on - strand, within CA265_RS25205 at 6135.740 kb on + strand, within CA265_RS25205 at 6135.740 kb on + strand, within CA265_RS25205 at 6135.748 kb on - strand, within CA265_RS25205 at 6135.846 kb on + strand, within CA265_RS25205 at 6135.993 kb on + strand, within CA265_RS25205 at 6135.994 kb on - strand, within CA265_RS25205 at 6136.029 kb on - strand at 6136.032 kb on - strand at 6136.045 kb on - strand at 6136.047 kb on - strand at 6136.125 kb on + strand at 6136.125 kb on + strand at 6136.127 kb on + strand at 6136.127 kb on + strand at 6136.128 kb on - strand at 6136.155 kb on + strand at 6136.158 kb on + strand at 6136.268 kb on - strand at 6136.284 kb on + strand at 6136.298 kb on - strand at 6136.307 kb on + strand at 6136.328 kb on + strand at 6136.329 kb on - strand at 6136.329 kb on - strand at 6136.329 kb on - strand at 6136.422 kb on + strand at 6136.435 kb on - strand at 6136.475 kb on + strand, within CA265_RS25210 at 6136.475 kb on + strand, within CA265_RS25210 at 6136.476 kb on - strand, within CA265_RS25210 at 6136.476 kb on - strand, within CA265_RS25210 at 6136.476 kb on - strand, within CA265_RS25210 at 6136.482 kb on + strand, within CA265_RS25210 at 6136.483 kb on - strand, within CA265_RS25210 at 6136.525 kb on - strand, within CA265_RS25210 at 6136.564 kb on + strand, within CA265_RS25210 at 6136.609 kb on + strand, within CA265_RS25210 at 6136.609 kb on + strand, within CA265_RS25210 at 6136.665 kb on + strand, within CA265_RS25210 at 6136.724 kb on - strand, within CA265_RS25210 at 6136.767 kb on + strand, within CA265_RS25210 at 6136.784 kb on - strand, within CA265_RS25210 at 6136.786 kb on + strand, within CA265_RS25210 at 6136.787 kb on - strand, within CA265_RS25210 at 6136.812 kb on + strand, within CA265_RS25210 at 6136.877 kb on + strand, within CA265_RS25210 at 6136.878 kb on - strand, within CA265_RS25210 at 6137.108 kb on + strand at 6137.135 kb on - strand at 6137.148 kb on + strand at 6137.148 kb on + strand at 6137.197 kb on + strand at 6137.198 kb on - strand at 6137.229 kb on + strand at 6137.230 kb on - strand at 6137.230 kb on - strand at 6137.230 kb on - strand at 6137.509 kb on - strand, within CA265_RS25215 at 6137.561 kb on - strand, within CA265_RS25215 at 6137.561 kb on - strand, within CA265_RS25215 at 6137.610 kb on + strand, within CA265_RS25215 at 6137.706 kb on + strand, within CA265_RS25215 at 6137.706 kb on + strand, within CA265_RS25215 at 6137.707 kb on - strand, within CA265_RS25215 at 6137.715 kb on + strand, within CA265_RS25215 at 6137.723 kb on + strand, within CA265_RS25215 at 6137.886 kb on + strand, within CA265_RS25215 at 6137.932 kb on + strand at 6137.933 kb on - strand at 6137.933 kb on - strand at 6137.937 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 6,135,419 - CA265_RS25200 0.69 -0.4 6,135,458 + CA265_RS25200 0.77 +0.5 6,135,458 + CA265_RS25200 0.77 +0.0 6,135,460 + CA265_RS25200 0.77 +0.2 6,135,515 + CA265_RS25200 0.89 -0.7 6,135,576 + +0.1 6,135,583 - +0.7 6,135,594 + -0.8 6,135,604 + +0.8 6,135,646 + -1.3 6,135,659 + -0.1 6,135,668 - CA265_RS25205 0.10 +0.1 6,135,694 + CA265_RS25205 0.16 -0.1 6,135,736 - CA265_RS25205 0.25 -0.1 6,135,740 + CA265_RS25205 0.26 -0.7 6,135,740 + CA265_RS25205 0.26 +0.7 6,135,748 - CA265_RS25205 0.28 +0.5 6,135,846 + CA265_RS25205 0.50 -0.8 6,135,993 + CA265_RS25205 0.82 +0.3 6,135,994 - CA265_RS25205 0.82 +0.4 6,136,029 - -0.2 6,136,032 - -0.3 6,136,045 - +2.0 6,136,047 - -1.2 6,136,125 + +0.6 6,136,125 + +0.4 6,136,127 + +0.1 6,136,127 + -0.5 6,136,128 - -0.0 6,136,155 + -0.1 6,136,158 + +1.7 6,136,268 - -0.2 6,136,284 + +0.6 6,136,298 - -1.4 6,136,307 + +0.3 6,136,328 + +0.2 6,136,329 - +0.3 6,136,329 - -0.4 6,136,329 - -0.1 6,136,422 + +0.2 6,136,435 - -0.3 6,136,475 + CA265_RS25210 0.14 -0.6 6,136,475 + CA265_RS25210 0.14 +0.7 6,136,476 - CA265_RS25210 0.14 -0.2 6,136,476 - CA265_RS25210 0.14 -0.1 6,136,476 - CA265_RS25210 0.14 -0.5 6,136,482 + CA265_RS25210 0.15 +0.7 6,136,483 - CA265_RS25210 0.16 +0.2 6,136,525 - CA265_RS25210 0.23 +1.2 6,136,564 + CA265_RS25210 0.29 +0.1 6,136,609 + CA265_RS25210 0.37 -0.7 6,136,609 + CA265_RS25210 0.37 +1.9 6,136,665 + CA265_RS25210 0.47 +0.3 6,136,724 - CA265_RS25210 0.57 -0.6 6,136,767 + CA265_RS25210 0.64 +0.2 6,136,784 - CA265_RS25210 0.67 +1.1 6,136,786 + CA265_RS25210 0.67 +0.4 6,136,787 - CA265_RS25210 0.67 -1.1 6,136,812 + CA265_RS25210 0.72 +0.2 6,136,877 + CA265_RS25210 0.83 +0.1 6,136,878 - CA265_RS25210 0.83 -0.9 6,137,108 + +0.1 6,137,135 - +0.6 6,137,148 + -0.4 6,137,148 + -0.8 6,137,197 + +0.4 6,137,198 - +0.6 6,137,229 + +2.1 6,137,230 - -1.0 6,137,230 - +0.7 6,137,230 - +1.2 6,137,509 - CA265_RS25215 0.41 +3.0 6,137,561 - CA265_RS25215 0.47 +4.1 6,137,561 - CA265_RS25215 0.47 +2.3 6,137,610 + CA265_RS25215 0.54 +4.7 6,137,706 + CA265_RS25215 0.66 +3.4 6,137,706 + CA265_RS25215 0.66 +3.6 6,137,707 - CA265_RS25215 0.66 +2.5 6,137,715 + CA265_RS25215 0.67 +2.8 6,137,723 + CA265_RS25215 0.68 +3.3 6,137,886 + CA265_RS25215 0.89 +3.1 6,137,932 + +3.2 6,137,933 - +3.2 6,137,933 - +3.5 6,137,937 - +2.6
Or see this region's nucleotide sequence