Experiment: R2A with D-Cycloserine 0.2 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS25165 and CA265_RS25170 are separated by 83 nucleotides CA265_RS25170 and CA265_RS25175 are separated by 125 nucleotides CA265_RS25175 and CA265_RS25180 are separated by 104 nucleotides
CA265_RS25165: CA265_RS25165 - glycosyl transferase, at 6,126,323 to 6,127,957
_RS25165
CA265_RS25170: CA265_RS25170 - pyridoxine 5'-phosphate synthase, at 6,128,041 to 6,128,757
_RS25170
CA265_RS25175: CA265_RS25175 - EamA family transporter, at 6,128,883 to 6,129,788
_RS25175
CA265_RS25180: CA265_RS25180 - hypothetical protein, at 6,129,893 to 6,130,456
_RS25180
Position (kb)
6128
6129
6130 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6127.934 kb on + strand at 6127.972 kb on + strand at 6127.972 kb on + strand at 6128.030 kb on - strand at 6128.755 kb on + strand at 6128.816 kb on - strand at 6128.821 kb on - strand at 6128.880 kb on + strand at 6128.881 kb on - strand at 6128.882 kb on + strand at 6128.887 kb on + strand at 6128.939 kb on + strand at 6128.949 kb on - strand at 6128.972 kb on + strand at 6128.973 kb on - strand at 6129.131 kb on - strand, within CA265_RS25175 at 6129.131 kb on - strand, within CA265_RS25175 at 6129.147 kb on + strand, within CA265_RS25175 at 6129.229 kb on - strand, within CA265_RS25175 at 6129.260 kb on - strand at 6129.364 kb on + strand, within CA265_RS25175 at 6129.423 kb on + strand, within CA265_RS25175 at 6129.423 kb on + strand, within CA265_RS25175 at 6129.429 kb on - strand, within CA265_RS25175 at 6129.517 kb on + strand, within CA265_RS25175 at 6129.599 kb on - strand, within CA265_RS25175 at 6129.631 kb on + strand, within CA265_RS25175 at 6129.632 kb on - strand, within CA265_RS25175 at 6129.677 kb on - strand, within CA265_RS25175 at 6129.792 kb on + strand at 6129.794 kb on + strand at 6129.795 kb on - strand at 6129.815 kb on + strand at 6129.815 kb on + strand at 6129.889 kb on - strand at 6129.907 kb on + strand at 6129.913 kb on + strand at 6130.025 kb on - strand, within CA265_RS25180 at 6130.025 kb on - strand, within CA265_RS25180 at 6130.054 kb on - strand, within CA265_RS25180 at 6130.067 kb on - strand, within CA265_RS25180 at 6130.076 kb on - strand, within CA265_RS25180 at 6130.182 kb on - strand, within CA265_RS25180 at 6130.195 kb on - strand, within CA265_RS25180 at 6130.237 kb on + strand, within CA265_RS25180 at 6130.238 kb on - strand, within CA265_RS25180 at 6130.262 kb on + strand, within CA265_RS25180 at 6130.304 kb on - strand, within CA265_RS25180 at 6130.426 kb on + strand at 6130.426 kb on + strand at 6130.486 kb on - strand at 6130.502 kb on + strand at 6130.513 kb on + strand at 6130.574 kb on + strand at 6130.575 kb on - strand at 6130.630 kb on - strand at 6130.652 kb on + strand at 6130.704 kb on - strand at 6130.711 kb on - strand at 6130.728 kb on + strand at 6130.739 kb on - strand at 6130.761 kb on - strand at 6130.761 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with D-Cycloserine 0.2 mg/ml remove 6,127,934 + -0.6 6,127,972 + -0.3 6,127,972 + +1.0 6,128,030 - -1.8 6,128,755 + +0.1 6,128,816 - -1.0 6,128,821 - +0.0 6,128,880 + +0.4 6,128,881 - -0.4 6,128,882 + +1.2 6,128,887 + -0.1 6,128,939 + +0.9 6,128,949 - +1.0 6,128,972 + -0.7 6,128,973 - +0.3 6,129,131 - CA265_RS25175 0.27 +0.3 6,129,131 - CA265_RS25175 0.27 -0.6 6,129,147 + CA265_RS25175 0.29 -0.2 6,129,229 - CA265_RS25175 0.38 -0.2 6,129,260 - +2.2 6,129,364 + CA265_RS25175 0.53 -0.1 6,129,423 + CA265_RS25175 0.60 -0.4 6,129,423 + CA265_RS25175 0.60 -0.1 6,129,429 - CA265_RS25175 0.60 -0.2 6,129,517 + CA265_RS25175 0.70 +0.6 6,129,599 - CA265_RS25175 0.79 +0.3 6,129,631 + CA265_RS25175 0.83 +0.2 6,129,632 - CA265_RS25175 0.83 +0.1 6,129,677 - CA265_RS25175 0.88 -0.2 6,129,792 + -0.1 6,129,794 + +0.6 6,129,795 - +0.3 6,129,815 + +0.4 6,129,815 + -0.5 6,129,889 - -0.2 6,129,907 + -0.6 6,129,913 + -0.1 6,130,025 - CA265_RS25180 0.23 +0.2 6,130,025 - CA265_RS25180 0.23 +0.3 6,130,054 - CA265_RS25180 0.29 +1.6 6,130,067 - CA265_RS25180 0.31 +0.1 6,130,076 - CA265_RS25180 0.32 -0.1 6,130,182 - CA265_RS25180 0.51 +0.2 6,130,195 - CA265_RS25180 0.54 -0.2 6,130,237 + CA265_RS25180 0.61 -0.0 6,130,238 - CA265_RS25180 0.61 +0.4 6,130,262 + CA265_RS25180 0.65 +0.2 6,130,304 - CA265_RS25180 0.73 -0.3 6,130,426 + -0.7 6,130,426 + -1.5 6,130,486 - +0.1 6,130,502 + +0.8 6,130,513 + +0.4 6,130,574 + -0.7 6,130,575 - +0.6 6,130,630 - +0.7 6,130,652 + -0.2 6,130,704 - -0.1 6,130,711 - -0.1 6,130,728 + -0.5 6,130,739 - +0.4 6,130,761 - +1.4 6,130,761 - +1.9
Or see this region's nucleotide sequence